Abstract: The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features: (1) being positive for CD3, CD25, CD28 and T-cell antigen receptor ??; (2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8+) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4?CD8+) T-cell group; (3) exerting a cytotoxic activity against the cocultured stroma cells; and (4) exerting an immunosuppressive activity against activated T cells.

Abstract: A human T cell population which has both cytotoxic and immunosuppressive activities, is efficiently produced by first fractionating CD2-positive CD14-negative cells from mononuclear cells collected from a human umbilical cord blood, and then co-culturing them with stromal cells. The resulting blast cells, which have the desired activity, are proliferated by further culture.

Abstract: A polyurethane elastomeric filament-containing weft knit fabric is obtained by plating a bare yarn of highly fusible, alkali-resistant polyurethane elastomeric filament having at least 50% retention of tenacity following dry heat treatment under 100% extension at 150° C. for 45 seconds, a melting point of 180° C. or below, and at least 60% retention of tenacity following treatment in a 2 g/L aqueous sodium hydroxide solution under 100% extension at 100° C. for 60 minutes at every loop of a weft knit fabric having a 1×1 rib knit structure or a center yarn-containing reversible knit structure composed of at least one type of non-elastomeric yarn, then heat setting the plated structure so as to thermally fuse the highly fusible, alkali-resistant polyurethane elastomeric filaments to each other or to the non-elastomeric yarns at crossover points therebetween.

Abstract: The present invention has an object to provide a method for efficiently producing a human T cell population which has both cytotoxic and immunosuppressive activities, and solves the above object by providing a method for producing a human T cell population which has both cytotoxic and immunosuppressive activities, comprising the following steps (1) to (4): (1) fractionating mononuclear cells collected from a human umbilical cord blood into CD14-positive (CD14+) cells and CD14-negative (CD14?) cells, and then fractionating the CD14-negative (CD14?) cells into CD2-positive CD14-negative (CD2+CD14?) cells, and CD2-negative CD14-negative (CD2?CD14?) cells; (2) co-culturing the CD2-positive CD14-negative (CD2+CD14?) cells obtained in step (1) with stromal cells to generate blast cells; (3) adding the blast cells obtained in step (2) to the co-culture of the CD14-positive (CD14+) cells obtained in step (1) with stromal cells to allow the blast cells to proliferate; and (4) allowing the blast cells obtained in step

Abstract: The present invention has objects to provide a novel human T-cell population having both cytotoxic and immunosuppressive activities, and to a method for preparing the same. The above objects are attained by providing a human T-cell population which is obtainable by coculturing mononuclear cells, collected from human blood, with stroma cells and which has the following features: (1) being positive for CD3, CD25, CD28 and T-cell antigen receptor ??; (2) essentially consisting of three groups of a CD4 positive and CD8 positive (CD4+CD8?) T-cell group, a CD4 positive and CD8 dimly positive (CD4+CD8dim) T-cell group, and a CD4 negative and CD8 positive (CD4?CD8+) T-cell group; (3) exerting a cytotoxic activity against the cocultured stroma cells; and (4) exerting an immunosuppressive activity against activated T cells.

Abstract: A polyurethane elastomeric filament-containing weft knit fabric is obtained by plating a bare yarn of highly fusible, alkali-resistant polyurethane elastomeric filament having at least 50% retention of tenacity following dry heat treatment under 100% extension at 150° C. for 45 seconds, a melting point of 180° C. or below, and at least 60% retention of tenacity following treatment in a 2 g/L aqueous sodium hydroxide solution under 100% extension at 100° C. for 60 minutes at every loop of a weft knit fabric having a 1×1 rib. knit structure or a center yarn-containing reversible knit structure composed of at least one type of non-elastomeric yarn, then heat setting the plated structure so as to thermally fuse the highly fusible, alkali-resistant polyurethane elastomeric filaments to each other or to the non-elastomeric yarns at crossover points therebetween.