Abstract: A method for analyzing spatial and temporal information of samples using optical microscopy includes choosing a field of view in a sample; scanning the sample by applying an electrical signal having a first frequency to an electrically tunable liquid lens in the detection path, performing a sequential acquisition, at a first acquisition frequency higher than the first frequency of the electrical signal, of a stack of images placed on different in-focus planes; processing the stack of images to identify the position of one or more regions of interest; scanning the sample by applying the electrical signal having a second frequency to the tunable lens, performing a sequential acquisition, at a second acquisition frequency lower than the second frequency of the electrical signal, of a temporal series of images with extended depth of field; and calculating the mean intensity of each region of interest for each image of the temporal series.

Abstract: A method for the optoinjection of exogenous material in a recipient biological cell is disclosed and comprises: placing a biological cell on a planar surface of a substrate, transmitting a sub-ns pulsed laser beam through a variable convergence/divergence collimator; focusing the laser beam in a focal spot positioned along an axial direction substantially perpendicular to the substrate; moving the focal spot towards the cell along the axial direction by continuously varying the electric control signal from a first amplitude value a second amplitude value the second amplitude value of the control signal is selected such that the second axial position is positioned inside the cell.

Abstract: A method for analyzing spatial and temporal information of samples using optical microscopy includes choosing a field of view in a sample; scanning the sample by applying an electrical signal having a first frequency to an electrically tunable liquid lens in the detection path, performing a sequential acquisition, at a first acquisition frequency higher than the first frequency of the electrical signal, of a stack of images placed on different in-focus planes; processing the stack of images to identify the position of one or more regions of interest; scanning the sample by applying the electrical signal having a second frequency to the tunable lens, performing a sequential acquisition, at a second acquisition frequency lower than the second frequency of the electrical signal, of a temporal series of images with extended depth of field; and calculating the mean intensity of each region of interest for each image of the temporal series.

Abstract: A method for the optoinjection of exogenous material in a recipient biological cell is disclosed and comprises: placing a biological cell on a planar surface of a substrate, transmitting a sub-ns pulsed laser beam through a variable convergence/divergence collimator; focusing the laser beam in a focal spot positioned along an axial direction substantially perpendicular to the substrate; moving the focal spot towards the cell along the axial direction by continuously varying the electric control signal from a first amplitude value a second amplitude value the second amplitude value of the control signal is selected such that the second axial position is positioned inside the cell.

Abstract: An optical and atomic force microscopy measurement integrated system is described. The system has an atomic force microscope having a first probe configured to interact with a sample to be analysed, an optical tweezer, a second probe configured to be held in the focus of the optical tweezer, movement means for moving the two probes, measurement means for measuring the variations of position of the two probes and processing means configured to receive, as an input, the measurement signals of the two probes to generate an output signal representative of the sample.