Patents by Inventor Frank Meulewaeter
Frank Meulewaeter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230374480Abstract: The present invention relates to the field of gene genome editing. In particular, it relates to the provision of a Cas12a enzyme having nickase activity, as well as the means and methods for the modification of a genomic locus of interest with a Cas12a enzyme having nickase activity and uses thereof.Type: ApplicationFiled: March 1, 2023Publication date: November 23, 2023Inventors: John VAN DER OOST, Ricardo Villegas WARREN, Maartje Janneke LUTEIJN, Raymond Hubert Josèphe STAALS, Wen Ying WU, David DE VLEESSCHAUWER, Katelijn D'HALLUIN, Frank MEULEWAETER
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Publication number: 20230203515Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.Type: ApplicationFiled: September 10, 2020Publication date: June 29, 2023Inventors: Frank Meulewaeter, Christophe Liseron-Monfils
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Publication number: 20230148071Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.Type: ApplicationFiled: November 30, 2020Publication date: May 11, 2023Inventors: Frank Meulewaeter, Christophe Liseron-Monfils
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Publication number: 20230075913Abstract: It was now found that the expression of a nucleotide sequence as described in the method of the invention in a plant cell results in much higher rates of indels compared to those seen in cells transformed with a control nucleic acid molecule. Thus, the invention is directed to codon-optimized Cas9 endonuclease-encoding polynucleotide. Accordingly, the present invention provides a method for modifying a target site in the genome of a plant cell, the method comprising providing one or more guide RNA and a Cas endonuclease to said plant cell, wherein said guide RNA and Cas endonuclease are capable of forming a complex that enables the Cas endonuclease to introduce a double strand break at said target site, and wherein the Cas9 endonuclease is expressed in the plant cell from a polynucleotide comprising an codon-optimized Cas9 endonuclease encoding nucleic acid molecule with a nucleotide sequence selected from the disclosed nucleotide sequences.Type: ApplicationFiled: November 30, 2020Publication date: March 9, 2023Inventors: David DE VLEESSCHAUWER, Frank MEULEWAETER, Timothy James GOLDS
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Publication number: 20220220495Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing promoters and the production of plants with enhanced expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.Type: ApplicationFiled: May 6, 2020Publication date: July 14, 2022Inventors: Frank Meulewaeter, Shirong Zhang, Christophe Liseron-Monfils
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Patent number: 11060103Abstract: The present invention relates to novel gene sequences encoding insecticidal proteins produced by Bacillus thuringiensis strains. Particularly, new chimeric genes encoding a CryIC, CryIB or CryID protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other such chimeric genes.Type: GrantFiled: December 29, 2016Date of Patent: July 13, 2021Assignee: BASF AGRICULTURAL SOLUTIONS SEED, US LLCInventors: Jeroen Van Rie, Frank Meulewaeter, Gerben Van Eldik
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Publication number: 20200277578Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.Type: ApplicationFiled: April 9, 2020Publication date: September 3, 2020Inventors: Frank MEULEWAETER, Ilse VAN DEN BRANDE, Stephen C. FRY, Kyle E. MOHLER, Lenka FRANKOVA, Tom J. SIMMONS, Claire HOLLAND, Andrew HUDSON
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Patent number: 10647965Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.Type: GrantFiled: August 31, 2018Date of Patent: May 12, 2020Assignees: BASF SE, THE UNIVERSITY COURT OF THE UNIVERSITY OF EDINBURGHInventors: Frank Meulewaeter, Ilse Van Den Brande, Stephen C. Fry, Kyle E. Mohler, Lenka Frankova, Tom J. Simmons, Claire Holland, Andrew Hudson
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Patent number: 10544423Abstract: The present application discloses a recombinant fiber-selective promoter region comprising a DNA molecule comprising a fiber specificity region of a cotton lipid transfer protein gene promoter, operably linked to a DNA molecule comprising a nucleotide sequence having at least 90% sequence identity to a nucleotide sequence of about 500 consecutive nucleotides of the 3? end of the FB8-like 2 promoter and use thereof to increase fiber-selective expression of products of interest in cotton fiber cells.Type: GrantFiled: October 15, 2015Date of Patent: January 28, 2020Assignee: BASF Agricultural Solutions Seed US LLCInventor: Frank Meulewaeter
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Publication number: 20190153458Abstract: Methods and means are provided to produce positively charged oligosaccharides in the plant cell wall by introducing into said plant cell a Nodulation C protein fused to a heterologous Golgi signal anchor sequence.Type: ApplicationFiled: November 2, 2018Publication date: May 23, 2019Inventors: Frank Meulewaeter, Bartel Vanholme
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Publication number: 20190085349Abstract: An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase from E. coli which is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore, methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.Type: ApplicationFiled: December 7, 2018Publication date: March 21, 2019Inventor: Frank Meulewaeter
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Patent number: 10202613Abstract: Methods and means are provided to produce positively charged oligosaccharides in the plant cell wall by introducing into said plant cell a Nodulation C protein fused to a heterologous Golgi signal anchor sequence.Type: GrantFiled: September 30, 2011Date of Patent: February 12, 2019Inventors: Frank Meulewaeter, Bartel Vanholme
