Patents by Inventor Esther Musgrave-Brown
Esther Musgrave-Brown has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230340568Abstract: The present disclosure relates to methods, compositions, and kits for generating a library of tagged nucleic acid fragments without using PCR amplification, including methods and compositions for fragmenting and tagging nucleic acids (e.g., DNA) using transposome complexes immobilized on solid support.Type: ApplicationFiled: May 12, 2023Publication date: October 26, 2023Applicant: Illumina Cambridge LimitedInventors: Andrew Slatter, Esther Musgrave-Brown, Susan C. Verity, Niall Anthony Gormley
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Patent number: 11685946Abstract: The present disclosure relates to methods, compositions, and kits for generating a library of tagged nucleic acid fragments without using PCR amplification, including methods and compositions for fragmenting and tagging nucleic acids (e.g., DNA) using transposome complexes immobilized on solid support.Type: GrantFiled: January 10, 2020Date of Patent: June 27, 2023Inventors: Andrew Slatter, Esther Musgrave-Brown, Susan C. Verity, Niall Anthony Gormley
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Patent number: 11306348Abstract: The present disclosure relates to methods, compositions, and kits for generating a library of tagged nucleic acid fragments without using PCR amplification, including methods and compositions for fragmenting and tagging nucleic acids (e.g., DNA) using transposome complexes immobilized on solid support.Type: GrantFiled: May 3, 2021Date of Patent: April 19, 2022Assignee: Illumina Cambridge LimitedInventors: Andrew Slatter, Esther Musgrave-Brown, Susan C. Verity, Niall Anthony Gormley
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Publication number: 20220033885Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.Type: ApplicationFiled: August 5, 2021Publication date: February 3, 2022Inventors: Robert OSBORNE, Esther Musgrave-Brown
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Publication number: 20210380972Abstract: The present disclosure is concerned with compositions, methods, and kits for preparing a sequencing library. In one embodiment, methods include producing a library of target nucleic acids having the same adapter at each end and then switching the identity of one adapter to result in target nucleic acids flanked by distinct adapters.Type: ApplicationFiled: June 10, 2021Publication date: December 9, 2021Applicants: Illumina, Inc., Illumina Cambridge Limited, Oregon Health & Science UniversityInventors: Andrew C. Adey, Ryan Mulqueen, Frank Steemers, Dmitry K Pokholok, Fan Zhang, Esther Musgrave-Brown
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Patent number: 11111524Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.Type: GrantFiled: November 22, 2019Date of Patent: September 7, 2021Assignee: OXFORD NANOPORE TECHNOLOGIES, LTD.Inventors: Robert Osborne, Esther Musgrave-Brown
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Publication number: 20210269856Abstract: The present disclosure relates to methods, compositions, and kits for generating a library of tagged nucleic acid fragments without using PCR amplification, including methods and compositions for fragmenting and tagging nucleic acids (e.g., DNA) using transposome complexes immobilized on solid support.Type: ApplicationFiled: May 3, 2021Publication date: September 2, 2021Applicant: ILLUMINA CAMBRIDGE LIMITEDInventors: Andrew Slatter, Esther Musgrave-Brown, Susan C. Verity, Niall Anthony Gormley
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Patent number: 10927408Abstract: A method of evaluating a sequence variation in a sample is provided. In some embodiments, the method may involve: amplifying a nucleic acid product from an initial sample; fragmenting an amount of the nucleic acid product to produce fragments; attaching an adaptor to each end of the fragments to produce adaptor-tagged fragments; sampling no more than 10% of the tagged fragments and amplifying them; sequencing at least some of the copies of the fragments to produce a plurality of sequence reads; grouping sequence reads for copies of fragments that have the same fragmentation breakpoints; deriving a consensus sequence for each of the read groups; and aligning the consensus sequences with a reference sequence.Type: GrantFiled: November 26, 2014Date of Patent: February 23, 2021Assignee: Personal Genome Diagnostics, Inc.Inventors: Robert Osborne, Esther Musgrave-Brown
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Publication number: 20200224246Abstract: The present disclosure relates to methods, compositions, and kits for generating a library of tagged nucleic acid fragments without using PCR amplification, including methods and compositions for fragmenting and tagging nucleic acids (e.g., DNA) using transposome complexes immobilized on solid support.Type: ApplicationFiled: January 10, 2020Publication date: July 16, 2020Applicant: ILLUMINA CAMBRIDGE LIMITEDInventors: Andrew Slatter, Esther Musgrave-Brown, Susan C. Verity, Niall Anthony Gormley
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Publication number: 20200190563Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.Type: ApplicationFiled: November 22, 2019Publication date: June 18, 2020Inventors: Robert OSBORNE, Esther Musgrave-Brown
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Patent number: 10626441Abstract: Described herein is a method for adding a counter sequence to the individual polynucleotide molecules of an initial nucleic acid sample. After addition of the counter sequence, the sample may be amplified and the number of initial target molecules in the sample can be estimated by counting the number of counter sequences associated with the amplified target molecules.Type: GrantFiled: April 27, 2017Date of Patent: April 21, 2020Assignee: Agilent Technologies, Inc.Inventors: Robert Osborne, James Casbon, Andreas Claas, Gi Mikawa, Esther Musgrave-Brown
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Patent number: 10519488Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.Type: GrantFiled: September 1, 2016Date of Patent: December 31, 2019Assignee: OXFORD NANOPORE TECHNOLOGIES, LTD.Inventors: Robert Osborne, Esther Musgrave-Brown
