Patents by Inventor Etsuko Miyamoto

Etsuko Miyamoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11052154
    Abstract: A Ras protein degradation inducing molecule that can induce degradation of Ras proteins, and a pharmaceutical composition that contains this Ras protein degradation inducing molecule are provided. The Ras protein degradation inducing molecule is a conjugate of a Ras protein affinity molecule which has affinity to Ras proteins, and a proteolysis-inducing tag which has affinity to protease and does not inhibit proteolysis of proteins by the protease.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: July 6, 2021
    Assignee: Tokyo University of Science Foundation
    Inventors: Etsuko Miyamoto, Masaaki Ozawa
  • Patent number: 11007269
    Abstract: A p53 degradation inducing molecule which can induce degradation of p53 proteins or p53 composites, and a pharmaceutical composition containing said p53 degradation inducing molecule are provided. This p53 degradation inducing molecule is a conjugate of a p53 affinity molecule which has affinity for p53 proteins or p53 composites, and a proteolysis induction tag which has affinity for protease and which does not inhibit proteolysis of proteins by protease.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: May 18, 2021
    Assignee: Tokyo University of Science Foundation
    Inventors: Etsuko Miyamoto, Masaaki Ozawa
  • Patent number: 10976306
    Abstract: Provided are: a protein degradation inducing tag which is a molecule that has affinity with proteases and does not inhibit degradation of a protein by proteases; a protein degradation inducing molecule that is a conjugate of at least one protein degradation inducing tag and at least one protein binding molecule that binds to a protein; and a usage of those.
    Type: Grant
    Filed: June 15, 2016
    Date of Patent: April 13, 2021
    Assignee: Tokyo University of Science Foundation
    Inventors: Etsuko Miyamoto, Masaaki Ozawa
  • Publication number: 20210088504
    Abstract: Provided are: a protein degradation inducing tag which is a molecule that has affinity with proteases and does not inhibit degradation of a protein by proteases; a protein degradation inducing molecule that is a conjugate of at least one protein degradation inducing tag and at least one protein binding molecule that binds to a protein; and a usage of those.
    Type: Application
    Filed: December 2, 2020
    Publication date: March 25, 2021
    Applicant: Tokyo University of Science Foundation
    Inventors: Etsuko MIYAMOTO, Masaaki OZAWA
  • Publication number: 20200000927
    Abstract: A Ras protein degradation inducing molecule that can induce degradation of Ras proteins, and a pharmaceutical composition that contains this Ras protein degradation inducing molecule are provided. The Ras protein degradation inducing molecule is a conjugate of a Ras protein affinity molecule which has affinity to Ras proteins, and a proteolysis-inducing tag which has affinity to protease and does not inhibit proteolysis of proteins by the protease.
    Type: Application
    Filed: November 13, 2017
    Publication date: January 2, 2020
    Applicant: Tokyo University of Science Foundation
    Inventors: Etsuko MIYAMOTO, Masaaki OZAWA
  • Publication number: 20190369111
    Abstract: A molecular kinetics evaluation method is provided which involves: a step for dosing a human or non-human animal with a proteolysis-inducing molecule, which is a conjugate of a proteolysis induction tag, i.e., a molecule which has affinity for protease and which does not inhibit proteolysis by protease, and a specific protein affinity molecule having affinity to a specific protein, and inducing proteolysis of the specific protein in vivo in the human or non-human animal; and a step for evaluating the molecular kinetics of the specific protein affinity molecules or the proteolysis-inducing molecules by detecting proteolysis of the specific proteins in a sample which is at least part of the human or non-human animal.
    Type: Application
    Filed: November 13, 2017
    Publication date: December 5, 2019
    Applicant: Tokyo University of Science Foundation
    Inventors: Etsuko MIYAMOTO, Masaaki OZAWA
  • Publication number: 20190314508
    Abstract: A p53 degradation inducing molecule which can induce degradation of p53 proteins or p53 composites, and a pharmaceutical composition containing said p53 degradation inducing molecule are provided. This p53 degradation inducing molecule is a conjugate of a p53 affinity molecule which has affinity for p53 proteins or p53 composites, and a proteolysis induction tag which has affinity for protease and which does not inhibit proteolysis of proteins by protease.
