Abstract: A fluorescent ink composition comprising functionalized fluorescent nanocrystals, an aqueous-based ink carrier comprising water or a water-based solution, and a binder. Also provided are methods of providing an image or a security mark on a substrate for subsequent identification by applying a fluorescent ink composition onto the substrate.

Abstract: A diffusion controlling bioreactor that selectively controls the molecular diffusion between fluids through at least one microchannel in fluid communication with a reaction reservoir. Length and cross-sectional area of the microchannel may be selected to obtain a predetermined rate of molecular diffusion between fluids. When the fluids are liquids, flow through the microchannel is laminar and the capillary action of the microchannel and fluid is such that the fluid flow is regulated, and may have a structure configured to minimize the chances of fluid leakage from the bioreactor, even if the bioreactor is turned in various directions. In certain embodiments, one or more diffusion control chambers regulate fluid flow and diffusion between a reaction reservoir and an outside atmosphere.

Abstract: A fluorescent ink composition comprising functionalized fluorescent nanocrystals, an aqueous-based ink carrier comprising water or a water-based solution, and a binder. Also provided are methods of providing an image or a security mark on a substrate for subsequent identification by applying a fluorescent ink composition onto the substrate.

Abstract: A fluorescent ink composition comprising functionalized fluorescent nanocrystals, an aqueous-based ink carrier comprising water or a water-based solution, and a binder. Also provided are methods of providing an image or a security mark on a substrate for subsequent identification by applying a fluorescent ink composition onto the substrate.

Abstract: Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to operably link to a molecular probe, and secondary dots comprise a plurality of polynucleotide strands which are complementary to the plurality of polynucleotide strands of the primary dots. Also provided is a method for detecting the presence or absence of target molecule in a sample comprising operably linking primary dots to molecular probe, contacting the complex formed with the sample, contacting the sample with successive additions of secondary dots and primary dots. If target molecule is present in the sample, the primary dots and secondary dots will form dendrimers that can be detected by fluorescence emission.

Abstract: A diffusion controlling bioreactor that selectively controls the molecular diffusion between fluids through at least one microchannel in fluid communication with a reaction reservoir. Length and cross-sectional area of the microchannel may be selected to obtain a predetermined rate of molecular diffusion between fluids. When the fluids are liquids, flow through the microchannel is laminar and the capillary action of the microchannel and fluid is such that the fluid flow is regulated, and may have a structure configured to minimize the chances of fluid leakage from the bioreactor, even if the bioreactor is turned in various directions. In certain embodiments, one or more diffusion control chambers regulate fluid flow and diffusion between a reaction reservoir and an outside atmosphere.

Abstract: A diffusion controlling bioreactor that selectively controls the molecular diffusion between fluids through a microchannel in fluid communication with a reaction reservoir. The length and cross-sectional area are selected to obtain a predetermined rate of molecular diffusion between fluids. When the fluids are liquids, flow through the microchannel is laminar and the capillary action of the microchannel and fluid is such that the fluid does not flow into the reaction reservoir unless the pressure of the fluid is increased by an external source, thereby minimizing contamination of the bioreactor. The instant invention may also utilize at least one microchannel and reagent reservoir to regulate, rather than prevent, the passage of various molecules into the bioreactor. A pressure equalizing vent operating on similar principles to the microchannel may have a structure configured to minimize the chances of fluid leakage from the bioreactor, even if the bioreactor is turned in various directions.

Abstract: A bioreactor analysis system for incubating and analysis of a bioreactive material. The system comprises at least one bioreactor, preferably controlled environment bioreactors. The bioreactor may be held in a sleeve, and multiple sleeves may form a series that moving bioreactors into various storage and interventional positions. At least one interventional assembly interacts with the bioreactor while in the sleeve, and alternately, additional interventional assemblies may interact with the bioreactor while out of the sleeve. A jacket with an access port may surround the bioreactor, which may include a temperature management system. Alternately, a plurality of bioreactors may be joined to a storage array by the cooperation of intrinsic structures in the bioreactors and array. A control system allows for multiple individualized commands to be directed to any one or many of the bioreactors, and may utilize programs resident in the system or in remote locations far from the system.

Abstract: Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to operably link to a molecular probe, and secondary dots comprise a plurality of polynucleotide strands which are complementary to the plurality of polynucleotide strands of the primary dots. Also provided is a method for detecting the presence or absence of target molecule in a sample comprising operably linking primary dots to molecular probe, contacting the complex formed with the sample, contacting the sample with successive additions of secondary dots and primary dots. If target molecule is present in the sample, the primary dots and secondary dots will form dendrimers that can be detected by fluorescence emission.

