Abstract: There is provided a substrate for lysing cells and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.

Abstract: The present invention relates to methods for isolating and storing, nucleic acid from a sample containing nucleic acid, such as a cell sample or cell lysate. The nucleic acid is isolated on a solid phase medium, which is then dried, and which can be stored efficiently, such as at room temperature, in columns, tubes, and microwell plates having a wide variety of filters and other solid phase media, for extended periods of time, including days, weeks, and months. The invention provides methods for isolating and storing nucleic acid from a sample by applying the sample to a solid phase medium, retaining the cells, lysing the cellular retentate, drying the medium and retaining the nucleic acid, storing the nucleic acid for extended periods of time at room temperature and humidity, and optionally eluting the nucleic acid.

Abstract: There is provided a substrate for lysing calls and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.

Abstract: There is provided a substrate for lysing cells and purifying nucleic acid having a matrix and a coating and an integrity maintainer for maintaining the purified nucleic acid. Also provided is a method of purifying nucleic acid by applying a nucleic acid sample to a substrate having an anionic detergent affixed to a matrix, the substrate physically capturing the nucleic acid, bonding the nucleic acid to a substrate and generating a signal when the nucleic acid bonds to the substrate indicating the presence of the nucleic acid. A kit for purifying nucleic acid containing a coated matrix and an integrity maintenance provider for preserving the matrix and purifying nucleic acid is also provided.