Abstract: The present invention relates to the construction and optimization of synthetic genes from the Zymomonas mobilis L-asparaginase gene, the method for cloning same and expression thereof in Escherichia coli. The purpose of the production of said enzyme is for producing high levels of a novel L-asparaginase that can be used in L-asparaginase-based pharmaceutical compositions for treating cancer, tumours and diseases involving cell proliferation, as well as for other medical applications.

Abstract: A method for detecting aberrant phenotypes expressed by neoplastic cells includes the steps of: 1) staining one or more normal/reactive samples and one neoplastic sample with multiple combinations of monoclonal antibodies, 2) measuring fluorescence emissions associated to the stained cells, 3) storing two independent list mode data files of information on light scatter and fluorescence characteristics of each cell, 4) creating new data files by mixing list mode data from the data file containing information the neoplastic sample into the data file containing information on the normal samples, 5) defining corresponding to normal cells and areas corresponding to empty spaces in normal/reactive samples that may be occupied by tumor cells in neoplastic samples, 6) identifying events corresponding to neoplastic cells and events corresponding to normal cells coexisting in a multidimensional space, and 7) establishing the most relevant phenotypic aberrations displayed by the neoplastic cells as compared to their nor

Abstract: A method that generates new flow cytometry data files with a potentially infinite number of dimensions is described. First two or more separate flow cytometry data files containing information about events measured in different aliquots from the same sample are fused. Then, the magnitude of the values and a corresponding uncertainty measure of the differing variables between one or more of the original data files which have been fused are estimated, for each individual event contained in the fused data file, for which that variable was not directly measured in the flow cytometer. The new data file containing both data actually measured in the flow cytometer for each event of the individual original data files fused and the estimated data for those parameters not measured in a group of events from one or more of the original data files are reconstructed multidimensionally.

Abstract: A new method for detecting aberrant phenotypes expressed by neoplastic cells present in bone marrow, peripheral blood, spinal fluid and lymph nodes, comprising the steps of: 1) separately staining one or more normal/reactive samples and one neoplastic sample with identical or partially overlapping, multiple combinations of monoclonal antibodies, 2) sequentially measuring the fluorescence emissions associated to large numbers of cells stained with the monoclonal antibodies from the normal/reactive samples and the neoplastic sample, 3) storing two independent list mode data files each containing information on the specific light scatter and fluorescence characteristics of each cell analyzed, 4) creating new data files by mixing list mode data from the data file containing information about the cells present in the neoplastic sample into the data file containing information on the cells present in the normal samples, 5) defining those areas occupied by events corresponding to normal cells and those areas corresp