Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: Provided herein are methods and compositions for modifying cells. Provided herein are methods and compositions for modifying an organism of a cell. Provided herein are methods and compositions for introducing polynucleotides and/or polypeptides into a nucleus of a cell.

Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.

Abstract: A fiber optic magnetic switch device comprising a fiber optic transceiver ving a light source and a length of fiber optic cable for transmitting light to a fiber optic magnetic switch. The switch includes a magnet, a position biased shutter operably attached to the magnet, a pair of SMA type connectors positioned to align the shutter there between, a repelling orientation magnet positioned proximate the shutter to orient the shutter in a first, secured repelling orientation to allow light through the shutter and further operable to prevent passage of light through the shutter in a second, unsecured condition. An alarm signals the failure of light to be transmitted along the fiber optic cable and through the shutter. Alternatively, the device may include a second fiber optic cable operable between the transceiver and the alarm during operation of the device and positioned to activate the alarm upon interruption of light transmission therein such as when it is tampered with.

Abstract: A friction clutch for use in a gear changing transmission for facilitating a power transmission from a rotating shaft of a working exhaust-gas turbine onto a crankshaft of an internal combustion engine, which friction clutch, depending on the engine and/or turbine speed, automatically closes in the direction of engagement and opens in the direction of disengagement, with the friction members of the clutch being able to be compressed in the direction of engagement against a spring force (cup springs) by a piston of a rotating cylinder-piston-unit provided on the drive side of the clutch, such that a fluid conveyed from an external pump into the cylinder applies a fluid pressure onto the piston in response to rotation (centrifugal force). The engine lubricant is preferably used as the fluid.