Patents by Inventor Eugene Tan
Eugene Tan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8425861Abstract: A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.Type: GrantFiled: April 4, 2008Date of Patent: April 23, 2013Assignee: Netbio, Inc.Inventors: Richard F. Selden, Eugene Tan, Heung Chuan Lam, Heidi Susanne Giese, Gregory John Kellogg, John A. Wright
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Publication number: 20130032483Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.Type: ApplicationFiled: August 8, 2012Publication date: February 7, 2013Applicant: NETBIO, INC.Inventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
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Publication number: 20120309637Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.Type: ApplicationFiled: May 11, 2012Publication date: December 6, 2012Applicant: NetBio, Inc.Inventors: James W. Schumm, Richard F. Selden, Eugene Tan
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Publication number: 20120267247Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.Type: ApplicationFiled: May 29, 2012Publication date: October 25, 2012Applicant: NetBio Inc.Inventors: Eugene Tan, Heung Chuan Lam, Gregory John Kellogg
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Patent number: 8206974Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.Type: GrantFiled: May 19, 2005Date of Patent: June 26, 2012Assignee: NetBio, Inc.Inventors: Eugene Tan, Heung Chuan Lam, Greg Kellogg
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Patent number: 8173417Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.Type: GrantFiled: March 2, 2009Date of Patent: May 8, 2012Assignee: NetBio Inc.Inventors: Eugene Tan, Heung Chuan Lam, Gregory John Kellogg
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Publication number: 20120055798Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: ApplicationFiled: September 8, 2011Publication date: March 8, 2012Applicant: NetBio, Inc.Inventors: Richard F. Selden, Eugene Tan
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Publication number: 20110312614Abstract: A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.Type: ApplicationFiled: March 3, 2011Publication date: December 22, 2011Applicant: NetBio, Inc.Inventors: Richard F. Selden, Eugene Tan, Heung Chuan Lam, Heidi Susanne Giese, Gregory John Kellogg, John A. Wright
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Publication number: 20110220502Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.Type: ApplicationFiled: March 9, 2011Publication date: September 15, 2011Applicant: NetBio, Inc.Inventors: Richard F. Selden, Eugene Tan
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Patent number: 8018593Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.Type: GrantFiled: April 4, 2008Date of Patent: September 13, 2011Assignee: NetBio, Inc.Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
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Publication number: 20110195495Abstract: The inventive self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, comprises at least one input, and: (i) a macrofluidic component, comprising a chamber for receiving said unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with said macrofluidic component via at least one microfluidic element, said microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) a drive mechanism on said instrument for driving said liquid purification reagent, through said microfluidic element and said nucleic acid purification matrix, wherein the only inputs to said apparatus are via said chamber and said drive mechanism.Type: ApplicationFiled: February 11, 2011Publication date: August 11, 2011Applicant: NETBIO, INC.Inventors: Richard F. Selden, Eugene Tan
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Publication number: 20110008785Abstract: Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.Type: ApplicationFiled: June 15, 2010Publication date: January 13, 2011Applicant: NetBio, Inc.Inventors: Eugene Tan, Richard F. Selden, Rosemary S. Turingan
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Publication number: 20100285578Abstract: The inventive self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, comprises at least one input, and: (i) a macrofluidic component, comprising a chamber for receiving said unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with said macrofluidic component via at least one microfluidic element, said microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) a drive mechanism on said instrument for driving said liquid purification reagent, through said microfluidic element and said nucleic acid purification matrix, wherein the only inputs to said apparatus are via said chamber and said drive mechanism.Type: ApplicationFiled: February 3, 2010Publication date: November 11, 2010Applicant: NETWORK BIOSYSTEMS, INC.Inventors: Richard F. Selden, Eugene Tan
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Publication number: 20090229983Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.Type: ApplicationFiled: March 2, 2009Publication date: September 17, 2009Applicant: Network Biosystems, Inc.Inventors: Eugene Tan, Heung Chuan Lam, Gregory John Kellogg
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Publication number: 20090059222Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.Type: ApplicationFiled: April 4, 2008Publication date: March 5, 2009Applicant: Network Biosystems, Inc.Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
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Publication number: 20090020427Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.Type: ApplicationFiled: April 4, 2008Publication date: January 22, 2009Applicant: Network Biosystems Inc.Inventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
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Publication number: 20090023603Abstract: A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.Type: ApplicationFiled: April 4, 2008Publication date: January 22, 2009Applicant: NETWORK BIOSYSTEMS, INC.Inventors: Richard F. Selden, Eugene Tan, Heung Chuan Lam, Heidi Susanne Giese, Gregory John Kellogg, John A. Wright
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Publication number: 20060260941Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.Type: ApplicationFiled: May 19, 2005Publication date: November 23, 2006Inventors: Eugene Tan, Heung Lam, Gregory Kellogg