Patents by Inventor Erik Leeming Kvam
Erik Leeming Kvam has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230219080Abstract: A closed system centrifuge tube is described and discussed herein that enables removal of waste and transfer and removal of samples via one or more ports of the centrifuge tube within the closed system, without exposing the sample to the outer environment. This may enable greater sample retention during sample processing and centrifugation of the sample.Type: ApplicationFiled: January 11, 2022Publication date: July 13, 2023Inventors: Christopher Michael Puleo, Ralf Lenigk, Tejas Suresh Khire, Tyler Hammond, Erik Leeming Kvam, Allison Caron
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Patent number: 11597960Abstract: The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.Type: GrantFiled: July 1, 2019Date of Patent: March 7, 2023Assignee: General Electric CompanyInventors: Alex David Corwin, Erik Leeming Kvam, Christine Lynne Surrette, Brian Joseph Scherer, Pei-Hsin Kuo, Ralf Lenigk
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Patent number: 11590502Abstract: The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.Type: GrantFiled: July 1, 2019Date of Patent: February 28, 2023Assignee: General Electric CompanyInventors: Alex David Corwin, Erik Leeming Kvam, Christine Lynne Surrette, Brian Joseph Scherer, Pei-Hsin Kuo, Ralf Lenigk, Tyler John Hammond
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Publication number: 20220389473Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.Type: ApplicationFiled: July 19, 2022Publication date: December 8, 2022Inventors: John Richard Nelson, Robert Scott Duthie, Erik Leeming Kvam, Wei Gao
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Patent number: 11421259Abstract: Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.Type: GrantFiled: July 20, 2018Date of Patent: August 23, 2022Assignee: Global Life Sciences Solutions Operations UK LtdInventors: John Richard Nelson, Robert Scott Duthie, Erik Leeming Kvam, Wei Gao
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Publication number: 20220243243Abstract: Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.Type: ApplicationFiled: June 9, 2020Publication date: August 4, 2022Inventors: Brian Michael Davis, Erik Leeming Kvam, John Richard Nelson, Lisa A. Lowery, Wei Gao
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Publication number: 20220041976Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.Type: ApplicationFiled: August 30, 2019Publication date: February 10, 2022Inventors: Matthew Jeremiah Misner, Rachel Marie Gettings, Christine Angela Morton, Erik Leeming Kvam, Jason William CASTLE, Anindya Kanti De, Jared Timothy Hale, Craig Patrick Galligan, Vincent Francis Pizzi
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Patent number: 11142758Abstract: Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.Type: GrantFiled: March 20, 2017Date of Patent: October 12, 2021Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Erik Leeming Kvam, John Richard Nelson, Gregory Andrew Grossmann, Ryan Charles Heller, Erin Jean Finehout, Christopher Michael Puleo, William Patrick Waters
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Patent number: 11028359Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.Type: GrantFiled: September 11, 2018Date of Patent: June 8, 2021Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLCInventors: Matthew Jeremiah Misner, Rachel Marie Gettings, Christine Angela Morton, Erik Leeming Kvam, Jason William Castle, Anindya Kanti De, Jared Timothy Hale, Craig Patrick Galligan, Vincent Francis Pizzi
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Patent number: 11001868Abstract: Methods for in vitro transcription and translation using a double-stranded concatemeric DNA in a eukaryotic cell-free expression system are provided. The method includes the steps of (a) contacting a double-stranded concatemeric DNA with a eukaryotic cell-free expression system, and (b) expressing a protein in vitro from the double-stranded concatemeric DNA in the eukaryotic cell-free expression system. The double-stranded concatemeric DNA includes a plurality of tandem repeat sequences. The plurality of tandem repeat sequences includes an expression sequence including a promoter, a cap-independent translation element (CITE), and an open reading frame. A final concentration of the double-stranded concatemeric DNA in the eukaryotic cell-free expression system is in a range from about 0.1 ng/?L to about 35 ng/?L. A RCA product DNA may be used as the double stranded concatemer DNA for the methods.Type: GrantFiled: August 11, 2017Date of Patent: May 11, 2021Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Erik Leeming Kvam, John Richard Nelson, Wei Gao
