Patents by Inventor Frank Thieme
Frank Thieme has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230256078Abstract: An immunogenic composition comprising at least one Norovirus antigen and at least one adjuvant which is at least one B subunit of an AB5 toxin such as cholera toxin subunit B (CTB) or the B subunit of heat-labile E. coli exotoxin LT (LTB).Type: ApplicationFiled: March 7, 2023Publication date: August 17, 2023Inventors: Vesna Blazevic, Maria Malm, Frank Thieme, Franziska Zarczowski, Andre Diessner, Victor Klimyuk
-
Patent number: 11633467Abstract: An immunogenic composition comprising at least one Norovirus antigen and at least one adjuvant which is at least one B subunit of an AB5 toxin such as cholera toxin subunit B (CTB) or the B subunit of heat-labile E. coli exotoxin LT (LTB).Type: GrantFiled: February 14, 2019Date of Patent: April 25, 2023Assignee: Icon Genetics GmbHInventors: Vesna Blazevic, Maria Malm, Frank Thieme, Franziska Jarczowski, Andre Diessner, Victor Klimyuk
-
Publication number: 20220152186Abstract: The invention provides a norovirus genogroup I VP1 capsid protein having an amino acid sequence wherein the amino acid sequence stretch in the P domain Ala-Ala-Leu-Leu/Val-His-Tyr is modified to Ala-Ala-Leu-LeuNal-Arg/Lys-Tyr.Type: ApplicationFiled: March 11, 2020Publication date: May 19, 2022Applicant: ICON GENETICS GMBHInventors: Franziska JARCZOWSKI, Andre DIESNER, Andre DIESNER, Frank THIEME
-
Publication number: 20210000941Abstract: An immunogenic composition comprising at least one Norovirus antigen and at least one adjuvant which is at least one B subunit of an AB5 toxin such as cholera toxin subunit B (CTB) or the B subunit of heat-labile E. coli exotoxin LT (LTB).Type: ApplicationFiled: February 14, 2019Publication date: January 7, 2021Inventors: Vesna Blazevic, Maria Malm, Frank Thieme, Franziska Jarczowski, Andre Diessner, Victor Klimyuk
-
Patent number: 10435704Abstract: A process of producing a recombinant glycoprotein in a plant, in cells of a plant, or in plant cells is provided. The process comprises expressing in said plant, in cells of said plant or in said plant cells a nucleic acid sequence encoding a polypeptide having an N-glycosylation site and co-expressing a nucleic acid sequence encoding a heterologous single-subunit oligosaccharyltransferase.Type: GrantFiled: June 3, 2014Date of Patent: October 8, 2019Assignee: Icon Genetics GmbHInventors: Franziska Jarczowski, Romy Kandzia, Frank Thieme, Victor Klimyuk, Yuri Gleba
-
Patent number: 10052370Abstract: Protein conjugate comprising a protein antigen for generating an immune response against the HER2/neu protein and an immunogenic carrier covalently bonded to said protein antigen, wherein said protein antigen (i) has a sequence segment of 300 or more contiguous amino acids of the amino acid sequence of SEQ ID NO: 1; or (ii) has a variant sequence segment of 300 or more amino acid residues, wherein the amino acid sequence of said variant sequence segment has at least 85% sequence identity to a sequence portion from SEQ ID: 1; or (iii) has a variant sequence segment of 300 or more amino acid residues and has from 1 to 10 substitutions, deletions or additions in said variant sequence segment compared to a sequence segment of 300 or more amino acid residues of the amino acid sequence of SEQ ID NO: 1 or 2.Type: GrantFiled: March 11, 2014Date of Patent: August 21, 2018Assignees: ICON GENETICS GMBH, UNIVERSITY OF SOUTHAMPTONInventors: Natalia Savelyeva, Franziska Jarczowski, Romy Kandzia, Anja Nickstadt, Frank Thieme, Victor Klimyuk, Yuri Gleba, Duc Bui-Minh, Freda K. Stevenson, Warayut Chotprakaikiat
-
Patent number: 10035994Abstract: A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialylation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.Type: GrantFiled: May 23, 2017Date of Patent: July 31, 2018Assignee: KENTUCKY BIOPROCESSING, INC.Inventors: Greg Pogue, Ernie Hiatt, Romy Kandzia, Stefan Werner, Frank Thieme, Tsafrir Mor, Steven Hume
