Abstract: The present invention provides a detection article including at least one fluid control film layer having at least one microstructured major surface with a plurality of microchannels therein. The microchannels are configured for uninterrupted fluid flow of a fluid sample throughout the article. The film layer includes an acquisition zone for drawing the fluid sample into the plurality of microchannels at least by spontaneous fluid transport. The film layer also includes a detection zone having at least one detection element that facilitates detection of a characteristic of the fluid sample within at least one microchannel of the detection zone. The detection article may be formed from a plurality of film layers that are stacked to form a three-dimensional article.