    Type: Application
    Filed: November 13, 2017
    Publication date: October 17, 2019
    Applicant: Tokyo University of Science Foundation
    Inventors: Etsuko MIYAMOTO, Masaaki OZAWA
  • Publication number: 20180164289
    Abstract: Provided are: a protein degradation inducing tag which is a molecule that has affinity with proteases and does not inhibit degradation of a protein by proteases; a protein degradation inducing molecule that is a conjugate of at least one protein degradation inducing tag and at least one protein binding molecule that binds to a protein; and a usage of those.
    Type: Application
    Filed: June 15, 2016
    Publication date: June 14, 2018
    Applicant: Tokyo University of science Foundation
    Inventors: Etsuko MIYAMOTO, Masaaki OZAWA
  • Patent number: 9513295
    Abstract: The present invention provides a method for detecting an interaction, which method can solve not only the problem of false negatives but also the problem of false positives.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: December 6, 2016
    Assignee: TOKYO UNIVERSITY OF SCIENCE FOUNDATION
    Inventors: Etsuko Miyamoto, Shigeo Fujimori
  • Patent number: 9481882
    Abstract: A method for analyzing biomolecular interactions, which method can be carried out without performing affinity selection using an immobilized bait, is provided. mRNA portions of assignment molecules that interacted with each other are linked together by annealing via a DNA linker for linking mRNA portions of assignment molecules together, the DNA linker comprising, at the 5?-end, an mRNA-complementary region complementary to a sequence at the 5?-end of each mRNA portion, and, at the 3?-end, a self-complementary region complementary between molecules of the DNA linker, the DNA linker being phosphorylated at the 5?-end.
    Type: Grant
    Filed: March 31, 2011
    Date of Patent: November 1, 2016
    Assignee: TOKYO UNIVERSITY OF SCIENCE FOUNDATION
    Inventors: Etsuko Miyamoto, Toru Tsuji, Shigeo Fujimori, Masamichi Ishizaka
  • Publication number: 20140243209
    Abstract: The present invention provides a method for detecting an interaction, which method can solve not only the problem of false negatives but also the problem of false positives.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 28, 2014
    Applicant: The University of Tokyo
    Inventors: Etsuko Miyamoto, Shigeo Fujimori
  • Publication number: 20130196331
    Abstract: A method for analyzing biomolecular interactions, which method can be carried out without performing affinity selection using an immobilized bait, is provided. mRNA portions of assignment molecules that interacted with each other are linked together by annealing via a DNA linker for linking mRNA portions of assignment molecules together, the DNA linker comprising, at the 5?-end, an mRNA-complementary region complementary to a sequence at the 5?-end of each mRNA portion, and, at the 3?-end, a self-complementary region complementary between molecules of the DNA linker, the DNA linker being phosphorylated at the 5?-end.
    Type: Application
    Filed: March 31, 2011
    Publication date: August 1, 2013
    Inventors: Etsuko Miyamoto, Toru Tsuji, Shigeo Fujimori, Masamichi Ishizaka
  • Patent number: 8278427
    Abstract: An assigning molecule obtained by ligating a genotype molecule to a phenotype molecule by transpeptidation, wherein the genotype molecule is constructed by bonding (a) a spacer molecule comprising a donor region which can be bonded to a 3?-terminal end of nucleic acid, a PEG region bonded to the donor region and a peptide acceptor region which is bonded to the PEG region, to (b) a 3? end of a nucleic acid comprising a 5? untranslated region comprising a transcription promoter and a translation enhancer; an ORF region which comprises a polyA sequence and which is bonded to the 3?-terminal side of the 5? untranslated region; and a 3?-terminal region that is bonded to the 3?-terminal side of the ORF region.
    Type: Grant
    Filed: June 13, 2003
    Date of Patent: October 2, 2012
    Assignee: KEIO University
    Inventors: Hiroshi Yanagawa, Etsuko Miyamoto, Hideaki Takashima
  • Patent number: 7838243
    Abstract: Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Jun as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to provide known and unknown proteins which are unknown to form a complex with the c-Jun protein, whereby proteins that interact with c-Jun, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods are provided.
