Abstract: The invention relates to method for the stepwise removal of bacteria from a fermentation broth. According to said method, the bacteria in the fermentation broth are mixed with a retention promoter mixture in a first process step and the pretreated bacteria are removed by precoat filtration in a second process step. The inventive method uses a retention promoter mixture from bentonite and polycations such as PEI or polyDADMAC and is suitable for treating large quantities of bacteria. The filter cake obtained with bentonite/PEI has a substantially improved quality.

Abstract: The method of making the clear cell lysate containing plasmid DNA from a biomass obtained in a cell culture process includes filtering the biomass obtained in the cell culture process with a filter medium in the presence of diatomaceous earth acting as a filtering agent to form a filter cake containing the biomass and the filter medium, then thermally digesting the biomass in the filter cake to form a cell lysate containing the plasmid DNA and filtering the cell lysate to form a clear filtrate. The clear filtrate contains the plasmid DNA and has a clarity characterized by an OD600 of at maximum 0.05 U/cm.

Abstract: The method of separating reversibly thermally precipitable oligomeric N-substituted (meth)acrylamides and conjugates thereof from aqueous solution includes thermally precipitating the oligomeric N-substituted (meth)acrylamides and the conjugates thereof from an aqueous solution in the presence of at least one salt and then filtering in the presence of at least one filter aid. The filter aid has a wet density of 0.2–0.3 g/cc and comprises?99% cellulose with fiber lengths<75 ?m (99%) and=?m (65%). The salt can be a potassium, sodium, ammonium, calcium and/or magnesium chloride, acetate and/or sulfate salt and can be present in the aqueous solution in a concentration between 0.01 M and 3 M.

Abstract: The invention relates to a method for the integrated treatment of biomass from a cell culture process for producing clear cell lysate containing plasmid DNA. According to the invention, in a first step filtering occurs in a filter in the presence of a filtering agent. In a second step, the biomass contained in the filter cake which is obtained is thermally decomposed and the cell lysate is filtered in another step. The obtained cell lysate containing plasmid DNA is characterised by a clarity of OD600 of at a maximum of 0.05 E/cm and which is suitable for cloning, transformation, transfection, microinjection in cells, gene therapy, DNA vaccination and/or for polymerase chain reaction (PCR).

Abstract: The invention relates to a method for the separation of oligomeric N-substituted (meth)acrylamides and conjugates thereof (enzyme conjugates, affinity macroligands and AML complexes) which are reversibly thermally precipitating from aqueous solutions, whereby the oligomeric N-substituted (meth)acrylamides and conjugates thereof are precipitated thermally and filtered in the presence of salts and filtration adjuncts. The oligomeric N-substituted (meth)acrylamides and conjugates thereof are characterised by a separation rate of over 90% of the thermal precipitate and thus permit an efficient biocatalysis and affinity preparation based on oligomeric compounds which are reversibly thermally precipitating on a large scale.