Patents by Inventor Frederick R. Haselton
Frederick R. Haselton has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240084372Abstract: The present disclosure is directed to methods of detecting small fragments of known nucleic acid biomarkers.Type: ApplicationFiled: August 3, 2023Publication date: March 14, 2024Applicant: Vanderbilt UniversityInventors: Frederick R. HASELTON, David T. EVANS, Megan E. PASK, Emily C. KIGHT
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Publication number: 20230392205Abstract: The present disclosure is directed to the use of left-handed DNA (L-DNA) tracer to identify the source, track the distribution, and validate the integrity of products or resources that are highly regulated, valuable, or hazardous (e.g., pharmaceuticals, treated water, chemicals, designer products, and ammunitions). L-DNA tracers can encrypt unique identifying information, as well as more general information about the type of product, such as the manufacturing location, source, and date, directly into the nucleotide sequence. The L-DNA tracers can embed directly into the product so that it could neither be disassociated from the product nor be re-associated with another product. Because there are no technologies available to sequence L-DNA, the L-DNA tracers cannot be reverse engineered, copied, or falsified. The L-DNA tracers are only deciphered using a unique detection key.Type: ApplicationFiled: May 26, 2023Publication date: December 7, 2023Applicant: Vanderbilt UniversityInventors: Nicholas M. ADAMS, Frederick R. HASELTON
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Publication number: 20230251195Abstract: The number of unique testable conditions that can be detected in parallel from a sample in a PCR assay can be increased without need for larger sample volumes or additional expensive instruments. At least two fluorescent compounds, each designed to have a peak fluorescence excitation and/or emission within a common spectral channel, can be added to a sample in a tube, wherein each of the at least two fluorescent compounds is used to test for a unique testable condition. PCR assay can be performed on the contents of the tube. Then a spectral channel signal of each of the at least two fluorescent compounds can be determined based on fluorescence emission measurements and at least two properties of each of the at least two fluorescent compounds, wherein the spectral channel signals are analyzed to indicate a presence or absence of each of the unique testable conditions.Type: ApplicationFiled: February 3, 2023Publication date: August 10, 2023Inventors: Nicholas M. Adams, Frederick R. HASELTON, Zackary A. ZIMMERS
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Publication number: 20210371905Abstract: Disclosed herein is a COVID-19 surveillance method for detecting exhaled virions trapped within used face masks. This is a surveillance and warning system for identifying asymptomatic and pre-symptomatic individuals, particularly essential workers required to wear masks while at work. This can include healthcare workers, first responders, nursing home personnel, postal workers, or employees at meat packing and other production facilities. A piece of filter paper can be added to the inside of a standard face mask, which can be removed at the end of a shift. Mask inserts from a group of employees can be pooled and tested using standard RT-PCR for virions collected during normal exhalation over the time the mask is worn. As envisioned, if the group test is positive, additional follow-up or contact tracing could be initiated to identify the individual or individuals requiring treatment or quarantine.Type: ApplicationFiled: May 26, 2021Publication date: December 2, 2021Inventor: Frederick R. Haselton
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Patent number: 10968475Abstract: Systems, methods, and compositions for monitoring and analyzing nucleic acid hybridization state using L-DNA probes are described. The methods include adding L-DNA probes that can be fluorescently detected to a system including D-DNA. The L-DNA probes include primer, target, and antisense nucleotide sequences, and fluorescent dye compounds. The L-DNA probes are particularly useful for monitoring and analyzing various parameters during DNA amplification using the polymerase chain reaction.Type: GrantFiled: May 2, 2016Date of Patent: April 6, 2021Assignees: VANDERBILT UNIVERSITY, BIOVENTURES, INC.Inventors: Frederick R. Haselton, Nicholas M. Adams, Steven J. Simmons, Elliott P. Dawson
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Publication number: 20200122134Abstract: A device and method for performing low resource processing of biological or environmental samples is disclosed. The device uses high gradient magnetic separation to manipulate magnetic or paramagnetic beads for capture and isolation of analytes (proteins or nucleic acids) from solution. A disposable transfer pipette with tip containing a copper, aluminum or steel matrix is used to i) remove capture agent-coated magnetic beads bound to sample nucleic acids or proteins from the initial sample, ii) expose the analyte-bead complex to successive processing solutions and iii) separate the concentrated analytes from the beads in the final elution step.Type: ApplicationFiled: October 18, 2019Publication date: April 23, 2020Applicant: Vanderbilt UniversityInventors: Frederick R. HASELTON, Stephanie I. PEARLMAN
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Publication number: 20180171393Abstract: Systems, methods, and compositions for monitoring and analyzing nucleic acid hybridization state using L-DNA probes are described. The methods include adding L-DNA probes that can be fluorescently detected to a system including D-DNA. The L-DNA probes include primer, target, and antisense nucleotide sequences, and fluorescent dye compounds. The L-DNA probes are particularly useful for monitoring and analyzing various parameters during DNA amplification using the polymerase chain reaction.Type: ApplicationFiled: May 2, 2016Publication date: June 21, 2018Applicants: Vanderbilt University, BioVentures, Inc.Inventors: Frederick R. Haselton, Nicholas M. Adams, Steven J. Simmons, Elliott P. Dawson
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Patent number: 9575061Abstract: The present invention provides simple and inexpensive assays for the detection of virtually any analyte in any sample that is in liquid form or that can be solubilized. The assays utilize the fluid dynamics of drop evaporation whereby soluble materials, including analytes and particles binding thereto, are drawn to the edge of the drop and ultimately form a concentrated residual ring. The presence or absence of certain reagents can then be detected through a number of different approaches.Type: GrantFiled: October 20, 2010Date of Patent: February 21, 2017Assignee: VANDERBILT UNIVERSITYInventors: Frederick R. Haselton, Joshua Robert Trantum, David W. Wright
