Patents by Inventor Frank Laue

Frank Laue has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid amplification in the presence of modified randomers
    Patent number: 8592184
    Abstract: The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
    Type: Grant
    Filed: December 12, 2012
    Date of Patent: November 26, 2013
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Dieter Heindl, Waltraud Ankenbauer, Frank Laue
  • Nucleic Acid Amplification in the Presence of Modified Randomers
    Publication number: 20130109060
    Abstract: The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
    Type: Application
    Filed: December 12, 2012
    Publication date: May 2, 2013
    Inventors: Dieter Heindl, Waltraud Ankenbauer, Frank Laue
  • PCR hot start by magnesium sequestration
    Patent number: 8026058
    Abstract: The present invention is directed to a synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so-called hot start effect.
    Type: Grant
    Filed: August 27, 2008
    Date of Patent: September 27, 2011
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Waltraud Ankenbauer, Dieter Heindl, Frank Laue
  • Thermostable nucleic acid polymerase from
    Patent number: 8008054
    Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Grant
    Filed: June 3, 2010
    Date of Patent: August 30, 2011
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
  • Polyanion for improved nucleic acid amplification
    Patent number: 7910720
    Abstract: The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3?m?6, 30?n?60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: March 22, 2011
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Waltraud Ankenbauer, Dieter Heindl, Frank Laue, Eva Walter, Renate Kolb
  • Thermostable Nucleic Acid Polymerase From Thermococcus Gorgonarius
    Publication number: 20110020898
    Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Application
    Filed: June 3, 2010
    Publication date: January 27, 2011
    Applicant: Roche Diagnostics GmBH
    Inventors: Waltraud ANKENBAUER, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
  • PCR HOT START BY MAGNESIUM SEQUESTRATION
    Publication number: 20100285535
    Abstract: The present invention is directed to a synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so-called hot start effect.
    Type: Application
    Filed: August 27, 2008
    Publication date: November 11, 2010
    Inventors: Waltraud Ankenbauer, Dieter Heindl, Frank Laue
  • POLYANION FOR IMPROVED NUCLEIC ACID AMPLIFICATION
    Publication number: 20100255547
    Abstract: The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3?m?6, 30?n?60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an —OH group or a phosphate group, and further provided that the terminal Y can also be an —OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.
    Type: Application
    Filed: August 25, 2009
    Publication date: October 7, 2010
    Inventors: Waltraud Ankenbauer, Dieter Heindl, Frank Laue, Eva Walter, Renate Kolb
  • Thermostable nucleic acid polymerase from
    Patent number: 7759107
    Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Grant
    Filed: June 9, 2008
    Date of Patent: July 20, 2010
    Assignee: Roche Diagnostics GmbH
    Inventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
  • NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF MODIFIED RANDOMERS
    Publication number: 20090269766
    Abstract: The present invention is directed to a composition comprising a DNA Polymerase which is preferably thermostable, Deoxynucleotides, at least one primer oligonucleotide or a pair of amplification primers, and randomized 5-8 mer oligonucleotide, characterized in that said oligonucleotide comprises a modification with an organic hydrophobic moiety Such a composition is specifically useful for performing hot start PCR.
    Type: Application
    Filed: March 18, 2009
    Publication date: October 29, 2009
    Inventors: Dieter Heindl, Waltraud Ankenbauer, Frank Laue
  • Detection format for hot start real time polymerase chain reaction
    Publication number: 20090181401
    Abstract: The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3?-5? exonuclease having a temperature optimum above 37° C.
