Abstract: A microscope is modified to allow for precise imaging of samples using different filters at different focal planes under computer control. A filter accessory includes a filter disc having bandpass filters. The filter disc may be rotated to move a selected filter into the optical path between a light source and the microscope sample. Adjustment of the image plane of the microscope is controlled by the computer using feedback from a lens position sensor.

Abstract: A microscope is modified to allow for precise imaging of samples using different filters at different focal planes under computer control. A filter accessory includes a filter disc having bandpass filters. The filter disc may be rotated to move a selected filter into the optical path between a light source and the microscope sample. Adjustment of the image plane of the microscope is controlled by the computer using feedback from a lens position sensor.

Abstract: A series of spacial filters are used in a convolution process to determine location, size and orientation of bodies in an image. One series of spacial filters of different sizes is used to determine location and size. A second series is used to determine orientation and is a series of varying density, overlapping spacial filters. In particular, the series of spacial filters determines the spherical coordinants (r, .theta., .phi.) of bodies in an image of a muscle cell. The series of overlapping filters in one design have vector responses such that a resultant vector is defined. .phi. equals the angle of the resultant vector and .theta. is proportional to the length of the resultant vector. A series of one and two dimensional differential gaussian filters determine the cell boundaries.

Abstract: A microscope is modified to allow for precise imaging of samples using different filters at different focal planes under computer control. A filter accessory includes a filter disc having bandpass filters. The filter disc may be rotated to move a selected filter into the optical path between a light source and the microscope sample. Adjustment of the image plane of the microscope is controlled by the computer using feedback from a lens position sensor.

Abstract: An accessory is provided to convert a conventional microscope to a microspectrofluorimeter. The accessory includes a filter disc having bandpass filters separated by opaque segments. The filter disc is rapidly and continuously rotated to move the filter sequentially into the optical path between a wideband light source and a microscope sample. Photon counting of radiation from the sample is synchronized to the position of the filters during rotation by means of synchronization marks on the filter disc. The same accessory may be positioned between the sample and a detector to detect emitted light of different frequencies.