    Type: Grant
    Filed: November 19, 2004
    Date of Patent: November 23, 2010
    Assignee: Keio University
    Inventors: Etsuko Miyamoto, Kenichi Horisawa, Hiroshi Yanagawa
  • Patent number: 7670787
    Abstract: Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
    Type: Grant
    Filed: October 29, 2007
    Date of Patent: March 2, 2010
    Assignee: Keio University
    Inventors: Etsuko Miyamoto, Masamichi Ishizaka, Hiroshi Yanagawa
  • Publication number: 20080177030
    Abstract: Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
    Type: Application
    Filed: October 29, 2007
    Publication date: July 24, 2008
    Inventors: Etsuko Miyamoto, Masamichi Ishizaka, Hiroshi Yanagawa
  • Publication number: 20070003534
    Abstract: Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Jun as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to provide known and unknown proteins which are unknown to form a complex with the c-Jun protein, whereby proteins that interact with c-Jun, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods are provided.
    Type: Application
    Filed: November 19, 2004
    Publication date: January 4, 2007
    Inventors: Etsuko Miyamoto, Kenichi Horisawa, Hiroshi Yanagawa
  • Patent number: 7150978
    Abstract: A translation template comprising an ORF region coding for a protein, a 5? untranslated region comprising a transcription promoter and a translation enhancer and locating on the 5? side of the ORF region, and a 3? end region comprising a poly-A sequence and locating on the 3? side of the ORF region, is expressed in a translation system in the presence of an agent for modifying a C-terminal of a protein, which comprises an acceptor portion having a group capable of binding to a protein through a transpeptidation reaction in a protein translation system and a modifying portion comprising a nonradioactive modifying substance linked to the acceptor portion via a nucleotide linker, to cause protein synthesis and the synthesized protein is purified. Thus, the yield of modified protein in a method of modifying C-terminal of protein is improved and detection of protein interaction based on various intermolecular interaction detection methods is realized at an improved level.
    Type: Grant
    Filed: June 6, 2003
    Date of Patent: December 19, 2006
    Assignee: Keio University
    Inventors: Hiroshi Yanagawa, Nobuhide Doi, Etsuko Miyamoto, Hideaki Takashima, Rieko Oyama
  • Publication number: 20060183885
    Abstract: Proteins that interact with c-Fos, nucleic acids encoding them and inhibitors utilizing them as well as methods for detecting an interaction and screening methods utilizing a protein that interacts with c-Fos are provided. Comprehensive analysis of transcription control factor complexes in a mouse brain cDNA library with c-Fos as a bait by using the cotranslation selection and screening of in vitro virus (IVV) and the C-terminal labeling method are conducted, thereby to analyze proteins unknown so far, proteins known so far, but unknown to form a complex with the c-Fos protein, and so forth.
    Type: Application
    Filed: November 19, 2003
    Publication date: August 17, 2006
    Inventors: Etsuko Miyamoto, Masamichi Ishizaka, Hiroshi Yanagawa
  • Patent number: 7041446
    Abstract: A protein having a labeling compound attached to its C-terminal, in which the compound comprises a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in a cell-free protein synthesis system or in a living cell is provided. Also, a method for producing the protein comprising the step of performing synthesis of a protein in a cell-free protein synthesis system or in a living cell in the presence of a labeling compound comprising a label portion comprising a label substance and an acceptor portion comprising a compound having an ability of binding to a C-terminal of a synthesized protein when protein synthesis is carried out in the cell-free protein synthesis system or in the living cell, the labeling compound being present at a concentration effective for the labeling compound to bind to the C-terminal of the synthesized protein is provided.
    Type: Grant
    Filed: February 28, 2001
    Date of Patent: May 9, 2006
    Assignee: Mitsubishi Chemical Corporation
    Inventors: Hiroshi Yanagawa, Naoto Nemoto, Etsuko Miyamoto