Abstract: Some embodiments of the present disclosure relate to a method and system where a fiber optic probe is obtained. In some embodiments, the fiber optic probe comprises a distal end. In some embodiments, the fiber optic probe is introduced between an outer surface of an eye and an anterior chamber of an eye. In some embodiments, the fiber optic probe is advanced into one or more portions of the eye. In some embodiments, a plurality of pulses of laser radiation are delivered through a laser and into the eye. In some embodiments, the laser is disposed at a distal end of the fiber optic probe. In some embodiments, ocular tissue of the eye is ablated with the plurality of pulses of laser radiation. In some embodiments, the ablating generates a drainage channel that extends from the anterior chamber of the eye to the subconjunctival space of the eye.

Abstract: A device for guiding a cutting blade during surgical manipulation of the sclera and a system including a cutting blade and a device for guiding the cutting blade during surgical manipulation of the sclera. Also, a method for using a depth-control cutting blade and a device for guiding the cutting blade to perform surgical manipulation of the sclera in a desired shape, size, and thickness.

Abstract: A stent manufacturing assembly for assisting in the manufacturing of a medical stent and a process for manufacturing a medical stent are disclosed. A patterned sheet of metal can be wrapped around the manufacturing assembly's outer surface. The assembly includes a mandrel and a sleeve. The mandrel includes a rigid and substantially cylindrical external surface, and the sleeve surrounds the mandrel and has a variable inner diameter. The sleeve adheres to the inner surface of the stent formed around the sleeve to allow the sleeve to remain in place. After the mandrel is slidably removed from the sleeve, the sleeve radially collapses and contracts, thereby causing minimal shear stress on the stent's inner surface and preventing or minimizing friction and pressure between the mandrel and the stent.

Abstract: An imaging apparatus with an autofocus mechanism for obtaining focused images. The apparatus comprises: an objective lens, a focus controller for altering a distance between the objective lens and a sample, an object finder for finding objects of interest within the sample, for example cells, and a light intensity measurement unit which measures light intensity levels of the thus identified objects of interest. The focus control alters the sample-objective distance to maximize the light intensity levels being measured, thereby to arrive at a focus position. Objects of interest may be identified by filtering out large objects and optionally by masking out background regions. The apparatus is useful for microscopy and particularly for fluorescent imaging in which light levels are low and noise is often high.

Abstract: A data acquisition and display system comprises: a data acquisition device, such as a camera, microscope, telescope, thermal imager, medical scanning device, radar etc, operable to acquire data in scans over a field of interest using each of at least a first and a second data acquisition method. A field data storage device stores the field data together with corresponding field location data that describes a location within the field of interest. A field data display device such as a screen is operable to display simultaneously data from matched locations in the field of interest acquired respectively by the different data acquisition methods. The data, generally image data, may be superimposed or displayed side by side as desired. Preferably, one of the images being displayed is live whilst the other is recalled from the field data storage device.

Abstract: An imaging apparatus with an autofocus mechanism for obtaining focused images. The apparatus comprises: an objective lens, a focus controller for altering a distance between the objective lens and a sample, an object finder for finding objects of interest within the sample, for example cells, and a light intensity measurement unit which measures light intensity levels of the thus identified objects of interest. The focus control alters the sample-objective distance to maximize the light intensity levels being measured, thereby to arrive at a focus position. Objects of interest may be identified by filtering out large objects and optionally by masking out background regions. The apparatus is useful for microscopy and particularly for fluorescent imaging in which light levels are low and noise is often high.

Abstract: A method of preparing nucleated peripheral blood or bone marrow cells optimized for at least dual mode imaging. The method including: (a) isolating nucleated cells from a peripheral blood or a bone marrow sample; and (b) resuspending the nucleated cells in the presence of a morphology preserver including at least 1% serum, and recovering a cell fraction thereby preparing the nucleated peripheral blood or bone marrow cells optimized for at least dual mode imaging.

Abstract: The invention provides methods of detecting cancerous cells in biological samples using a double staining/dual imaging approach, which can be used to diagnose cancer. More specifically, the present invention provides methods of diagnosing bladder cancer by a simultaneous scanning of cell morphology and FISH signals of cells derived from a urine sample.

Abstract: A data acquisition and display system comprises: a data acquisition device, such as a camera, microscope, telescope, thermal imager, medical scanning device, radar etc, operable to acquire data in scans over a field of interest using each of at least a first and a second data acquisition method. A field data storage device stores the field data together with corresponding field location data that describes a location within the field of interest. A field data display device such as a screen is operable to display simultaneously data from matched locations in the field of interest acquired respectively by the different data acquisition methods. The data, generally image data, may be superimposed or displayed side by side as desired. Preferably, one of the images being displayed is live whilst the other is recalled from the field data storage device.

Abstract: A method of mapping areas of a slide includes the steps of selectively illuminating the slide from a first light source oriented generally obliquely to the surface of the slide, obtaining a first image of the slide illuminated by the first light source, selectively illuminated the slide from a second light source providing generally scattered light, obtaining an image of said second image illuminated by the second light source, and generating a map of areas of significance based on the first and second images.

Abstract: The MTF for an optical or electronic device(s) is evaluated over a substantial portion of an image signal. As a result, a composite MTF representing a more global evaluation is achieved. Furthermore, the MTF for all or part of a system can be evaluated in situ.