Patents by Inventor Enbo Ma
Enbo Ma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220315914Abstract: The present disclosure provides variant type V CRISPR/Cas effector polypeptides, nucleic acids encoding the variant polypeptides, and systems comprising the variant polypeptides or nucleic acids encoding same. The present disclosure provides methods for modifying a target nucleic acid, using a variant polypeptide of the present disclosure.Type: ApplicationFiled: July 6, 2020Publication date: October 6, 2022Inventors: Jennifer A. Doudna, Enbo Ma, Junjie Liu
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Patent number: 11447824Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: GrantFiled: July 13, 2020Date of Patent: September 20, 2022Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Publication number: 20220025353Abstract: The present disclosure provides devices and methods for delivering a biomolecule into a cell. A delivery device of the present disclosure includes a first reservoir, a second reservoir, a porous membrane comprising a nanopore, and two or more electrodes configured to generate an electric field across the porous membrane for delivery of a biomolecule present in the second reservoir through the nanopore of the porous membrane and into a cell present in the first reservoir.Type: ApplicationFiled: September 26, 2019Publication date: January 27, 2022Inventors: Jennifer A. Doudna, Yuhong Cao, Enbo Ma, Peidong Yang
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Publication number: 20210388437Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: ApplicationFiled: July 29, 2021Publication date: December 16, 2021Inventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Patent number: 11118224Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: GrantFiled: June 9, 2020Date of Patent: September 14, 2021Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Publication number: 20200399697Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: ApplicationFiled: July 13, 2020Publication date: December 24, 2020Inventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Publication number: 20200299768Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: ApplicationFiled: June 9, 2020Publication date: September 24, 2020Inventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Publication number: 20190241954Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: ApplicationFiled: January 30, 2019Publication date: August 8, 2019Inventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Patent number: 10253365Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.Type: GrantFiled: February 14, 2018Date of Patent: April 9, 2019Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Janice S. Chen, Lucas Benjamin Harrington, Enbo Ma
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Patent number: 9434930Abstract: The present disclosure provides a method for producing a Dicer polypeptide in a prokaryotic host cell. The present disclosure further provides a purified Dicer complex. The present disclosure further provides kits for producing a Dicer polypeptide in a prokaryotic host cell.Type: GrantFiled: August 25, 2014Date of Patent: September 6, 2016Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Jennifer A. Doudna, Enbo Ma
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Publication number: 20150361406Abstract: The present disclosure provides a method for producing a Dicer polypeptide in a prokaryotic host cell. The present disclosure further provides a purified Dicer complex. The present disclosure further provides kits for producing a Dicer polypeptide in a prokaryotic host cell.Type: ApplicationFiled: August 25, 2014Publication date: December 17, 2015Inventors: Jennifer A. Doudna, Enbo Ma
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Patent number: 8852911Abstract: The present disclosure provides a method for producing a Dicer polypeptide in a prokaryotic host cell. The present disclosure further provides a purified Dicer complex. The present disclosure further provides kits for producing a Dicer polypeptide in a prokaryotic host cell.Type: GrantFiled: August 2, 2012Date of Patent: October 7, 2014Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Enbo Ma
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Publication number: 20130196383Abstract: The present disclosure provides a method for producing a Dicer polypeptide in a prokaryotic host cell. The present disclosure further provides a purified Dicer complex. The present disclosure further provides kits for producing a Dicer polypeptide in a prokaryotic host cell.Type: ApplicationFiled: August 2, 2012Publication date: August 1, 2013Inventors: Jennifer A. Doudna, Enbo Ma
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Patent number: 8440430Abstract: A modified Dicer polypeptide is provided, which modified Dicer polypeptide exhibits enhanced catalytic activity. Also provided is a method for producing small regulatory RNAs from a dsRNA, involving contacting a dsRNA with a subject modified Dicer. Small regulatory RNAs produced by a subject method find use in a variety of applications, including research and therapeutic applications.Type: GrantFiled: March 18, 2009Date of Patent: May 14, 2013Assignee: The Regents of the University of CaliforniaInventors: Jennifer Doudna, Enbo Ma, Ian J. MacRae
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Publication number: 20110117610Abstract: A modified Dicer polypeptide is provided, which modified Dicer polypeptide exhibits enhanced catalytic activity. Also provided is a method for producing small regulatory RNAs from a dsRNA, involving contacting a dsRNA with a subject modified Dicer. Small regulatory RNAs produced by a subject method find use in a variety of applications, including research and therapeutic applications.Type: ApplicationFiled: March 18, 2009Publication date: May 19, 2011Inventors: Jennifer Doudna, Enbo Ma, Ian J. Macrae