Patents by Inventor Frank Wouter
Frank Wouter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240102028Abstract: The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.Type: ApplicationFiled: November 30, 2023Publication date: March 28, 2024Inventors: Dennis De Lange, Marc Anton Bernhard Kolkman, Frank Wouter Koopman, Chris Leeflang
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Patent number: 11879127Abstract: The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.Type: GrantFiled: August 21, 2018Date of Patent: January 23, 2024Assignee: DANISCO US INC.Inventors: Dennis De Lange, Marc Anton Bernhard Kolkman, Frank Wouter Koopman, Chris Leeflang
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Patent number: 11866713Abstract: The present disclosure is generally related to compositions and methods for obtaining Bacillus licheniformis cells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modified Bacillus licheniformis cells/strains derived from parental B. licheniformis cells/strains comprising a variant rghR2 gene.Type: GrantFiled: February 22, 2018Date of Patent: January 9, 2024Assignee: DANISCO US INC.Inventors: Marc Anton Bernhard Kolkman, Steven D. Doig, Ryan L. Frisch, Hongxian He, Frank Wouter Koopman, Chris Leeflang
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Publication number: 20230193298Abstract: The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.Type: ApplicationFiled: August 21, 2018Publication date: June 22, 2023Inventors: Dennis De Lange, Marc Anton Bernhard Kolkman, Frank Wouter Koopman, Chris Leeflang
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Patent number: 11306319Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) ??-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell.Type: GrantFiled: October 27, 2016Date of Patent: April 19, 2022Assignee: Danisco US Inc.Inventors: Cristina Bongiorni, Marguerite A. Cervin, George England, Chao Zhu, Frank Wouter Koopman
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Publication number: 20200056193Abstract: The present disclosure is generally related to compositions and methods for obtaining Bacillus licheniformis cells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modified Bacillus licheniformis cells/strains derived from parental B. licheniformis cells/strains comprising a variant rghR2 gene.Type: ApplicationFiled: February 22, 2018Publication date: February 20, 2020Inventors: Marc Anton Bernhard Kolkman, Steven D. Doig, Ryan L. Frisch, Hongxian He, Frank Wouter, Chris Leeflang
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Publication number: 20190367932Abstract: The present disclosure is generally related to methods and compositions for obtaining genetic competence in non-competent or poorly competent Bacillus host cells and transformed Bacillus host cells derived therefrom. Thus, in certain, embodiments, a non-competent Bacillus host cell is made competent by the introduction of at least one copy of an exogenously introduced polynucleotide encoding a B. licheniformis ComS1 polypeptide. In other embodiments, a non-competent Bacillus host cell is made competent by the deletion or disruption of a native (endogenous) gene encoding a ComS2 polypeptide. In other embodiments, the Bacillus host cells made competent according to the compositions and methods of the instant disclosure are transformed with one or more polynucleotides encoding one or more a proteins of interest.Type: ApplicationFiled: January 17, 2018Publication date: December 5, 2019Inventors: Marc Anton Bernhard Kolkman, Frank Wouter Koopman, Chris Leeflang
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Publication number: 20180327757Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpoC gene encoding a variant RNA-polymerase (RNAP) ??-subunit polypeptide. In certain embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the variant ??-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell.Type: ApplicationFiled: October 27, 2016Publication date: November 15, 2018Inventors: Cristina Bongiorni, Marguerite A. CERVIN, George England, Chao Ahu, Frank Wouter Koopman
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Patent number: 9715692Abstract: An Internet/world-wide-web based keyword bid management system that allows advertisers on pay-per-click search engines (“PPCSEs”) to influence position (ranking) on said PPCSEs with the use of a rules based bidding engine. Keyword bid rules allow advertisers to influence desired rankings on PPCSEs based on time-of-day, competitor ranking, bid gaps, etc, or combination of said rules and allow advertisers to mimic human bidding behavior through an automated means. The system comprises of specialized software (“rules based bidding system”) that is hosted by an ASP (“application service provider”) so that all of the advertiser's interactions with said software can take place from any personal computer with Internet access and relatively recent Internet browser software without the requirement of having to download any additional software locally.Type: GrantFiled: April 12, 2004Date of Patent: July 25, 2017Assignee: Facebook, Inc.Inventors: David Gilbert Carlson, Frank Wouter Watervoort
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Publication number: 20160366894Abstract: A system for the continuous production and dispensing of a baking mass for the production of baked products such as, in particular, flat wafers, hollow wafers, wafer rolls, waffles or cakes includes a pre-mixing device for the mixing and/or storage of a first baking mass component and one or more pumps for the continuous conveyance of the first baking mass component from the pre-mixing device to a baking mass dispensing device. An after-mixing device is provided between the pre-mixing device and the baking mass dispensing device for the subsequent continuous addition of a powder such as, in particular, powder which tends to clump such as in particular flour, wheat flour, cereal flour, legume flour, powder having high water absorption, pre-gelatinized flour, pre-gelatinized starch and/or thickening agent. A method for the continuous production and dispensing of the baking mass is also provided.Type: ApplicationFiled: February 9, 2015Publication date: December 22, 2016Inventors: JOHANNES HAAS, JOSEF HAAS, STEFAN JIRASCHEK, FRANK WOUTERS
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Patent number: 9481900Abstract: The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).Type: GrantFiled: April 3, 2015Date of Patent: November 1, 2016Assignee: Purac Biochem B.V.Inventors: Harald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Frank Wouter Koopman, Adrianus Johannes Jozef Straathof, Johannes Hendrik De Winde
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Publication number: 20150203882Abstract: The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).Type: ApplicationFiled: April 3, 2015Publication date: July 23, 2015Applicant: Purac Biochem B.V.Inventors: Harald Johan RUIJSSENAARS, Nick Johannes Petrus Wierckx, Frank Wouter Koopman, Adrianus Johannes Jozef Straathof, Johannes Hendrik De Winde
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Patent number: 9045787Abstract: The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).Type: GrantFiled: September 2, 2010Date of Patent: June 2, 2015Assignee: Purac Biochem B.V.Inventors: Harald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Frank Wouter Koopman, Adrianus Johannes Jozef Straathof, Johannes Hendrik De Winde
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Publication number: 20120309918Abstract: The invention relates to a polypeptide having oxidoreductase activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4, or a variant polypeptide thereof having 45% or more sequence identity with the sequence of SEQ ID NO: 3. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) or production of 5-hydroxymethyl-2-furancarboxylic acid (HMF acid).Type: ApplicationFiled: September 2, 2010Publication date: December 6, 2012Applicant: DSM IP ASSETS B.V.Inventors: Harald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Frank Wouter Koopman, Adrianus Johannes Jozef Straathof, Johannes Hendrik De Winde
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Publication number: 20110086395Abstract: An isolated microorganism is provided which is Cupriavidus basilensis strain HMF14 Deposit number DSM 22875, and its use in a process for the in-situ detoxification of lignocelluloses hydrolysate.Type: ApplicationFiled: September 2, 2010Publication date: April 14, 2011Inventors: Frank Wouter KOOPMAN, Harald Johan Ruijssenaars, Nick Johannes Petrus Wierckx, Johannes Hendrik De Winde