Patents by Inventor Eric V. Anslyn
Eric V. Anslyn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240124910Abstract: Disclosed are methods, systems, components, and compositions for synthesis of sequence defined polymers. The methods, systems, components, and compositions may be utilized for incorporating novel substrates that include non-standard amino acid monomers and non-amino acid monomers into sequence defined polymers. As disclosed herein, the novel substrates may be utilized for acylation of tRNA via flexizyme catalyzed reactions. The tRNAs thus acylated with the novel substrates may be utilized in synthesis platforms for incorporating the novel substrates into a sequence defined polymer.Type: ApplicationFiled: February 2, 2022Publication date: April 18, 2024Inventors: Michael Christopher Jewett, Joongoo Lee, Eric V. Anslyn, Jaime Coronado, Jongdoo Lim
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Publication number: 20240002925Abstract: Provided herein are compositions, systems, methods, and kits for peptide analysis, including peptide sequencing. Aspects of the present disclosure provide bifunctional reagents which may selectively couple to amino acids and selectively couple to detectable species. Aspects of the present disclosure further provide methods for using these bifunctional reagents to sequence and analyze peptides.Type: ApplicationFiled: November 18, 2022Publication date: January 4, 2024Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Eric V. ANSLYN, Edward MARCOTTE, Cecil J. HOWARD, II, Jagannath SWAMINATHAN, Angela M. BARDO, Brendan FLOYD, Brandon HOSFORD, Le ZHANG, Emily Faith BABCOCK, Caroline M. HINSON
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Publication number: 20230107647Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: July 7, 2022Publication date: April 6, 2023Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN
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Publication number: 20230076975Abstract: Described herein are methods for selectively cleaving the C-terminal amino acid of a peptide or protein. The methods described herein may be applicable for, for example, single-molecule peptide or protein sequencing.Type: ApplicationFiled: August 18, 2022Publication date: March 9, 2023Inventors: Eric V. ANSLYN, Edward MARCOTTE, Cecil J. HOWARD, II, Jagannath SWAMINATHAN, Angela M. BARDO, Caroline M. HINSON, Brendan FLOYD, Le ZHANG
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Patent number: 11435358Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: GrantFiled: October 1, 2021Date of Patent: September 6, 2022Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan
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Publication number: 20220091130Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: October 1, 2021Publication date: March 24, 2022Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
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Patent number: 11162952Abstract: The disclosure concerns methods for identifying amino acids in peptides, including peptides including unnatural amino acids. Various aspects of the present disclosure provide compositions and methods for amino acid-type specific labeling, as well as methods for detecting the labels. Further disclosed herein are strategies for highly multiplexed, high-throughput peptide analysis.Type: GrantFiled: September 16, 2019Date of Patent: November 2, 2021Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
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Patent number: 11105812Abstract: The present invention relates to methods for identifying amino acids in peptides. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: GrantFiled: March 16, 2017Date of Patent: August 31, 2021Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Jagannath Swaminathan, Andrew Ellington, Eric V. Anslyn
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Publication number: 20210072251Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: December 10, 2019Publication date: March 11, 2021Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
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Publication number: 20200124613Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: December 10, 2019Publication date: April 23, 2020Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
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Patent number: 10545153Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides with one or more unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: GrantFiled: September 15, 2015Date of Patent: January 28, 2020Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
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Publication number: 20200018768Abstract: Identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level is an unmet challenge in the field of protein sequencing. Herein are methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the N-terminal amino acid is labeled with a first label and an internal amino acid is labeled with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: September 16, 2019Publication date: January 16, 2020Inventors: Edward MARCOTTE, Eric V. ANSLYN, Andrew ELLINGTON, Jagannath SWAMINATHAN, Erik HERNANDEZ, Amber JOHNSON, Alexander BOULGAKOV, James L. BACHMAN, Helen SEIFERT
