Patents by Inventor Eugene Vladislavovich Kirillov

Eugene Vladislavovich Kirillov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Use of continuous/contiguous stacking hybridization as a diagnostic tool
    Patent number: 6440671
    Abstract: A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength.
    Type: Grant
    Filed: February 7, 2000
    Date of Patent: August 27, 2002
    Assignee: The University of Chicago
    Inventors: Andrei Darievich Mirzabekov, Eugene Vladislavovich Kirillov, Sergei Valeryevich Parinov, Victor Evgenievich Barski, Svetlana Alekseevna Dubiley
  • Use of continuous/contiguous stacking hybridization as a diagnostic tool
    Patent number: 6090549
    Abstract: A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength.
    Type: Grant
    Filed: May 13, 1997
    Date of Patent: July 18, 2000
    Assignee: University of Chicago
    Inventors: Andrei Darievich Mirzabekov, Eugene Vladislavovich Kirillov, Sergei Valeryevich Parinov, Victor Evgenievich Barski, Svetlana Alekseevna Dubiley
  • Use of continuous/contiguous stacking hybridization as a diagnostic tool
    Patent number: 5908745
    Abstract: A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength.
    Type: Grant
    Filed: January 16, 1996
    Date of Patent: June 1, 1999
    Assignee: University of Chicago
    Inventors: Andrei Darievich Mirzabekov, Gennadiy Moseyevich Yershov, Eugene Vladislavovich Kirillov, Sergei Valeryevich Parinov, Victor Evgenievich Barski, Yuri Petrovich Lysov
  • Rapid method to detect duplex formation in sequencing by hybridization methods
    Patent number: 5861247
    Abstract: A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.
    Type: Grant
    Filed: January 26, 1996
    Date of Patent: January 19, 1999
    Assignee: University of Chicago
    Inventors: Andrei Darievich Mirzabekov, Edward Nikolaevich Timofeev, Vladimer Leonidovich Florentiev, Eugene Vladislavovich Kirillov