Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro. Specifically, the disclosure is directed to a prenyltransferase variant, a chimeric prenyltransferase comprising one or more portions of at least two different prenyltransferase proteins, and a fusion polypeptide comprising a CBG-type producing prenyltransferase and farnesyl pyrophosphate synthase.

Abstract: According to an example, a device comprises a sidewall and a base movable relative to the sidewall. The sidewall and base define an internal volume for receipt of build material in an additive manufacturing process. The example device further comprises a membrane. A first end of the membrane is attached to the base such that the membrane is movable with the base. The membrane at least partially extends along the sidewall to form a flexible barrier between the internal volume and the sidewall.

Abstract: Described herein are methods of preparing compounds derived from triglycerides or condensation polymers such as polyesters and/or polyamides. The methods may include subjecting triglyceride or condensation polymer containing matter to mechanical processing in the presence of a nucleophile.

Abstract: Embodiments described herein relate generally to plant extract compositions and methods to isolate fatty acid esters derived from crosslinked polyesters. Particular embodiments are directed to methods of preparing compositions of fatty acid esters by treating crosslinked polyesters or other crosslinked networks with an acid and an alcohol.

Abstract: Described herein are methods of preparing compounds derived from triglycerides or condensation polymers such as polyesters and/or polyamides. The methods may include subjecting triglyceride or condensation polymer containing matter to mechanical processing in the presence of a nucleophile.

Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro. In particular, the disclosure relates to variants of olivetolic acid cyclase.

Abstract: A garment made includes at least a waist portion, a first leg portion, and a second leg portion. The waist portion further includes a pocket on the front of the garment, where the pocket is defined by at least the waist portion and a backing panel. The backing panel may be constructed from a resilient material and is reconfigurable between an empty configuration and an expanded configuration. When in the empty configuration, the backing panel may include a plurality of folded panels and may be aligned with the waist portion. When in the expanded configuration, the backing panel may be stretched to extend beyond the waist portion. The backing panel is reconfigured from the empty configuration and the expanded configuration when an object is disposed within the pocket. The pocket is sized and shaped to receive small personal items, such as smartphones, keys, money, credit cards, identification, etc.

Abstract: Described herein are methods of preparing cutin-derived monomers, oligomers, or combinations thereof from cutin-containing plant matter. The methods can include heating the cutin-derived plant matter in a solvent at elevated temperature and pressure. In some preferred embodiments, the methods can be carried out without the use of additional acidic or basic species.

Abstract: The present disclosure relates to composite materials comprising a reinforcement material and a cationic polyelectrolyte, such as a porous reinforcement material impregnated with the cationic poly electrolyte. The present disclosure further relates to membrane electrode assemblies comprising the composites of the disclosure, and electrochemical devices comprising the disclosed membrane electrode assemblies.

Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

Abstract: A phase correction apparatus includes an inductor joined to a series capacitor to produce phase-corrected output having a load voltage and a load current. A phase detector is joined to the series capacitor and has a phase angle output proportional to a phase angle between the voltage and current. Logic circuitry or a processor is joined to receive the phase angle output and provide switch control value outputs to switches connected to the logic circuitry. A bank of parallel capacitors are joined to the switches. The switch control value outputs activate joined parallel capacitors to adjust reactance provided to the phase-corrected output to produce an adjusted output. In a further embodiment parallel capacitors are iterated to produce the adjusted output.

Abstract: Aspects of the disclosure relate to biosynthesis of histidine in host cells. For example, host cells may comprise: a promoter; a ribosome binding site (RBS); and a nucleic acid comprising: hisG; hisD; hisC hisB; hisH; hisA; hisF; and/or hisI. Host cells may further comprise a nucleic acid encoding a ribose phosphate pyrophosphokinase (RPPK), optionally comprising one or more amino acid substitutions relative to the sequence of wildtype E. coli RPPK. Host cells of the disclosure may comprise a nucleic acid encoding a 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase (MTHFDC) enzyme. Further aspects of the disclosure relate to production of purine pathway metabolites and/or plasmid DNA in host cells.

Abstract: The disclosure relates to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro comprising the use of disclosed prenyltransferase variants.

Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

Abstract: Embodiments described herein relate generally to plant extract compositions and methods to isolate fatty acid esters derived from crosslinked polyesters. Particular embodiments are directed to methods of preparing compositions of fatty acid esters by treating crosslinked polyesters or other crosslinked networks with an acid and an alcohol.

Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

Abstract: Described herein are methods of preparing compounds derived from triglycerides or condensation polymers such as polyesters and/or polyamides. The methods may include subjecting triglyceride or condensation polymer containing matter to mechanical processing in the presence of a nucleophile.

Abstract: Embodiments described herein relate generally to plant extract compositions and methods to isolate fatty acid esters derived from crosslinked polyesters. Particular embodiments are directed to methods of preparing compositions of fatty acid esters by treating crosslinked polyesters or other crosslinked networks with an acid and an alcohol.

Abstract: Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.