Patents by Inventor Elaine A. Best
Elaine A. Best has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190078137Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: September 21, 2018Publication date: March 14, 2019Inventors: John C. GERDES, Elaine BEST, Jeffrey M. MARMARO
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Patent number: 10106845Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: October 14, 2016Date of Patent: October 23, 2018Assignee: Applied Biosystems, LLCInventors: John Gerdes, Elaine Best, Jeffrey M. Marmaro
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Patent number: 9648884Abstract: A sodium bicarbonate product comprises particles containing sodium bicarbonate and an organic material that is a solid at ambient temperature. The particles have a structure comprised of individual crystallites of sodium bicarbonate attached together in the particle. More than 95% by volume of the particles have a size less than 200 ?m. Particles of the product are hollow and are formed of an outer shell of the crystallites. The product may be used, for example, as a leavening agent in the production of cooked foods. The product may be produced by spray drying a solution or slurry dissolved organic material and dissolved sodium bicarbonate. The sodium bicarbonate may be present as a suspension.Type: GrantFiled: February 20, 2013Date of Patent: May 16, 2017Assignee: Eminate LimitedInventors: Sarah Gaunt, Stephen John Minter, Edna Elaine Best, Warren L. Nehmer
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Publication number: 20170130259Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: October 14, 2016Publication date: May 11, 2017Inventors: John GERDES, Elaine Best, Jeffrey M. Marmaro
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Patent number: 9481907Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: June 12, 2014Date of Patent: November 1, 2016Assignee: Applied Biosystems, LLCInventors: John C. Gerdes, Elaine Best, Jeffrey M. Marmaro
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Patent number: 9206475Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: January 5, 2015Date of Patent: December 8, 2015Assignee: APPLIED BIOSYSTEMS, LLCInventors: John C. Gerdes, Elaine Best, Jeffrey M. Marmaro
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Publication number: 20150125869Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: January 5, 2015Publication date: May 7, 2015Inventors: John C. GERDES, Elaine Best, Jeffrey M. Marmaro
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Publication number: 20150071979Abstract: A sodium bicarbonate product comprises particles containing sodium bicarbonate and an organic material that is a solid at ambient temperature. The particles have a structure comprised of individual crystallites of sodium bicarbonate attached together in the particle. More than 95% by volume of the particles have a size less than 200 ?m. Particles of the product are hollow and are formed of an outer shell of the crystallites. The product may be used, for example, as a leavening agent in the production of cooked foods. The product may be produced by spray drying a solution or slurry dissolved organic material and dissolved sodium bicarbonate. The sodium bicarbonate may be present as a suspension.Type: ApplicationFiled: February 20, 2013Publication date: March 12, 2015Inventors: Sarah Gaunt, Stephen John Minter, Edna Elaine Best, Warren L. Nehmer
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Publication number: 20140329247Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: June 12, 2014Publication date: November 6, 2014Inventors: John C. GERDES, Elaine Best, Jeffrey M. Marmaro
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Patent number: 8815546Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: September 14, 2012Date of Patent: August 26, 2014Assignee: Applied Biosystems, LLCInventors: John Gerdes, Elaine Best, Jeffrey M. Marmaro
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Patent number: 8465945Abstract: The present invention includes a method to produce a recombinant mite Group 1 protein in a methyltrophic yeast or an Escherichia coli microorganism. The present invention also relates to a recombinant mite Group 1 protein obtained by such a method, such a recombinant protein being able to selectively bind IgE or cause proliferation of a T cell that proliferate in response to a native mite Group 1 protein. Also included in the present invention is the use of such a recombinant mite Group 1 protein to detect mite allergy or to reduce an allergic response to a mite Group 1 protein. The present invention also includes novel mite Group 1 nucleic acid molecules, proteins, recombinant molecules, and recombinant cells, as well as uses thereof.Type: GrantFiled: October 2, 2006Date of Patent: June 18, 2013Assignee: Merck Patent GmbHInventors: Elaine A. Best, Martin J. McDermott
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Publication number: 20130143754Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: September 14, 2012Publication date: June 6, 2013Applicant: APPLIED BIOSYSTEMS, LLCInventors: John C. GERDES, Elaine Best, Jeffery M. Marmaro
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Patent number: 8304214Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: November 21, 2007Date of Patent: November 6, 2012Assignee: Applied Biosystems, LLCInventors: John C. Gerdes, Elaine A. Best, Jeffrey M. Marmaro
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Patent number: 7531328Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: July 7, 2005Date of Patent: May 12, 2009Assignee: Applied Biosystem, LLCInventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Publication number: 20080131897Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: November 21, 2007Publication date: June 5, 2008Applicant: APPLERA CORPORATIONInventors: John C. GERDES, Elaine Best, Jeffery M. Marmaro
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Publication number: 20070082369Abstract: The present invention includes a method to produce a recombinant mite Group 1 protein in a methyltrophic yeast or an Escherichia coli microorganism. The present invention also relates to a recombinant mite Group 1 protein obtained by such a method, such a recombinant protein being able to selectively bind IgE or cause proliferation of a T cell that proliferate in response to a native mite Group 1 protein. Also included in the present invention is the use of such a recombinant mite Group 1 protein to detect mite allergy or to reduce an allergic response to a mite Group 1 protein. The present invention also includes novel mite Group 1 nucleic acid molecules, proteins, recombinant molecules, and recombinant cells, as well as uses thereof.Type: ApplicationFiled: October 2, 2006Publication date: April 12, 2007Applicant: HESKA CORPORATIONInventors: Elaine Best, Martin McDermott
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Patent number: 7087414Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100–1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: May 19, 2003Date of Patent: August 8, 2006Assignee: Applera CorporationInventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Publication number: 20050255517Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: July 7, 2005Publication date: November 17, 2005Inventors: John Gerdes, Elaine Best, Jeffery Marmaro
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Publication number: 20050244847Abstract: Compositions and methods are provided for the in vitro amplification of nucleic acid and, in particular, for the amplification of extrachromosomal nucleic acid molecules having a molecular weight of one kilobase or greater. The design and use of primers, low ionic strength amplification buffer, low co-solvent containing buffer, and combined polymerization/ligation reaction buffers are described in this regard.Type: ApplicationFiled: November 26, 2004Publication date: November 3, 2005Inventors: Michael Domanico, Lars-Erik Peters, Preston Hradecky, Allan Roberts, David Latorra, Elaine Best
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Publication number: 20050053615Abstract: The present invention provides a method to produce variant recombinant mite Group 1 proteins with properties suitable for accelerated, specific immunotherapy. The variants of the present invention have greatly reduced IgE binding activity, but little or no loss in the ability to stimulate T cells in allergic individuals. The present invention also relates to a variant mite Group 1 protein obtained by such a method, and the use of such a variant mite Group 1 protein to reduce an allergic response to a mite Group 1 protein. The present invention also includes novel mite Group 1 nucleic acid molecules, proteins, recombinant molecules, and recombinant cells, as well as uses thereof.Type: ApplicationFiled: July 15, 2004Publication date: March 10, 2005Inventors: Elaine Best, Martin McDermott