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Publication number: 20120276523Abstract: The present invention provides simple and inexpensive assays for the detection of virtually any analyte in any sample that is in liquid form or that can be solubilized. The assays utilize the fluid dynamics of drop evaporation whereby soluble materials, including analytes and particles binding thereto, are drawn to the edge of the drop and ultimately form a concentrated residual ring. The presence or absence of certain reagents can then be detected through a number of different approaches.Type: ApplicationFiled: October 20, 2010Publication date: November 1, 2012Applicant: VANDERBILT UNIVERSITYInventors: Frederick R. Haselton, Joshua Robert Trantum, David W. Wright
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Publication number: 20120003729Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: ApplicationFiled: July 1, 2011Publication date: January 5, 2012Applicant: VANDERBILT UNIVERSITYInventors: John P. Wikswo, Franz J. Baudenbacher, Ales Prokop, Eugene J. Leboeuf, Chang Y. Chung, David Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Publication number: 20110294202Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.Type: ApplicationFiled: May 26, 2011Publication date: December 1, 2011Applicant: VANDERBILT UNIVERSITYInventors: John P. Wikswo, Franz J. Baudenbacher, David Cliffel, Frederick R. Haselton, Eugene J. Leboeuf, Ales Prokop, Randall S. Reiserer, Mark A. Stremler
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Patent number: 8003378Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: GrantFiled: March 5, 2009Date of Patent: August 23, 2011Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Ales Prokop, Eugene LeBoeuf, Chang Y. Chung, David E. Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7977089Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.Type: GrantFiled: August 27, 2003Date of Patent: July 12, 2011Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, David Cliffel, Frederick R. Haselton, Eugene J. Leboeuf, Ales Prokop, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7790443Abstract: A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a chamber for receiving cells and a liquid medium. A port is formed in the second substrate between the bottom surface and the first surface of the second substrate. As formed, the port is in fluid communication with the chamber to allow a stream of substance to be introduced into the chamber. The stream of substance is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber.Type: GrantFiled: August 27, 2003Date of Patent: September 7, 2010Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Mark A. Stremler, Alissa Weaver
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Publication number: 20090215654Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: ApplicationFiled: March 5, 2009Publication date: August 27, 2009Applicant: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Ales Prokop, Eugene J. Leboeuf, Chang Y. Chung, David Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Patent number: 7534601Abstract: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.Type: GrantFiled: August 27, 2003Date of Patent: May 19, 2009Assignee: Vanderbilt UniversityInventors: John P. Wikswo, Franz J. Baudenbacher, Alex Prokop, Eugene J. Leboeuf, Chang Y. Chung, David Cliffel, Frederick R. Haselton, William H. Hofmeister, Charles P. Lin, Lisa J. McCawley, Randall S. Reiserer, Mark A. Stremler
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Publication number: 20040248125Abstract: A system 10 is provided for improved microarray biomolecular analysis. A microarray 36 is placed in a shallow chamber 20, and an induced motion of test fluid through the chamber is achieved by a sequential series of pulses directed to a plurality of source-sink pairs.Type: ApplicationFiled: July 26, 2004Publication date: December 9, 2004Inventors: Mark A Stremler, Timothy S. Fisher, Frederick R. Haselton, David K. Schaffer, Mark McQuain
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Methods for the selective regulation of DNA and RNA transcription and translation by photoactivation
Patent number: 6410327Abstract: The present invention provides an isolated nucleic acid covalently linked to a photolabile caging group which reversibly prevents expression of the nucleic acid. The present invention further provides a method of selectively expressing a nucleic acid in a cell, comprising: a) covalently linking the nucleic acid to a photolabile caging group which reversibly prevents expression of the nucleic acid; b) introducing the nucleic acid of step (a) into the cell; and c) exposing the cell of step (b) to light, whereby exposure to the light unlinks the nucleic acid and the caging group and the nucleic acid is selectively expressed in the cell.Type: GrantFiled: April 18, 2001Date of Patent: June 25, 2002Assignee: Vanderbilt University Office of Technology TransferInventors: Frederick R. Haselton, III, J. Steven Alexander -
Methods for the selective regulation of DNA and RNA transcription and translation by photoactivation
Publication number: 20010024830Abstract: The present invention provides an isolated nucleic acid covalently linked to a photolabile caging group which reversibly prevents expression of the nucleic acid. The present invention further provides a method of selectively expressing a nucleic acid in a cell, comprising: a) covalently linking the nucleic acid to a photolabile caging group which reversibly prevents expression of the nucleic acid; b) introducing the nucleic acid of step (a) into the cell; and c) exposing the cell of step (b) to light, whereby exposure to the light unlinks the nucleic acid and the caging group and the nucleic acid is selectively expressed in the cell.Type: ApplicationFiled: April 18, 2001Publication date: September 27, 2001Applicant: Vanderbilt UniversityInventors: Frederick R. Haselton, J. Steven Alexander -
Methods for the selective regulation of DNA and RNA transcription and translation by photoactivation
Patent number: 6242258Abstract: The present invention provides an isolated nucleic acid covalently linked to a photolabile caging group which reversibly prevents expression of the nucleic acid. The present invention further provides a method of selectively expressing a nucleic acid in a cell, comprising: a) covalently linking the nucleic acid to a photolabile caging group which reversibly prevents expression of the nucleic acid; b) introducing the nucleic acid of step (a) into the cell; and c) exposing the cell of step (b) to light, whereby exposure to the light unlinks the nucleic acid and the caging group and the nucleic acid is selectively expressed in the cell.Type: GrantFiled: January 5, 2000Date of Patent: June 5, 2001Assignee: Vanderbilt UniversityInventors: Frederick R. Haselton, III, J. Steven Alexander