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Patent number: 10472667Abstract: Providing herein, among other things, is a method for preparing a nucleic acid for sequencing. In some embodiments, the method comprises a) amplifying a nucleic acid template using a dNTP mix that contains 5-methyl dCTP, thereby producing product nucleic acid molecules that contains methylcytosines; b) digesting the product nucleic acid molecules with a methylation-dependent restriction endonuclease, thereby cleaving the product nucleic acid molecules at sites that are adjacent to at least some of the methylcytosine and producing fragments of the product nucleic acid molecules; and c) ligating double-stranded adaptors onto the ends of the fragments to produce adaptor-ligated products.Type: GrantFiled: February 9, 2018Date of Patent: November 12, 2019Assignee: Agency for Science, Technology and ResearchInventors: Robert Osborne, Samuel Woodhouse, Esther Musgrave-Brown
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Publication number: 20180305737Abstract: Providing herein, among other things, is a method for preparing a nucleic acid for sequencing. In some embodiments, the method comprises a) amplifying a nucleic acid template using a dNTP mix that contains 5-methyl dCTP, thereby producing product nucleic acid molecules that contains methylcytosines; b) digesting the product nucleic acid molecules with a methylation-dependent restriction endonuclease, thereby cleaving the product nucleic acid molecules at sites that are adjacent to at least some of the methylcytosine and producing fragments of the product nucleic acid molecules; and c) ligating double-stranded adaptors onto the ends of the fragments to produce adaptor-ligated products.Type: ApplicationFiled: February 9, 2018Publication date: October 25, 2018Inventors: Robert Osborne, Samuel Woodhouse, Esther Musgrave-Brown
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Publication number: 20180237837Abstract: Described herein, among other things, is a method of sequencing, comprising: concatenating a plurality of fragments of genomic DNA to produce concatenated DNA; sequencing the concatenated DNA to produce a plurality of sequence reads, wherein at least some of the sequence reads comprise: at least the sequence of the 3? and/or 5? ends of a fragment that corresponds to the locus of interest and sequence of one or both of the fragments that flank the fragment in the concatenated DNA; and grouping the sequence reads that corresponds to the locus of interest using, for each of the grouped sequence reads: the 3? and/or 5? end sequences; and/or the flanking sequence.Type: ApplicationFiled: September 1, 2016Publication date: August 23, 2018Inventors: Robert OSBORNE, Esther Musgrave-Brown
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Patent number: 9920355Abstract: Providing herein, among other things, is a method for preparing a nucleic acid for sequencing. In some embodiments, the method comprises a) amplifying a nucleic acid template using a dNTP mix that contains 5-methyl dCTP, thereby producing product nucleic acid molecules that contains methylcytosines; b) digesting the product nucleic acid molecules with a methylation-dependent restriction endonuclease, thereby cleaving the product nucleic acid molecules at sites that are adjacent to at least some of the methylcytosine and producing fragments of the product nucleic acid molecules; and c) ligating double-stranded adaptors onto the ends of the fragments to produce adaptor-ligated products.Type: GrantFiled: November 12, 2015Date of Patent: March 20, 2018Assignee: POPULATION GENETICS TECHNOLOGIES LTD.Inventors: Robert Osborne, Samuel Woodhouse, Esther Musgrave-Brown
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Publication number: 20170335371Abstract: Providing herein, among other things, is a method for preparing a nucleic acid for sequencing. In some embodiments, the method comprises a) amplifying a nucleic acid template using a dNTP mix that contains 5-methyl dCTP, thereby producing product nucleic acid molecules that contains methylcytosines; b) digesting the product nucleic acid molecules with a methylation-dependent restriction endonuclease, thereby cleaving the product nucleic acid molecules at sites that are adjacent to at least some of the methylcytosine and producing fragments of the product nucleic acid molecules; and c) ligating double-stranded adaptors onto the ends of the fragments to produce adaptor-ligated products.Type: ApplicationFiled: November 12, 2015Publication date: November 23, 2017Inventors: Robert Osborne, Samuel Woodhouse, Esther Musgrave-Brown
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Publication number: 20170226562Abstract: Described herein is a method for adding a counter sequence to the individual polynucleotide molecules of an initial nucleic acid sample. After addition of the counter sequence, the sample may be amplified and the number of initial target molecules in the sample can be estimated by counting the number of counter sequences associated with the amplified target molecules.Type: ApplicationFiled: April 27, 2017Publication date: August 10, 2017Inventors: Robert Osborne, James Casbon, Andreas Claas, Gi Mikawa, Esther Musgrave-Brown
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Patent number: 9670529Abstract: Described herein is a method for adding a counter sequence to the individual polynucleotide molecules of an initial nucleic acid sample. After addition of the counter sequence, the sample may be amplified and the number of initial target molecules in the sample can be estimated by counting the number of counter sequences associated with the amplified target molecules.Type: GrantFiled: February 26, 2013Date of Patent: June 6, 2017Assignee: POPULATION GENETICS TECHNOLOGIES LTD.Inventors: Robert Osborne, James Casbon, Andreas Claas, Gi Mikawa, Esther Musgrave-Brown
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Publication number: 20160289753Abstract: A method of evaluating a sequence variation in a sample is provided. In some embodiments, the method may involve: amplifying a nucleic acid product from an initial sample; fragmenting an amount of the nucleic acid product to produce fragments; attaching an adaptor to each end of the fragments to produce adaptor-tagged fragments; sampling no more than 10% of the tagged fragments and amplifying them; sequencing at least some of the copies of the fragments to produce a plurality of sequence reads; grouping sequence reads for copies of fragments that have the same fragmentation breakpoints; deriving a consensus sequence for each of the read groups; and aligning the consensus sequences with a reference sequence.Type: ApplicationFiled: November 26, 2014Publication date: October 6, 2016Inventors: Robert OSBORNE, Esther MUSGRAVE-BROWN