Abstract: This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such methods.

Abstract: A raised pavement markers removal vehicle system and method for the removal of raised pavement markers from a roadway. As the vehicle is driven along a roadway, a grinder unit alongside the forward portion of the vehicle grinds away the raised pavement markers to be removed. A sensing unit senses the relative lateral positions of the grinder unit and the upcoming raised pavement markers to be removed. A controller receives position information from the sensing unit and sends appropriate commands to an alignment adjuster to match the lateral position of the grinder unit to the lateral position of the upcoming pavement markers to be removed. After removal by grinding, the debris from the removed raised pavement markers is swept from the roadway into a sweeper-hopper unit for transport to a suitable unloading place. A warning board mounted at the rear of the sweeper-hopper unit provides a visual warning to other users of the road.

Abstract: A raised pavement markers removal vehicle system and method for the removal of raised pavement markers from a roadway. As the vehicle is driven along a roadway, a grinder unit alongside the forward portion of the vehicle grinds away the raised pavement markers to be removed. A sensing unit senses the relative lateral positions of the grinder unit and the upcoming raised pavement markers to be removed. A controller receives position information from the sensing unit and sends appropriate commands to an alignment adjuster to match the lateral position of the grinder unit to the lateral position of the upcoming pavement markers to be removed. After removal by grinding, the debris from the removed raised pavement markers is swept from the roadway into a sweeper-hopper unit for transport to a suitable unloading place. A warning board mounted at the rear of the sweeper-hopper unit provides a visual warning to other users of the road.

Abstract: This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such methods.

Abstract: An automated stringline installation system includes a vehicle, a 3D control system, at least partly carried by the vehicle, for determining location information, and an adjustable arm assembly, mounted on the vehicle, that identifies the location of a relative point in a stringline installation using the location information determined by the 3D control system. The adjustable arm assembly includes an operational arm having a proximal end and a distal end, a string, dispensed from the operational arm, for use in a stringline installation, and a sensor used to determine information pertaining to the location of the distal end of the operational arm assembly relative to the proximal end of the operational arm.

Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.

Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.

Abstract: An automated stringline installation system includes a vehicle, a 3D control system, at least partly carried by the vehicle, for determining location information, and an adjustable arm assembly, mounted on the vehicle, that identifies the location of a relative point in a stringline installation using the location information determined by the 3D control system. The adjustable arm assembly includes an operational arm having a proximal end and a distal end, a string, dispensed from the operational arm, for use in a stringline installation, and a sensor used to determine information pertaining to the location of the distal end of the operational arm assembly relative to the proximal end of the operational arm.

Abstract: A method for distributing advertisements. The method comprises initiating a telephone call from a telephone and sending a request for an advertisement from the telephone, the request including a unique identifier associated with the advertisement and a telephone number associated with the telephone. The request is received at server for further processing. If the user is a registered user, then the ad is sent to the user's personal computer. If the user is not registered, then further processing enables the user to be registered. The ad is thereafter sent to the user.

Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.

Abstract: The present invention provides a replicatable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heteroloqus RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo or polynucleotide of interest. This invention also provides methods for determining the presence of concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.

Abstract: Ligation methods for manipulating nucleic acid stands and oligonucleotides utilizing hybridization arrays of immobilized oligonucleotides. The oligonucleotide arrays may be plain or sectioned, comprehensive or non-comprehensive. The immobilized oligonucleotides may in some cases be binary oligonucleotides having constant as well as variable segments. Some embodiments include amplification of ligated products.

Abstract: A condenser augmentation device for a steam-powered system is described. The system includes a steam boiler that has a condensate inlet and a steam outlet. The steam outlet is connected to an inlet of a steam-powered device. The steam-powered device has an exhaust outlet that is connected to a choke. The choke is connected to a condensing pipe that is routed to the condensate inlet of the boiler, forming a closed system. The choke contains an orifice that is of substantially smaller cross-section that either the exhaust outlet or the condensing pipe. By passing the exhaust steam through the orifice, the flow is reduced, resulting in rapid expansion, cooling and condensation of the exhaust steam, thus permitting a condensing pipe of a shorter length to be used. The choke may take several forms. The choke may be adapted to accept choke plates having various sized orifices.

Abstract: Nucleic acid hybridization probes having a first conformation when not interacting with a target and a second conformation when interacting with a target, and having the ability to bring a label pair into touching contact in one conformation but not the other, are labeled with a non-FRET pair of chromophores and generate a fluorescent or absorbance signal. Kits may include such probes, and assays, including multiplex assays, may utilize such probes.

Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.

Abstract: This invention provides a method for producing a mutant virus or viroid, or a mutant recombinant RNA molecule comprising a single stranded virus genome or a single stranded viroid genome, a circular virus genome or a circular viroid genome, or a segment of a virus genome or a segment of a viroid genome which comprises incubating a recombinant single-stranded RNA molecule comprising a recognition sequence for the binding of an RNA directed RNA polymerase, a sequence for the initiation of product strand synthesis by the polymerase and a heterologous sequence of interest derived from a different RNA molecule inserted at a specific site in the internal region of the recombinant molecule under appropriate selective conditions and for a sufficient period of time permitting the selection of a mutant population, the heterologous sequence of interest comprising the RNA of the single stranded virus genome or the single stranded viroid genome, the circular virus genome or the circular viroid genome or a segment of the v