Abstract: Methods are disclosed for preparing biologically active protein conjugates using negatively charged polymers that protect the protein within the conjugate so that it retains a substantial amount of its biological activity following conjugation (e.g., procedure in which a protein is conjugated to a pharmaceutical agent, a solid support, a reporter molecule, a group carrying a reporter molecule, an acylating agent, a chelating agent, a cross-linking agent, a targeting group, and a ligand and binding group). The invention also includes novel protein conjugates prepared by those methods.

Abstract: A method for producing a visible image of an object is disclosed in which a fermental plate is illuminated with ultrasonic waves and a latent image of the object is therefore produced therein which is developed using a solution of chromogenic substrates, comprising, for example, hydrogen peroxide and a water-soluble aromatic amine. An apparatus to practice the disclosed method comprises an ultrasonic generator to generate ultrasonic waves illuminating the object, a fermental plate to receive and convert the ultrasonic waves passed through the object to a visible image, and a tray filled with a developer to develop the latent image of the object in the fermental plate.