-
Publication number: 20170313999Abstract: A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialylation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.Type: ApplicationFiled: May 23, 2017Publication date: November 2, 2017Applicant: Kentucky BioProcessing, Inc.Inventors: Greg Pogue, Ernie Hiatt, Romy Kandzia, Stefan Werner, Frank Thieme, Tsafrir Mor, Steven Hume
-
Patent number: 9688970Abstract: A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.Type: GrantFiled: July 31, 2014Date of Patent: June 27, 2017Assignee: Kentucky BioProcessing, Inc.Inventors: Greg Pogue, Ernie Hiatt, Romy Kandzia, Stefan Werner, Frank Thieme, Tsafrir Mor, Steven Hume
-
Publication number: 20160115498Abstract: A process of producing a recombinant glycoprotein in a plant, in cells of a plant, or in plant cells, comprising expressing in said plant, in cells of said plant or in said plant cells a nucleic acid sequence encoding a polypeptide, said polypeptide having an N-glycosylation site of consensus sequence Asn-X-Ser or Asn-X-Thr, X being any standard amino acid residue, wherein, if the Asn residue of said N-glycosylation site is assigned amino acid sequence position 0, (a) the amino acid residue at position +3 is selected from Thr, Ser, Gly, Leu, Ile, Val and Met; or (b) the amino acid residue at position ?1 is selected from Glu and Asp.Type: ApplicationFiled: June 3, 2014Publication date: April 28, 2016Inventors: Franziska Jarczowski, Romy Kandzia, Frank Thieme, Victor Klimyuk, Yuri Gleba
-
Publication number: 20160015796Abstract: Protein conjugate comprising a protein antigen for generating an immune response against the HER2/neu protein and an immunogenic carrier covalently bonded to said protein antigen, wherein said protein antigen (i) has a sequence segment of 300 or more contiguous amino acids of the amino acid sequence of SEQ ID NO: 1; or (ii) has a variant sequence segment of 300 or more amino acid residues, wherein the amino acid sequence of said variant sequence segment has at least 85% sequence identity to a sequence portion from SEQ ID: 1; or (iii) has a variant sequence segment of 300 or more amino acid residues and has from 1 to 10 substitutions, deletions or additions in said variant sequence segment compared to a sequence segment of 300 or more amino acid residues of the amino acid sequence of SEQ ID NO: 1 or 2.Type: ApplicationFiled: March 11, 2014Publication date: January 21, 2016Applicants: ICON GENETICS GMBH, UNIVERSITY OF SOUTHAMPTONInventors: Natalia Savelyeva, Franziska Jarczowski, Romy Kandzia, Anja Nickstadt, Frank Thieme, Victor Klimyuk, Yuri Gleba, Duc Bui-Minh, Freda K. Stevenson, Warayut Chotprakaikiat
-
Publication number: 20150184138Abstract: A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.Type: ApplicationFiled: July 31, 2014Publication date: July 2, 2015Inventors: Greg Pogue, Ernie Hiatt, Romy Kandzia, Stefan Werner, Frank Thieme, Tsafrir Mor
-
Patent number: 8883420Abstract: A process of inserting a nucleic acid sequence of interest into an acceptor nucleic acid is provided. The process comprises amplifying by PCR a DNA comprising in the following order a sequence segment U, a nucleic acid sequence segment of known nucleotide sequence K2, and a nucleic acid sequence segment of known sequence K3. The process further comprises treating the linear double-stranded DNA molecules from the PCR amplification with an exonuclease to obtain a single-stranded overhang at the first end of the DNA and a single-stranded overhang comprising nucleic acid segments K2 and K3 at the second end of the DNA. The process additionally comprises annealing the product of the exonuclease treatment to a linearized double-stranded acceptor nucleic acid which has been designed to complement the single-stranded overhangs of the product of the exonuclease treatment.Type: GrantFiled: October 8, 2009Date of Patent: November 11, 2014Assignee: Icon Genetics GmbHInventors: Sylvestre Marillonnet, Stefan Werner, Carola Engler, Romy Kandzia, Frank Thieme, Ernst Weber