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Patent number: 10960090Abstract: A general illumination lighting system and/or at least one targeted surface illumination lighting system. The general illumination lighting system including a general illuminating light source configured to generate light toward one or more surfaces or materials to inactivate one or more pathogens on the one or more surfaces or materials. The at least one targeted surface illumination lighting system including one or more targeted surface illuminating light sources that are integrated into or coupled to a plurality of devices, equipment, or fixtures. The one or more targeted surface illuminating light sources configured to generate targeted light toward one or more surfaces of the devices, equipment, or fixtures to inactivate one or more pathogens on the one or more surfaces. The light from the general illuminating light source and the one or more targeted surface illuminating light source including an inactivating portion having wavelengths in a range of 280 to 380 nanometers.Type: GrantFiled: May 25, 2018Date of Patent: March 30, 2021Assignee: CURRENT LIGHTING SOLUTIONS, LLCInventors: Thomas Clynne, Gary Robert Allen, Kevin Jeffrey Benner, Kevin James Vick, Erik Leeming Kvam, Jonathan Alan Murray
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Publication number: 20210001336Abstract: The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.Type: ApplicationFiled: July 1, 2019Publication date: January 7, 2021Inventors: Alex David Corwin, Erik Leeming Kvam, Christine Lynne Surrette, Brian Joseph Scherer, Pei-Hsin Kuo, Ralf Lenigk, Tyler John Hammond
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Publication number: 20210002688Abstract: The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.Type: ApplicationFiled: July 1, 2019Publication date: January 7, 2021Inventors: Alex David Corwin, Erik Leeming Kvam, Christine Lynne Surrette, Brian Joseph Scherer, Pei-Hsin Kuo, Ralf Lenigk
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Publication number: 20200392554Abstract: Provided are techniques for generating expression products using one or more nucleic acid concatemers that include tandem repeats of a nucleic acid sequence encoding the expression product or products. In one embodiment, different expression products may be co-expressed using a concatemer mixture of a first nucleic acid concatemer and a second nucleic acid concatemer having a predefined ratio to one another.Type: ApplicationFiled: June 13, 2019Publication date: December 17, 2020Inventors: Brian Michael Davis, Erik Leeming Kvam, John Richard Nelson, Lisa Anne Lowery, Wei Gao
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Patent number: 10787695Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.Type: GrantFiled: June 1, 2017Date of Patent: September 29, 2020Assignee: General Electric CompanyInventors: Christopher Michael Puleo, Christine Lynne Surrette, Erik Leeming Kvam, Steven Yuehin Go, Feng Chen, John Richard Nelson, Craig Patrick Galligan, Radislav Alexandrovich Potyrailo, Gregory Andrew Grossmann
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Patent number: 10655167Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.Type: GrantFiled: April 9, 2018Date of Patent: May 19, 2020Assignee: General Electric CompanyInventors: Ryan Charles Heller, Erik Leeming Kvam, John Richard Nelson
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Patent number: 10625242Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.Type: GrantFiled: October 5, 2016Date of Patent: April 21, 2020Assignee: General Electric CompanyInventors: Ernest William Kovacs, Frank John Mondello, Erik Leeming Kvam, Bing Li
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Publication number: 20200080047Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.Type: ApplicationFiled: September 11, 2018Publication date: March 12, 2020Inventors: Matthew Jeremiah Misner, Rachel Marie Gettings, Christine Angela Morton, Erik Leeming Kvam, Jason William Castle, Anindya Kanti De, Jared Timothy Hale, Craig Patrick Galligan, Vincent Francis Pizzi
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Patent number: 10518196Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.Type: GrantFiled: July 13, 2016Date of Patent: December 31, 2019Assignee: General Electric CompanyInventors: Christopher Michael Puleo, Craig Patrick Galligan, Gregory Andrew Grossman, Erik Leeming Kvam, Robert Scott Duthie, Kenneth Wayne Rigby, Paul Michael Smigelski, Jr., Victoria Eugenia Cotero, Jason William Castle, John Donald Burczak, James Edward Rothman
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Patent number: 10472620Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.Type: GrantFiled: July 1, 2014Date of Patent: November 12, 2019Assignee: General Electric CompanyInventors: John Richard Nelson, David Roger Moore, Robert Scott Duthie, Matthew Jeremiah Misner, Gregory Andrew Grossmann, Elizabeth Marie Dees, Patrick McCoy Spooner, Erik Leeming Kvam, Andrew Arthur Paul Burns, Vicki Herzl Watkins