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Publication number: 20170276686Abstract: The present invention relates to the field of identifying proteins and peptides, and more specifically large-scale sequencing of single peptides in a mixture of diverse peptides at the single molecule level. The present invention also relates to methods for identifying amino acids in peptides, including peptides comprising unnatural amino acids. In one embodiment, the present invention contemplates labeling the N-terminal amino acid with a first label and labeling an internal amino acid with a second label. In some embodiments, the labels are fluorescent labels. In other embodiments, the internal amino acid is Lysine. In other embodiments, amino acids in peptides are identified based on the fluorescent signature for each peptide at the single molecule level.Type: ApplicationFiled: September 15, 2015Publication date: September 28, 2017Inventors: Edward Marcotte, Eric V. Anslyn, Andrew Ellington, Jagannath Swaminathan, Erik Hernandez, Amber Johnson, Alexander Boulgakov, James L. Bachman, Helen Seifert
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Patent number: 9116147Abstract: Compositions, methods, and systems for detecting small molecules using chemiluminescent signaling assay technology are provided. One system provided herein comprises a chromophore; an oxalate ester, a peroxide, and a modulating agent, wherein the modulating agent will perturb a chemiluminescent signal generated by an interaction among the chromophore, the oxalate ester, and a peroxide; and the perturbation will occur in response to an analyte. One method provided herein comprises combining a chromophore, an oxalate ester, a peroxide, and a modulating agent, wherein: the modulating agent will perturb a chemiluminescent signal generate by an interaction among the chromophore, the oxalate ester, and a peroxide; and the perturbation will occur in response to an analyte. Another method provides a colorimetric or fluorometric signal response in the presence of an analyte.Type: GrantFiled: March 2, 2009Date of Patent: August 25, 2015Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Eric V. Anslyn, Himali Hewage, Ronald Houk, Damon V. Borich, Robert E. Hanes, Jr., Jason A. Neeser
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Publication number: 20140179014Abstract: Nitric oxide probes including a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided.Type: ApplicationFiled: December 20, 2013Publication date: June 26, 2014Inventors: Eric V. Anslyn, Youjun Yang, Michelle M. Adams
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Publication number: 20140120574Abstract: Nitric oxide probes comprising a compound represented by Formula I are provided. Methods of using this nitric oxide probes to detect nitric oxide are also provided.Type: ApplicationFiled: June 4, 2013Publication date: May 1, 2014Inventors: Eric V. Anslyn, Youjun Yang, Michelle A. Ivy
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Patent number: 8637323Abstract: Nitric oxide probes including a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided.Type: GrantFiled: October 4, 2012Date of Patent: January 28, 2014Assignee: Board of Regents, The University of Texas SystemInventors: Eric V. Anslyn, Youjun Yang, Michelle M. Adams
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Publication number: 20130302903Abstract: Nitric oxide probes comprising a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided.Type: ApplicationFiled: October 4, 2012Publication date: November 14, 2013Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventors: Eric V. Anslyn, Youjun Yang, Michelle M. Adams
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Patent number: 8377712Abstract: Compositions for detection of chemical warfare agents that comprise oximate anion reactive sites and fluorophore cores. Methods for detecting a chemical warfare agents that comprise providing a detector molecule comprising an oximate anion reactive site and a fluorophore core and detecting fluorescence from the detector molecule. Methods for enhancing the reactivity of an oximate nucleophile that comprise introducing an oxime into an aprotic solvent and deprotonating the oxime to form the oximate nucleophile with a base that creates noncoordinating anions.Type: GrantFiled: December 11, 2006Date of Patent: February 19, 2013Assignee: Board of Regents, The University of Texas SystemInventors: Karl J. Wallace, Eric V. Anslyn
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Patent number: 8257967Abstract: A system for the rapid characterization of multi-cardiovascular risk factor analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member, in which a plurality of cavities may be formed. A series of chemically sensitive particles, in one embodiment, are positioned within the cavities. The particles may produce a signal when a receptor, coupled to the particle, interacts with the cardiovascular risk factor analyte and the particle-analyte complex is visualized using a visualization reagent. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized. In an embodiment, each cavity of the plurality of cavities is designed to capture and contain a specific size particle. Flexible projections may be positioned over each of the cavities to provide retention of the particles in the cavities.Type: GrantFiled: April 28, 2003Date of Patent: September 4, 2012Assignee: Board of Regents, The University of Texas SystemInventors: John T. McDevitt, Eric V. Anslyn, Jason B. Shear, Dean P. Neikirk, Nick J. Christodoulides