Abstract: Agrobacterium rhizogenes are described herein along with methods of transforming cells and methods of using Agrobacterium rhizogenes and/or transformed cells. Also described herein are plants, such as plants in the Rubus family, including a modified nucleic acid, optionally wherein the plants are transgene-free and include a modified nucleic acid.

Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant by means of Agrobacterium-mediated transformation. Preferred is the use of the Zea may lines deposited with American Type Culture Collection under the Patent Deposit Designation PTA-6170 and PTA-6171.

Abstract: The present invention provides a PoC system (push-to-talk over cellular system) and a method for distributing media data in PoC. The PoC system includes a plurality of clients, a controlling server and a first participating server. In the present invention, since a media receiving port is established in the first participating server which manages at least two clients at the same time, and when a media data is going to be distributed to the clients managed by the first participating server, the controlling server delivers the media data to the media receiving port only. In addition, a multicast addressing technology is further used in the first participating server, therefore, the path of delivering media data is changed and the efficiency of media data delivery during a communication is improved.

Abstract: The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducing enzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed with a plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed.

Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant by means of Agrobacterium-mediated transformation. Preferred is the use of the Zea may lines deposited with American Type Culture Collection under the Patent Deposit Designation PTA-6170 and PTA-6171.

Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant based on a D-alanine or D-serine selection. Preferably the transformation is mediated by Agrobacterium.

Abstract: The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.

Abstract: The present invention provides a PoC system (push-to-talk over cellular system) and a method for distributing media data in PoC. The PoC system includes a plurality of clients, a controlling server and a first participating server. In the present invention, since a media receiving port is established in the first participating server which manages at least two clients at the same time, and when a media data is going to be distributed to the clients managed by the first participating server, the controlling server delivers the media data to the media receiving port only. In addition, a multicast addressing technology is further used in the first participating server, therefore, the path of delivering media data is changed and the efficiency of media data delivery during a communication is improved.

Abstract: The present invention provides methods of identifying transformed plants using the restoration of male fertility as a selectable marker. Preferably, the fertility restorer genes are used to restore fertility in Brassica napus. The present invention also includes vectors comprising male sterility genes flanked by recombinase sites, vector comprising a fertility restorer gene and a nucleotide sequence of interest flanked by recombinase sites, and methods of using such vectors to produce transgenic plants, using restoration of male fertility as the selection for transformation events.

Abstract: The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.