Abstract: An approach is described that combines distinct properties of a specialized porous nitrocellulose film (PNC) with quantum nanoparticles to create an improved assay and detection sensitivity, permitting the development of a camera-based imaging system for fluorescent detection of macromolecules in microarray format. The two properties of PNC that facilitate the approach are an extraordinarily high binding capacity and a newly observed internal scattering of light. Quantum nanoparticles complement these PNC properties by providing a higher level of emitted light than the fluorescent dyes in common microarray use. Overall, the approach allows for instrument cost savings, reduced imaging time, and the ability to remotely image.

Abstract: Methods and systems are provided for imaging a microarray of a microarray assembly. The microarray assembly may be configured as a protein microarray and may be used to visualize protein expression levels in sample for disease detection and/or diagnosis. In one example, the microarray assembly may comprise a laser pointed in a first direction, a camera positioned parallel to and vertically below the laser, a first dichroic mirror vertically aligned with the laser for reflecting light emitted from the laser, a second mirror vertically aligned with the camera and horizontally aligned with the first dichroic mirror, and a chip coated in a nitrocellulose film and including an array of wells containing one or more biomolecules.

Abstract: Methods and systems are provided for imaging a microarray of a microarray assembly. The microarray assembly may be configured as a protein microarray and may be used to visualize protein expression levels in sample for disease detection and/or diagnosis. In one example, the microarray assembly may comprise a laser pointed in a first direction, a camera positioned parallel to and vertically below the laser, a first dichroic mirror vertically aligned with the laser for reflecting light emitted from the laser, a second mirror vertically aligned with the camera and horizontally aligned with the first dichroic mirror, and a chip coated in a nitrocellulose film and including an array of wells containing one or more biomolecules.

Abstract: An approach is described that combines distinct properties of a specialized porous nitrocellulose film (PNC) with quantum nanoparticles to create an improved assay and detection sensitivity, permitting the development of a camera-based imaging system for fluorescent detection of macromolecules in microarray format. The two properties of PNC that facilitate the approach are an extraordinarily high binding capacity and a newly observed internal scattering of light. Quantum nanoparticles complement these PNC properties by providing a higher level of emitted light than the fluorescent dyes in common microarray use. Overall, the approach allows for instrument cost savings, reduced imaging time, and the ability to remotely image.