Abstract: A library of known intrinsic spectra is provided to identify at least one known material in a sample of interest. The library includes individual intrinsic spectra channels defined by the assignment of intrinsic spectra of at least one known material, and combinations thereof, so that the assigned intrinsic spectra of each intrinsic spectra channel is correlated to at least one known material. The at least one known material is identified in the sample of interest when intrinsic spectra obtained from the sample of interest is matched to an assigned intrinsic spectra of an intrinsic spectra channel of the library of known intrinsic spectra.

Abstract: A library of known intrinsic spectra is provided to identify at least one known material in a sample of interest. The library includes individual intrinsic spectra channels defined by the assignment of intrinsic spectra of at least one known material, and combinations thereof, so that the assigned intrinsic spectra of each intrinsic spectra channel is correlated to at least one known material. The at least one known material is identified in the sample of interest when intrinsic spectra obtained from the sample of interest is matched to an assigned intrinsic spectra of an intrinsic spectra channel of the library of known intrinsic spectra.

Abstract: The Intrinsic Hyper-Spectral Flow Cytometer (IHSFC) and its associated methodology, improves current flow cytometry by eliminating the need of associated hardware-based elements currently used for spectral data detection. The (IHSFC), rather than using narrow band lasers to excite or interrogate the analytes, the flow stream is excited by a wide wavelength range beam. The raw data generated by the (IHSFC) are as follows; forward light scatter, right angle light scatter, coherent spectral data and non-coherent spectral data. The intrinsic fluorescent spectral components are extracted from the coherent and non-coherent spectral data.