Patents by Inventor Femke Hoeksema

Femke Hoeksema has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Intergenic elements for enhancing gene expression
    Patent number: 10563223
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: February 18, 2020
    Assignee: CellaGenics B.V.
    Inventors: Arie Pieter Otte, Michel Siep, John Antonius Verhees, Femke Hoeksema, Henricus Johannes Maria Van Blokland
  • NOVEL INTERGENIC ELEMENTS FOR ENHANCING GENE EXPRESSION
    Publication number: 20180030476
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Application
    Filed: September 29, 2017
    Publication date: February 1, 2018
    Applicant: CellaGenics B.V.
    Inventors: Arie Pieter OTTE, Michel SIEP, John Antonius VERHEES, Femke HOEKSEMA, Henricus Johannes Maria VAN BLOKLAND
  • Intergenic elements for enhancing gene expression
    Patent number: 9790517
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Grant
    Filed: June 15, 2011
    Date of Patent: October 17, 2017
    Assignee: CellaGenics B.V.
    Inventors: Arie Pieter Otte, Michel Siep, John Antonius Verhees, Femke Hoeksema, Henricus Johannes Maria Van Blokland
  • Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
    Patent number: 9650630
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences from the promoter region of a 60S ribosomal protein L32 gene (RPL32), for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: May 16, 2017
    Assignee: CellaGenics B.V.
    Inventors: Arie Pieter Otte, Femke Hoeksema, John Antonius Verhees, Henricus Johannes Maria Van Blokland
  • NUCLEIC ACID FRAGMENTS FROM A RIBOSOMAL PROTEIN PROMOTER FOR ENHANCING GENE EXPRESSION
    Publication number: 20130236956
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences from the promoter region of a 60S ribosomal protein L32 gene (RPL32), for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Application
    Filed: August 31, 2011
    Publication date: September 12, 2013
    Inventors: Arie Pieter Otte, Femke Hoeksema, John Antonious Verhees, Henricus Johannes Maria Van Blokland
  • NOVEL INTERGENIC ELEMENTS FOR ENHANCING GENE EXPRESSION
    Publication number: 20130157312
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Application
    Filed: June 15, 2011
    Publication date: June 20, 2013
    Inventors: Arie Pieter Otte, Michel Siep, John Antonius Verhess, Femke Hoeksema