Abstract: A holographic imaging system may include an optical source configured to output a source beam and a splitter configured to split the source beam into a reference beam and an object beam that may be incident on a target to form a scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, an imaging array configured to receive the interference beam and generate frames of raw holographic data based on measurements of the interference beam over time, and an image data processor. The image data processor may be configured to receive the frames of raw holographic data from the imaging array, remove data components within the frames that are associated with the particle motion having a motion frequency that is less than a movement frequency threshold to form conditioned raw holographic data, and generate an image based on the conditioned raw holographic data.

Abstract: A holographic imaging system may include an optical source configured to output a source beam, a splitter configured to split the source beam into a reference beam and an object beam that is incident on a target to form a scattered object beam, and a pre-filter comprising a telecentric lens and a spatial filter. The pre-filter may be configured to receive the scattered object beam and filter diffuse light from the scattered object beam to form a filtered scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam.

Abstract: A holographic imaging system may include an optical source configured to output a source beam and a splitter configured to split the source beam into a reference beam and an object beam that may be incident on a target to form a scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, an imaging array configured to receive the interference beam and generate frames of raw holographic data based on measurements of the interference beam over time, and an image data processor. The image data processor may be configured to receive the frames of raw holographic data from the imaging array, remove data components within the frames that are associated with the particle motion having a motion frequency that is less than a movement frequency threshold to form conditioned raw holographic data, and generate an image based on the conditioned raw holographic data.

Abstract: A holographic imaging system may include an optical source configured to output a source beam, a splitter configured to split the source beam into a reference beam and an object beam that is incident on a target to form a scattered object beam, and a pre-filter comprising a telecentric lens and a spatial filter. The pre-filter may be configured to receive the scattered object beam and filter diffuse light from the scattered object beam to form a filtered scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam.

Abstract: A test device applies a defined mechanical load to a soft biomaterial such as a cell culture and a microscope forms a volume image data set showing the strain field or displacement field occurring in the medium. The data set is processed to determine fundamental mechanical properties of the cell, its interaction with the surrounding medium, or its responses loading or deformation of its surrounding medium. The device may also be used to calibrate or determine fundamental mechanical properties of the medium. The device includes a linear actuator that bears against the specimen, and adapted for a volume imaging device such as a scanning laser confocal microscope that forms a volume image data set of the specimen. The specimen may be supported in a Petri dish and is preferably imaged from below by an inverted microscope. Preferably the actuator device attaches to or forms the specimen stage of microscope.