-
Publication number: 20110263024Abstract: A process of inserting a nucleic acid sequence of interest into an acceptor nucleic acid, comprising the following steps: amplifying by PCR a DNA comprising in the following order a sequence segment U, a nucleic acid sequence segment of known nucleotide sequence K2 and a nucleic acid sequence segment of known sequence K3 using a forward primer defining a first end of the amplified DNA and a reverse primer defining a second end of the amplified DNA, said reverse primer terminating at its 3?-end in a nucleotide sequence of nucleic acid sequence segment K3; treating the linear double-stranded DNA molecules contained in the PCR product obtained in the previous step with an exonuclease to obtain a single-stranded overhang at the first end of the DNA and a single-stranded overhang comprising nucleic acid segments K2 and K3 at the second end of the DNA; and annealing the product of the previous step to a linearized double-stranded acceptor nucleic acid having at a first end thereof a single-stranded overhang compType: ApplicationFiled: October 8, 2009Publication date: October 27, 2011Applicant: ICON GENETICS GmbHInventors: Sylvestre Marillonnet, Stefan Werner, Carola Engler, Romy Kandzia, Frank Thieme, Ernst Weber
-
Patent number: 7969139Abstract: A Rogowski sensor is provided for measuring a current of an electrical conductor. The sensor has a Rogowski coil, which has an electrical winding resistor, and an integration device which is connected to the Rogowski coil and is intended to generate an output signal which is proportional to the electrical current to be measured by the Rogowski sensor. The integration device is formed by the winding resistor of the Rogowski coil and a capacitance which is connected to the two winding connections of the Rogowski coil, and for the output signal from the integration device to be formed by the voltage applied to the capacitance.Type: GrantFiled: December 12, 2007Date of Patent: June 28, 2011Assignee: Siemens AktiengesellschaftInventors: Jochen Ermisch, Uwe Hering, Frank Thieme, Ralf-Reiner Volkmar
-
Publication number: 20100013460Abstract: A Rogowski sensor is provided for measuring a current of an electrical conductor. The sensor has a Rogowski coil, which has an electrical winding resistor, and an integration device which is connected to the Rogowski coil and is intended to generate an output signal which is proportional to the electrical current to be measured by the Rogowski sensor. The integration device is formed by the winding resistor of the Rogowski coil and a capacitance which is connected to the two winding connections of the Rogowski coil, and for the output signal from the integration device to be formed by the voltage applied to the capacitance.Type: ApplicationFiled: December 12, 2007Publication date: January 21, 2010Applicant: SIEMENS AKTIENGESELLSCHAFTInventors: Jochen Ermisch, Uwe Hering, Frank Thieme, Ralf-Reiner Volkmar
-
Patent number: 6346805Abstract: For precise measurement of currents with high dynamic range, especially in gas-insulated voltage switchgear, a first signal of a first low-power measurement transformer and a second signal of a second nonmagnetic measurement transformer, operating, for example, according to the principle of a Rogowski coil or according to the Faraday effect, are compared. Depending on the degree of accuracy, one of the two signals is then selected and further processed. A deviation of the two signals from each other, especially their gradient, is referred to as comparison criterion. In a corresponding configuration, the two measurement transformers of different type are connected to a common processing component for signal processing.Type: GrantFiled: April 14, 1999Date of Patent: February 12, 2002Assignee: Siemens AktiengesellschaftInventors: Jochen Ermisch, Uwe Stephan, Frank Thieme, Peter Bauerschmidt