Abstract: A PLL receives an indicator indicating that it is to operate at a different operating frequency than a current operating frequency. A control word is selected from a set of linear control words based upon the different operating frequency. A capacitance of a variable capacitor of a voltage-controlled oscillator is adjusted based upon the control word. The variable capacitor is monotonic and non-linear relative to the set of linear control words.

Abstract: A PLL receives an indicator indicating that it is to operate at a different operating frequency than a current operating frequency. A control word is selected from a set of linear control words based upon the different operating frequency. A capacitance of a variable capacitor of a voltage-controlled oscillator is adjusted based upon the control word. The variable capacitor is monotonic and non-linear relative to the set of linear control words.

Abstract: An overtone crystal oscillator automatic calibration system including an overtone crystal oscillator with multiple programmable resistors and multiple amplifiers with supply voltage inputs and a calibration system. The calibration system adjusts the programmable resistors and the supply voltage inputs and detects oscillation of the overtone crystal oscillator. The calibration system adjusts the programmable resistors and the supply voltage input for each of multiple sequential steps to adjust the frequency bandwidth, such as from a higher bandwidth and lower gain to a lower bandwidth at higher gain. For example, each resistance level is tested for each of multiple supply voltage levels. The range of resistances and voltages is designed to ensure oscillation at a selected overtone frequency while avoiding oscillation at a fundamental frequency of the oscillator crystal. Oscillation may be detected by a counter which counts to a predetermined count value indicating successful oscillation.

Abstract: A wide linear range peak detector including first and second peak detectors and a compensation circuit. The first peak detector receives an input signal and has an output providing a first peak signal approximation which approximates a peak level of the input signal. The first peak signal approximation includes a non-linear portion which is a function of the peak level of the input signal. The second peak detector also receives the input signal and has an output providing a second peak signal approximation. The compensation circuit uses the second peak signal approximation to provide a compensation signal which compensates the non-linear portion of the first peak signal approximation. In particular, the second peak signal is used to generate the compensation signal to approximate and cancel the non-linear portion.

Abstract: An overtone crystal oscillator automatic calibration system including an overtone crystal oscillator with multiple programmable resistors and multiple amplifiers with supply voltage inputs and a calibration system. The calibration system adjusts the programmable resistors and the supply voltage inputs and detects oscillation of the overtone crystal oscillator. The calibration system adjusts the programmable resistors and the supply voltage input for each of multiple sequential steps to adjust the frequency bandwidth, such as from a higher bandwidth and lower gain to a lower bandwidth at higher gain. For example, each resistance level is tested for each of multiple supply voltage levels. The range of resistances and voltages is designed to ensure oscillation at a selected overtone frequency while avoiding oscillation at a fundamental frequency of the oscillator crystal. Oscillation may be detected by a counter which counts to a predetermined count value indicating successful oscillation.

Abstract: The use of recombinant adeno-associated virus (AAV) virions for delivery of DNA molecules to muscle cells and tissue is disclosed. The invention allows for the direct, in vivo injection of recombinant AAV virions into muscle tissue, e.g., by intramuscular injection, as well as for the in vitro transduction of muscle cells which can subsequently be introduced into a subject for treatment. The invention provides for sustained, high-level expression of the delivered gene and for in vivo secretion of the therapeutic protein from transduced muscle cells such that systemic delivery is achieved.

Abstract: This invention relates to novel adenoviruses useful in the production of high titers of recombinant adeno-associated virus (rAAV) comprising a foreign DNA inert and methods of making these adenoviruses. The adenovirus comprises the AAV rep gene in which the p5 promoter of rep is replaced by a minimal promoter or by no promoter. The invention also provides methods of producing high levels of rAAV as a substantially homogeneous preparation and compositions of rAAV.

Abstract: A wide linear range peak detector including first and second peak detectors and a compensation circuit. The first peak detector receives an input signal and has an output providing a first peak signal approximation which approximates a peak level of the input signal. The first peak signal approximation includes a non-linear portion which is a function of the peak level of the input signal. The second peak detector also receives the input signal and has an output providing a second peak signal approximation. The compensation circuit uses the second peak signal approximation to provide a compensation signal which compensates the non-linear portion of the first peak signal approximation. In particular, the second peak signal is used to generate the compensation signal to approximate and cancel the non-linear portion.

