Abstract: The invention discloses a vitrification freezing treatment device for cells. The vitrification freezing treatment device for cells includes a straw device, a pre-freezing device, a freezing unit, a driving device, a control unit and a carrying table used for carrying cell carriers, wherein the straw device is connected with the driving device in a driven mode, the driving device is in signal connection with the control unit, the straw device comprises straws used for obtaining cells in the cell carriers, the control unit is used for controlling the driving device to drive the straw device to obtain cells and to transfer the cells to the freezing unit, and the pre-freezing device is used for pre-freezing the straws when the straw device transfers the obtained cells to the freezing unit.

Abstract: The invention discloses a vitrification freezing treatment device for cells. The vitrification freezing treatment device for cells includes a straw device, a pre-freezing device, a freezing unit, a driving device, a control unit and a carrying table used for carrying cell carriers, wherein the straw device is connected with the driving device in a driven mode, the driving device is in signal connection with the control unit, the straw device comprises straws used for obtaining cells in the cell carriers, the control unit is used for controlling the driving device to drive the straw device to obtain cells and to transfer the cells to the freezing unit, and the pre-freezing device is used for pre-freezing the straws when the straw device transfers the obtained cells to the freezing unit.

Abstract: The present invention relates to a vitrification method. In particular, the present invention relates to a method of producing at least one vitrified cell comprising loading a cell into a holding space in at least one conduit; providing at least one cryoprotectant to the holding space of the conduit in increasing concentrations, wherein the cryoprotectant is capable of equilibrating the cell; cooling the cell in the holding space of the conduit to produce a vitrified cell; and storing and maintaining the vitrified cell in the holding space of the conduit.

Abstract: The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and/or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.

Abstract: The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and/or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.

Abstract: The present invention relates to nuclear methods and embryos developed therefrom. In particular, the present invention relates to a method of nuclear comprising the step of transferring a somatic cell nuclei into a zona pellucida-free, enucleated oocyte.

Abstract: The present invention relates to nuclear transfer methods and embryos developed therefrom. In particular, the present invention relates to a method of nuclear transfer comprising the step of transferring a somatic cell or somatic cell nuclei into a zona pellucida-free, enucleated oocyte.

Abstract: Cells and tissues, in particular sensitive cells and tissues, such as oocytes, fertilized oocytes and preimplantation embryos, which require highly stable physical and chemical environment for in vitro development, are cultured in closed containers submerged or immersed in thermostatically controlled liquid baths, the containers being provided with an appropriate inner atmosphere containing, e.g. carbondioxide, oxygen and humidity in appropriate levels. The incubator containers may e.g. be gas and liquid impervious, flexible, sealable, preferably transparent bags which after sealing are submerged or immersed directly in the thermostatically controlled liquid bath. The liquid in the bath is preferably water. An incubator for submerse or immerse culture of cells and tissues in the above manner is also described. Also a transportable liquid incubator for culturing cells and tissues in the field is described.

Abstract: Cells and tissues, in particular sensitive cells and tissues, such as oocytes, fertilized oocytes and preimplantation embryos, which require highly stable physical and chemical environment for in vitro development, are cultured in closed containers submerged or immersed in thermostatically controlled liquid baths, the containers being provided with an appropriate inner atmosphere containing, e.g. carbondioxide, oxygen and humidity in appropriate levels. The incubator containers may e.g. be gas and liquid impervious, flexible, sealable, preferably transparent bags which after sealing are submerged or immersed directly in the thermostatically controlled liquid bath. The liquid in the bath is preferably water. An incubator for submerse or immerse culture of cells and tissues in the above manner is also described. Also a transportable liquid incubator for culturing cells and tissues in the field is described.