Abstract: A process for the stable integration of a DNA, comprising a gene that is function in a cell of a cereal plant, wherein said DNA is integrated into the nuclear genome of said cereal plant, said process comprising the steps of:(a) providing a compact embryogenic callus of said cereal plant;(b) wounding said compact embryogenic callus or treating said compact embryogenic callus with a cell wall degrading enzyme for a period of time so as not to cause a complete disruption of tissues, and transferring said DNA into the nuclear genome of a cell in said compact embryogenic callus by means of Agrobacterium-mediated transformation to generate a transformed cell; and(c) regenerating a transformed cereal plant from said transformed cell.

Abstract: The present invention provides a rapid and efficient method for transforming monocotyledonous plants. The invention particularly relates to the use of either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants. The present invention also provides novel transgenic plants obtained by the transformation method of the present invention.

Abstract: A process for the stable integration of a DNA comprising a gene that is functional in a cell of a cereal plant wherein the DNA is integrated into the nuclear genome of a cereal plant, the process comprising:(a) providing a compact embryogenic callus of the cereal plant,(b) wounding the compact embryogenic callus by cutting the callus into pieces or treating the compact embryogenic callus with a cell wall degrading enzyme for a period of time so as not to cause a complete disruption of tissues and transferring the DNA into the nuclear genome of a cell in the compact embryogenic callus by means of electroporation to generate a transformed cell, and(c) regenerating a transformed cereal plant from the transformed cell.

Abstract: A process for genetically transforming the nuclear genome of a cell of a rice plant, comprising the steps of: providing without any enzymatic pretreatment or mechanical cell wall removal a culture of aggregated suspension cells which are preplasmolyzed in a hypertonic buffer, transforming said cells by electroporating and optionally regenerating a transformed plant.

Abstract: A rapid and efficient method for transforming monocotyledonous plants, especially gramineous plants, particularly corn, using either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants, is provided. Preferably, the tissue or embryogenic callus is wounded and/or degraded prior to transformation. The resulting wounded and/or degraded, intact tissue or compact embryogenic callus is then transformed, preferably by direct gene transfer, e.g., by means of electroporation, with one or more DNA fragments (e.g., foreign DNA fragments).