Abstract: The invention relates to Mycobacterium tuberculosis superoxide dismutase antibodies, methods of using them for detection of M. tuberculosis, methods of testing for an inhibitor of an M. tuberculosis superoxide dismutase, and methods of detecting tuberculosis infection.

Abstract: The invention relates to Mycobacterium tuberculosis superoxide dismutase antibodies, methods of using them for detection of M. tuberculosis, methods of testing for an inhibitor of an M. tuberculosis superoxide dismutase, and methods of detecting tuberculosis infection.

Abstract: The invention relates to a transgenic knockout yeast which has a disruption in the gene encoding for a yeast ADP-ribosylation factor-like protein.

Abstract: The invention relates to Mycobacterium tuberculosis superoxide dismutase antibodies, methods of using them for detection of M. tuberculosis, methods of testing for an inhibitor of an M. tuberculosis superoxide dismutase, and methods of detecting tuberculosis infection.

Abstract: The invention relates to a transgenic knockout yeast which has a disruption in the gene encoding for a yeast ADP-ribosylation factor-like protein.

Abstract: This invention is directed to the acetyl-CoA hydrolase enzyme of yeast and to a method for purifying acetyl-CoA hydrolase. The enzyme has a molecular weight of about 64,000 daltons and an enzyme activity of greater than 20 units wherein one unit of acetyl CoA hydrolase activity is defined as the amount of enzyme able to inhibit 1 unit of acetyltransferase activity and 1 unit of acetyltransferase activity is defined as the amount of enzyme needed to transfer 1 pmol of [.sup.3 H] acetyl group from [.sup.3 H] acetyl coenzyme A to ACTH (1-24) in the acetyltransferase standard enzyme assay under standard conditions. The invention is further directed to the glucose-repressible promoter which regulates the expression of the acetyl-CoA hydrolase gene in yeast, to vectors incorporating this promoter and to host cells transformed with such vectors.

Abstract: This invention is directed to N.sup..alpha. -acetyltransferase with a molecular weight of about 180,000 daltons, said N.sup..alpha. -acetyltransferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than 100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N.sup..alpha. -acetyltransferase.

Abstract: A methionine N.sup..alpha. -acetyltransferase enzyme has been identified in yeast. The existence of the enzyme defines a gene for methionine N.sup..alpha. -acetyltransferase which may be cloned and altered. Cells expressing the normal or variant forms of this enzyme are valuable tools for determining the amino acid sequence of uncharacterized proteins. Such cells are also valuable tools for expressing a recombinant protein lacking an acetyl group at its .alpha.-amino group.

Abstract: A mutation defining a gene for the N.sup..alpha. -acetyltransferase of yeast has been identified and cloned. Mutations in this gene have been constructed and used to produce cells which are substantially incapable of catalyzing N.sup..alpha. -acetylation of protein. Such cells are valuable tools for determining the amino acid sequence of uncharacterized proteins. Such cells are also valuable tools for expressing a recombinant protein lacking an acetyl group at its .alpha.-amino group.

Abstract: This invention is directed to N.sup..alpha. -acetyltransferase with a molecular weight of about 180,000 daltons, said N.sup..alpha. -aceytl-transferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than 100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N.sup..alpha. -acetyltransferase.

Abstract: This invention is directed to N.sup..alpha. -acetyltransferase with a molecular weight of about 180,000 daltons, said N.sup..alpha. -acetyltransferase being composed of two subunit peptides having molecular weights of about 95,000 each, having enzyme activity greater than 100 wherein one unit of activity is defined as 1 pmol of acetyl residues incorporated into adrenocorticotropic hormone (ACTH) (amino acids 1-24) under standard assay conditions. This invention is further directed to a method for purifying the N.sup..alpha. -acetyltransferase.