    Type: Application
    Filed: March 19, 2009
    Publication date: July 16, 2009
    Inventors: Dieter Heindl, Waltraud Ankenbauer, Frank Laue
  • THERMOSTABLE NUCLEIC ACID POLYMERASE FROM THERMOCOCCUS GORGONARIUS
    Publication number: 20090093043
    Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Application
    Filed: June 9, 2008
    Publication date: April 9, 2009
    Applicant: Roche Diagnostics GmBH
    Inventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
  • Thermostable nucleic acid polymerase from
    Patent number: 7425423
    Abstract: A purified thermostable enzyme is derived from the thermophilic archaebacterium Thermococcus gorgonarius. The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3?-5? proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Grant
    Filed: October 1, 1997
    Date of Patent: September 16, 2008
    Assignee: Roche Diagnostics GmbH
    Inventors: Waltraud Ankenbauer, Vitaly Svetlichny, Elizaveta Bonch-Osmolovskaya, Christine Ebenbichler, Bernhard Angerer, Gudrun Schmitz-Agheguian, Frank Laue
  • Thermostable enzyme promoting the fidelity of thermostable DNA polymerases—for improvement of nucleic acid synthesis and amplification in vitro
    Patent number: 7410782
    Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3?-5? exonuclease and cleaves to produce 5?-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Taq especially for nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Grant
    Filed: September 29, 2005
    Date of Patent: August 12, 2008
    Assignees: Roche Diagnostics GmbH, Roche Molecular Systems, Inc.
    Inventors: Waltraud Ankenbauer, Frank Laue, Harald Sobek, Michael Greif
  • Composition and method for hot start nucleic acid amplification
    Patent number: 7122355
    Abstract: The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3?-5? Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3? terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
    Type: Grant
    Filed: July 11, 2002
    Date of Patent: October 17, 2006
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Waltraud Ankenbauer, Dieter Heindl, Frank Laue, Andreas Huber
  • Thermostable enzyme promoting the fidelity of thermostable DNA polymerases-for improvement of nucleic acid synthesis and amplification in vitro
    Patent number: 7030220
    Abstract: A purified thermostable enzyme is derived from the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3?–5? exonuclease and cleaves to produce 5?-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Tag especially for nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Grant
    Filed: September 27, 2000
    Date of Patent: April 18, 2006
    Assignees: Roche Diagnostics GmbH, Roche Molecular Systems Inc.
    Inventors: Waltraud Ankenbauer, Frank Laue, Harald Sobek, Michael Greif
  • Thermostable enzyme promoting the fidelity of thermostable DNA polymerases-for improvement of nucleic acid synthesis and amplification in vitro
    Publication number: 20060078928
    Abstract: A purified thermostable enzyme is derived form the thermophilic archaebacterium Archaeoglobus fulgidus. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3?-5? exonuclease and cleaves to produce 5?-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like Taq especially for nucleic acid amplification by the polymerase chain reaction (PCR).
    Type: Application
    Filed: September 29, 2005
    Publication date: April 13, 2006
    Applicants: Roche Diagnostics GmbH, Roche Molecular Systems Inc.
    Inventors: Waltraud Ankenbauer, Frank Laue, Harald Sobek, Michael Greif
  • Detection format for hot start real time polymerase chain reaction
    Publication number: 20050037410
    Abstract: The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3?-5? exonuclease having a temperature optimum above 37° C.
    Type: Application
    Filed: July 30, 2004
    Publication date: February 17, 2005
    Inventors: Dieter Heindl, Waltraud Ankenbauer, Frank Laue
  • Composition and method for hot start nucleic acid amplification
    Publication number: 20030119150
    Abstract: The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.
    Type: Application
    Filed: July 11, 2002
    Publication date: June 26, 2003
    Inventors: Waltraud Ankenbauer, Dieter Heindl, Frank Laue, Andreas Huber
  • Thermostable DNA polymerase from carboxydothermus hydrogenoformans
    Patent number: 6468775
    Abstract: A DNA polymerase from a thermophilic eubacterium is provided. The DNA polymerase shows magnesium ion dependent reverse transcriptase activity and 3′-5′ exonuclease activity. The invention also includes recombinant plasmids and transformed host cells capable of producing the enzyme. The enzyme is classified into class EC 2.7.7.7., a DNA nucleotidyl transferase DNA-directed type.
    Type: Grant
    Filed: November 22, 1999
    Date of Patent: October 22, 2002
    Assignee: Roche Molecular Systems Inc.
    Inventors: Waltraud Ankenbauer, Ursula Markau, Vitaly Svetlichny, Gudrun Schmitz-Agheguian, Astrid Reiser, Bernhard Angerer, Christine Ebenbichler, Frank Laue, Elizaveta Bonch-Osmolovskaya