Abstract: The present disclosure relates to a magnetizing portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetizing portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetizing portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.

Abstract: The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) having a magnetising portion (3) for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion (4) for retaining at least one sample vessel (5), wherein, the magnetising portion (3) is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (5); the sample vessel retaining portion (4) is configured to retain at least one sample vessel (5) such that at least a portion of the contents of the sample vessel (5) is visible to a user; and the sample vessel retaining portion (4) is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (3).

Abstract: The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) having a magnetising portion (3) for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion (4) for retaining at least one sample vessel (5), wherein, the magnetising portion (3) is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (5); the sample vessel retaining portion (4) is configured to retain at least one sample vessel (5) such that at least a portion of the contents of the sample vessel (5) is visible to a user; and the sample vessel retaining portion (4) is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (3).

Abstract: The present disclosure relates to a magnetising portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetising portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetising portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.

Abstract: Disclosed is a purified full-length cDNA molecule encoding putative serine kinase enzyme (PSKH-1), and the expression of the cDNA in a recombinant host cell to produce substantially purified PSKH-1, per se. Inactivation of PSKH-1 pre-MRNA or PSKH-1 mRNA halts DNA synthesis and cell division. Also disclosed are ribozymes capable of cleaving PSKH-1 pre-mRNA or mRNA and thus deactivating PSKH-1 translation. Ribozymes of the hammerhead and hairpin motifs, and various compositions containing same, are also disclosed. The ribozymes compositions are used in the treatment of mammalian patients suffering from diseases or medical conditions characterized by abnormal cell proliferation or growth such as cancer and various non-malignant diseases or medical conditions such as autoimmune diseases, allograft rejection and atherosclerosis.