Abstract: This invention relates to a protein having phospholipase activity, which is characterised in that it has the mature sequence of Aspergillus lysophospholipase or a sequence derived therefrom and that it may be cleaved at at least one site, wherein, in the event of cleavage, the restriction fragments are optionally either linked by means of at least one bond cleavable under reducing conditions or at least one of the unlinked restriction fragments has phospholipase activity, and to a process for the production of this protein by fermenting a suitably transformed lysophospholipase-producing host organism in a suitable culture medium and isolating the protein having phospholipase activity from the cell-free culture filtrate, wherein fermentation is performed in the acidic to slightly alkaline range.

Abstract: This invention relates to a recombinant deoxyribonucleic acid (DNA) isolatable from Aspergillus, preferably Aspergillus foetidus, wherein it codes for a lysophospholipase (LPL) and has the nucleotide sequence for mature LPL stated in SEQ ID NO:1 or a nucleotide sequence derived therefrom, which hybridises under stringent conditions with the nucleotide sequence for mature LPL stated in SEQ ID NO:1. The invention also relates to vectors, to transformed host organisms and to processes for the production of LPL. The invention also provides enzyme products for the production of maltose syrup and products produced in this manner.

Abstract: An enzyme is purified by absorbing enzymes in an aqueous solution on a specific carrier material T-PbA to which is covalently bound phenyl butylamine ligand groups.