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Patent number: 10174335Abstract: An isolated nucleic acid molecule is provided comprising a nucleotide sequence which encodes a glutamine:fructose-6-phosphate amidotransferase from E. coli which is particularly suitable for expression in cotton plant cells. The invention also relates to plant cells or plants, in particular to cotton plant cells or cotton plants which produce an increased amount of positively charged polysaccharides in their cell walls. Furthermore methods and means are provided to increase the content of positively charged polysaccharides in the cell walls of cotton cells, in particular in cotton fibers. Fibers obtained from such cotton plants have an altered chemical reactivity which can be used to attach reactive dyes or other textile finish reagents to these fibers.Type: GrantFiled: March 18, 2015Date of Patent: January 8, 2019Assignee: BASF SEInventor: Frank Meulewaeter
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Publication number: 20190002849Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.Type: ApplicationFiled: August 31, 2018Publication date: January 3, 2019Inventors: Frank Meulewaeter, Ilse Van Den Brande, Stephen C. Fry, Kyle E. Mohler, Lenka Frankova, Tom J. Simmons, Claire Holland, Andrew Hudson
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Patent number: 10093907Abstract: The present invention relates to a hetero-transglycosylase protein having cellulose:xyloglucan endotransglucosylase (CXE) activity in addition to mixed-linkage beta-glucan:xyloglucan endotransglucosylase (MXE) activity. The protein may comprise the amino acid sequence of any one of SEQ ID NOs: 2, 6 and 8 or a functional fragment thereof; or an amino acid sequence having at least 60% sequence identity to any one of SEQ ID NO: 2, 6 and 8, or to SEQ ID NO: 2 from amino acid 22 to 280, to SEQ ID NO: 6 from amino acid 26 to 283, or to SEQ ID NO: 8 from amino acid 29 to 287. The invention furthermore relates to an isolated nucleic acid encoding the protein described herein, a chimeric gene comprising, inter alia, the nucleic acid described herein, a vector comprising said chimeric gene, a host cell comprising said vector or said chimeric gene and an according transgenic plant. Further disclosed herein in are a method of producing a transgenic plant and a method of improving properties of cellulosic material.Type: GrantFiled: September 24, 2014Date of Patent: October 9, 2018Assignee: BASF SEInventors: Frank Meulewaeter, Ilse Van Den Brande, Stephen C. Fry, Kyle E. Mohler, Lenka Frankova, Tom J. Simmons, Claire Holland, Andrew Hudson
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Patent number: 9994621Abstract: The present invention relates to novel DNA sequences encoding insecticidal Cry1 C proteins derived from Bacillus thuringiensis, and their use in plants to control insect pests. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising a Cry1 C chimeric gene of the invention and at least one other chimeric gene, such as a chimeric gene encoding an insecticidal Cry1Ab protein, and methods of making or using such plant cells or plants.Type: GrantFiled: May 28, 2008Date of Patent: June 12, 2018Assignee: BAYER CROPSCIENCE N.V.Inventors: Veronique Gossele, Frank Meulewaeter, Bernadette Saey, Stefan Jansens
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Patent number: 9873882Abstract: Methods and means are provided to enhance the selective expression of transgenes under control of a fiber-selective promoter, in fiber cells, particularly cotton fiber cells by including target sites for naturally occurring microRNAs with a specific expression profile, particularly with a differential expression profile between cells leading to fibers and other cells of the fiber producing plant, into the transcribed region of genes of interest.Type: GrantFiled: May 13, 2014Date of Patent: January 23, 2018Assignees: Bayer CropScience NV, Texas Tech UniversityInventors: Frank Meulewaeter, Zhixin Xie, Gengxiang Jia, Arnab Ghosh, Forrest Sheng Bao
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Publication number: 20170247712Abstract: The present application discloses a recombinant fiber-selective promoter region comprising a DNA molecule comprising a fiber specificity region of a cotton lipid transfer protein gene promoter, operably linked to a DNA molecule comprising a nucleotide sequence having at least 90% sequence identity to a nucleotide sequence of about 500 consecutive nucleotides of the 3? end of the FB8-like 2 promoter and use thereof to increase fiber-selective expression of products of interest in cotton fiber cells.Type: ApplicationFiled: October 15, 2015Publication date: August 31, 2017Inventor: Frank Meulewaeter
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Patent number: 9670496Abstract: Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1× of at least one HPPD inhibitor, and methods for making such plants.Type: GrantFiled: March 7, 2014Date of Patent: June 6, 2017Assignees: Bayer CropScience N.V., Bayer CropScience AG, Bayer CropScience LPInventors: Kathleen D'Halluin, Jan Dittgen, Stefan Jansens, Frank Meulewaeter, Koen Weterings, Hal Moser, Thomas Wilde, Ruediger Hain, Udo Bickers, Linda Trolinder, Gary Henniger
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Publication number: 20170107534Abstract: The present invention relates to novel gene sequences encoding insecticidal proteins produced by Bacillus thuringiensis strains. Particularly, new chimeric genes encoding a CryIC, CryIB or CryID protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other such chimeric genes.Type: ApplicationFiled: December 29, 2016Publication date: April 20, 2017Inventors: Jeroen VAN RIE, Frank Meulewaeter, Gerben Van Eldik