Abstract: The present disclosure provides a method of preparing a high density culture of bacteria. The method provides a bioreactor comprising a fermentation tank, a perfusion system, a medium, and a cell separator; preparing an inoculum comprising a bacteria consortia with at least two bacteria species; inoculating the fermentation tank with the inoculum to form a fermentation culture; and growing the bacteria consortia until it has reached a target density of at least about 1012 CFU/mL.

Abstract: Disclosed herein are compositions including cells of defined sets of microbial species (for example, 3, 16, 18, 19, 21, or 22 microbial species). Also disclosed are methods of using the microbial compositions that include contacting soil, plants, plant parts, or seeds with the composition. The microbial compositions are also used in methods of degrading biological materials, such as chitin-containing biological materials.

Abstract: Methods for selecting a microbe for co-formulation with a carrier are provided. In some examples, the methods include identifying a microbe that comprises one or more dipicolinic acid (DPA) synthase genes, a microbe that expresses one or more DPA synthase proteins, and/or a microbe that produces DPA; and selecting the microbe for co-formulation with a carrier. The methods optionally include co-formulating the selected microbe with the carrier. In some examples, the methods include detecting one or more DPA synthase genes or one or more DpaA and/or DpaB proteins in a microbe. In other examples, the methods include detecting DPA in a microbe or medium containing a microbe, for example, utilizing a fluorescence assay. Microbial compositions including one or more microbes that comprise one or more DPA synthase genes, express one or more DPA synthase proteins and/or produce DPA are also provided.

Abstract: Compositions including a core particle including at least one fertilizer nutrient and a shell substantially covering the core particle, wherein the shell includes at least one polymer layer or wax layer and one or more species of microbes (such as a population of cells from at least one species of microbes) are provided. In some embodiments, at least one of the polymer layer and/or the wax layer includes the one or more species of microbes. In other examples, the one or more species of microbes are included between layers, for example, between two polymer layers, between two wax layers, or between a polymer layer and a wax layer. Methods of preparing the compositions are also provided. Methods of using the disclosed compositions that include contacting soil, plants, plant parts, or seeds with the composition are provided.

Abstract: A process for fermenting syngas and a fermentation medium provides high ethanol productivity while removing medium components that were previously thought to be essential. The process is effective for providing a specific STY of at least about 1 gram of ethanol/(L·day·gram cells) and for providing a selenium content in cell biomass exiting the fermentation of about 1 ppm or less.

Abstract: Compositions including a core particle including at least one fertilizer nutrient and a shell substantially covering the core particle, wherein the shell includes at least one polymer layer or wax layer and one or more species of microbes (such as a population of cells from at least one species of microbes) are provided. In some embodiments, at least one of the polymer layer and/or the wax layer includes the one or more species of microbes. In other examples, the one or more species of microbes are included between layers, for example, between two polymer layers, between two wax layers, or between a polymer layer and a wax layer. Methods of preparing the compositions are also provided. Methods of using the disclosed compositions that include contacting soil, plants, plant parts, or seeds with the composition are provided.

Abstract: Disclosed herein are compositions including cells of defined sets of microbial species (for example, 3, 16, 18, 19, 21, or 22 microbial species). Also disclosed are methods of using the microbial compositions that include contacting soil, plants, plant parts, or seeds with the composition. The microbial compositions are also used in methods of degrading biological materials, such as chitin-containing biological materials.

Abstract: A process for fermenting syngas and a fermentation medium provides high ethanol productivity while removing medium components that were previously thought to be essential. The process is effective for providing a specific STY of at least about 1 gram of ethanol/(L·day·gram cells) and for providing a selenium content in cell biomass exiting the fermentation of about 1 ppm or less.

Abstract: The invention provides an improved cell free amplification method capable of producing large quantities of therapeutic-quality nucleic acids and methods of using the synthesized nucleic acid in research, therapeutic and other applications—The methods combine several different state-of-the-art procedures and coordinate their applications to affordably synthesize nucleic acids for therapeutic purposes. It combines in vitro rolling circle amplification, high fidelity polymerases, high affinity primers, and streamlined template specifically designed for particular applications. For expression purposes, the templates contain an expression cassette including a eukaryotic promoter, the coding sequence for the gene of interest, and a eukaryotic termination sequence.