Abstract: This invention relates to novel adenoviruses useful in the production of high titers of recombinant adeno-associated virus (rAAV) comprising a foreign DNA insert and methods of making these adenoviruses. The adenovirus comprises the AAV rep gene in which the p5 promoter is replaced by a minimal promoter or by no promoter. The invention also provides methods of producing high levels of rAAV as a substantially homogenous preparation and composition of rAAV.

Abstract: The present invention relates generally to immunization methods using recombinant viral vectors. In particular, the invention relates to methods and compositions for immunizing a subject with a nucleic acid molecule encoding an antigen of interest, wherein the nucleic acid molecule is delivered to the subject via a recombinant AAV virion.

Abstract: The use of recombinant adeno-associated virus (AAV) virions for delivery of DNA molecules to muscle cells and tissue in the treatment of anemia is disclosed, The invention allows for the direct, in vivo injection of recombinant AAV virions into muscle tissue, e.g., by intramuscular injection, as well as for the in vitro transduction of muscle cells which can subsequently be introduced into a subject for treatment. The invention provides for sustained, high-level expression of a delivered nucleotide sequence encoding erythropoietin, and for in vivo secretion thereof from transduced muscle cells such that systemic delivery is achieved.

Abstract: The use of recombinant adeno-associated virus (AAV) virions for delivery of DNA molecules to muscle cells and tissue is disclosed. The invention allows for the direct, in vivo injection of recombinant AAV virions into muscle tissue, e.g., by intramuscular injection, as well as for the in vitro transduction of muscle cells which can subsequently be introduced into a subject for treatment. The invention provides for sustained, high-level expression of the delivered gene and for in vivo secretion of the therapeutic protein from transduced muscle cells such that systemic delivery is achieved.

Abstract: The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

Abstract: The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

Abstract: The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

Abstract: The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

Abstract: The present invention provides methods and compositions for producing high titer preparations of recombinant AAV ("rAAV") virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small amounts of the long forms of Rep protein, and rAAV virions produced by such methods.

Abstract: A single-chip transceiver integrated circuit (100) has multiple on-chip circuits that implement receiver functions, transmitter functions, and audio processing functions. The IC (100) has interfaces (220, 240, 252, 270, 260, 245, 288, 290) which are situated among the on-chip circuits, and which couple one on-chip circuit to another. At least some of these on-chip interfaces (220, 240, 245, 252, 270) are configurable to couple an off-chip processing circuit to substitute for a corresponding on-chip circuit. In the preferred embodiment, the single-chip transceiver IC (100) supports radio configurations having off-chip versions of corresponding on-chip circuits for performing receiver front-end functions, synthesizer functions, reference oscillator functions, and audio processing functions.

Abstract: A wideband phase-lock loop frequency synthesizer (200) used in a radio transceiver capable of being reconfigured to operate in either a transmit, receive, or battery save mode. The wideband phase-lock loop frequency synthesizer (200) includes, a divide-by-two divider (205), quadrature detector (204), offset VCO (209) and offset mixer (207) for generating a quadrature phase modulated signal. Moreover, a programmable filter (211) is used for removing predetermined harmonic components of the offset mixed signal enabling the synthesizer to operate over a wide frequency range.

Abstract: A novel nucleotide sequence integration and targeting system is described. The system employs adeno-associated virus (AAV) derived vectors which include a selected nucleotide sequence bounded by AAV inverted terminal repeats (ITRs). An AAV rep coding region is also provided, enabling the targeted integration of the selected nucleotide sequence into the genome of a suitable target cell. The nucleotide sequence integration system of the present invention can deliver and integrate large segments of DNA into the genome of target cells. Further, the subject integration system provides the advantage of site-specific integration of the selected nucleotide sequences in a target cell genome, thereby avoiding insertional mutagenesis events experienced with prior systems.