Abstract: A rotating cell vessel (4), designed to be simply constructed, and method of use have been developed. The vessel (4) is constructed of materials which are permeable to oxygen and carbon dioxide, and when rotated horizontally around central axis (10) suspends the contents, cells and particle substrates, in a liquid medium with a low turbulence, low shear fluid environment. The vessel walls (24) are made at least partially of gas permeable material. The vessel (4) is rotated to suspend the cells and substrate particles in a low turbulence liquid nutrient medium. Oxygen continuously diffuses through the permeable vessel material and through the cell culture liquid medium to provide the needed oxygen to the cells. Carbon dioxide diffuses through the cell culture medium and through the vessel wall (24), removing this waste product from the cell culture. A means for entrapping gas bubbles away from cell growth is also provided.

Abstract: A new rotating cell vessel and method of use have been developed. The vessel is constructed of materials which are permeable to oxygen and carbon dioxide, and when rotated horizontally suspend the contents, cells and particle substrates, in a liquid medium with a low turbulence, low shear fluid environment. The vessel walls are made at least partially of gas permeable material. The vessel is rotated in a horizontal plane to suspend the cells and substrate particles in a low turbulence liquid nutrient medium. Oxygen continuously diffuses through the permeable vessel material and through the cell culture liquid medium to provide the needed oxygen to the cells. Carbon dioxide diffuses through the cell culture medium and through the vessel wall, removing this waste product from the cell culture. The vessel is uniquely designed to be simply constructed.