Abstract: The present invention relates to a method for optimization and recovery of second-generation sugar diluted stream, comprising pretreatment, enzymatic hydrolysis and mainly washing the residual solid from the enzymatic hydrolysis, which allows the increase of sugar recovery. The use of this sugar diluted stream is crucial for process integration, may have different possibilities of use, for example, can be applied in the mechanical refining step, microorganisms propagation, enzyme production, fermentation, enzymatic hydrolysis, including combination of uses.

Abstract: The invention provides monoclonal antibodies that neutralize TN? activity. The monoclonal antibodies may be rabbit monoclonal antibodies or monoclonal antibodies having CDR regions derived from those rabbit monoclonal antibodies. In certain embodiments, the monoclonal antibodies may be humanized. Methods of using the subject antibodies to inhibit TNF? activity, methods of treatment using those antibodies and kits containing the same are also provided. The invention finds use in a variety of research and medical applications.

Abstract: The invention provides a method for humanizing a rabbit monoclonal antibody. In general, the method involves comparing an amino acid sequences of a parent rabbit antibody to the amino acid sequences of a similar human antibody, and altering the amino acid sequence of the parent rabbit antibody such its framework regions are more similar in sequence to the equivalent framework regions of the similar human antibody. In many embodiments, amino acids in the parent rabbit antibody that are not CDR contact residues, interchain contact residues, or buried residues, are not modified. The invention further provides nucleic acids encoding the subject antibodies, as well as vectors and host cells comprising the nucleic acids and methods for producing a subject antibody. The subject antibodies, nucleic acid compositions and kits find use in a variety of applications, including diagnostics and therapeutic treatment and research of conditions and diseases.