Abstract: Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

Abstract: An identifiable code pattern, a method of providing a carrier material with an identifiable code pattern, and a method for detecting an identifiable code pattern are based on applying water-soluble nanocrystals to carrier materials in a spatially arranged format that can be detected by exposing the identifiable code pattern to an excitation light source in exciting the water-soluble nanocrystals, and detecting any fluorescence peaks emitted from the excited water-soluble nanocrystals using a detection system. Provided are compositions comprising water-soluble quantum dot compounds which may be used to apply to carrier materials in a method of forming an identifiable code pattern that may be used to provide discrete, detectable signals which may be detected for purposes of identification and/or verification.

Abstract: A diffusion controlling bioreactor that selectively controls the molecular diffusion between fluids through a microchannel in fluid communication with a reaction reservoir. The length and cross-sectional area are selected to obtain a predetermined rate of molecular diffusion between fluids. When the fluids are liquids, flow through the microchannel is laminar and the capillary action of the microchannel and fluid is such that the fluid does not flow into the reaction reservoir unless the pressure of the fluid is increased by an external source, thereby minimizing contamination of the bioreactor. The instant invention may also utilize at least one microchannel and reagent reservoir to regulate, rather than prevent, the passage of various molecules into the bioreactor. A pressure equalizing vent operating on similar principles to the microchannel may have a structure configured to minimize the chances of fluid leakage from the bioreactor, even if the bioreactor is turned in various directions.

Abstract: Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

Abstract: Provided are a functionalized, encapsulated fluorescent nanocrystal comprising a liposome having encapsulated therein one or more fluorescent nanocrystals; use of the functionalized, encapsulated fluorescent nanocrystals in detection systems; and a method of producing functionalized, encapsulated fluorescent nanocrystals. A method of using the functionalized encapsulated fluorescent nanocrystals having affinity molecule bound thereto comprises contacting the functionalized encapsulated fluorescent nanocrystals with a sample so that complexes are formed between the functionalized encapsulated fluorescent nanocrystals and substrate for which the affinity molecule has binding specificity, if the substrate is present; exposing the complexes in the detection system to an excitation light source, and detecting a fluorescence peak emitted from the complexes, if present.

Abstract: An identifiable code pattern, a method of providing a carrier material with an identifiable code pattern, and a method for detecting an identifiable code pattern are based on applying water-soluble nanocrystals to carrier materials in a spatially arranged format that can be detected by exposing the identifiable code pattern to an excitation light source in exciting the water-soluble nanocrystals, and detecting any fluorescence peaks emitted from the excited water-soluble nanocrystals using a detection system.

Abstract: A biological tissue regenerative agent and method for preparing the same. The agent comprises the compounds that are usually found sequestered within platelets, along with platelet cytosolic components, and serum. The agent is prepared by preparing two quanta of blood. The first is clotted, the cells discarded and the serum retained. The second quantum undergoes concentration and lysis of the platelets therein, followed by recombination of the lysed platelets and platelet internal products with serum to form the agent. In a preferred embodiment, lysis of platelets is accomplished by providing an effective amount of calcium. The agent may be further purified and may be frozen or freeze dried for storage.

Abstract: A biological tissue regenerative agent and method for preparing the same. The agent comprises the compounds that are usually found sequestered within platelets, along with platelet cytosolic components, and serum. The agent is prepared by preparing two quanta of blood. The first is clotted, the cells discarded and the serum retained. The second quantum undergoes concentration and lysis of the platelets therein, followed by recombination of the lysed platelets and platelet internal products with serum to form the agent. In a preferred embodiment, lysis of platelets is accomplished by providing an effective amount of calcium. The avoidance of a platelet release reaction and the presence of alpha-2 macroglobulin in the serum suppresses active TGF-? in the agent. The agent may be further purified and may be frozen or freeze dried for storage.

Abstract: Provided are vaccines, methods of making the vaccines and methods for administering the vaccines for immunotherapy of an individual bearing, or at risk for developing, solid nonlymphoid tumor. The vaccine comprises an immunotherapeutic composition and tumor-associated antigen, and may further comprise one or more of an immunomodulator or a pharmaceutically acceptable carrier. A method of immunotherapy of an individual comprises administering to the individual an amount of the vaccine effective to suppress a TH2 response, and to induce a TH1 response against solid nonlymphoid tumor, in an individual having a TH2/TH1 imbalance.

Abstract: A method for screening an individual for a pathological condition comprising a pro-tumor immune response by assaying a clinical sample, obtained from the individual, with a plurality of affinity ligands for detecting and determining an amount of mononuclear cell phenotype. The amount of mononuclear cell phenotype determined in the clinical sample is then compared to a reference value for the mononuclear cell phenotype, wherein a difference in the amount of mononuclear cell phenotype determined from the clinical sample as compared to the reference value comprises an indicator of the presence of a pro-tumor immune response.