Patents by Inventor George R. Feehery
George R. Feehery has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230123635Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: ApplicationFiled: June 8, 2021Publication date: April 20, 2023Applicant: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu
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Publication number: 20230089237Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: ApplicationFiled: September 21, 2022Publication date: March 23, 2023Applicant: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu, Pierre O. Esteve
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Patent number: 11492667Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: GrantFiled: June 8, 2021Date of Patent: November 8, 2022Assignee: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu, Pierre O. Esteve
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Publication number: 20210388434Abstract: Compositions, methods and kits are provided for identifying the presence and location of a target in chromosomal DNA. A nicking endonuclease fused to a binding domain that binds to a constant region of an antibody (NEFP) is provided that may be used for binding to a target directly or via an antibody that binds to the target. The target may be a protein or structural feature of the DNA and its presence and location may correspond to a phenotype and/or pathology in a biopsy or other cell sample for diagnostic purposes. The background is reduced by the addition of a glycoaminoglycan (GAG) that reversibly inhibits binding of the NEFP to DNA. Nick translation in the presence of a strand displacing polymerase enables the incorporation of tagged nucleotides that (i) blocks re-nicking; (ii) facilitates immobilization of DNA fragments around the target for sequencing; and/or (iii) enables dye labelling of the chromosomal DNA within the cell nuclei for analysis by microscopy.Type: ApplicationFiled: June 8, 2021Publication date: December 16, 2021Applicant: New England Biolabs, Inc.Inventors: Sriharsa Pradhan, Hang Gyeong Chin, George R. Feehery, Shuang-Yong Xu, Vishnu Udayakumaran Nair Sunitha Kumary, Julie Beaulieu, Pierre O. Esteve
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Publication number: 20160032362Abstract: Compositions and methods are provided for facilitating the enrichment of single-stranded DNA containing methylated CpG in a mixture containing methylated and unmethylated DNA. The compositions relate to methylation-binding protein domains that selectively bind to methylated single strand DNA. In embodiments of the invention, the methylated DNA is eluted in 0.4M-0.6M NaCl while the unmethylated single strand DNA is eluted in less than 0.4M salt. The ability to readily enrich for methylated DNA permits high throughput sequencing of the methylated DNA and identification of abnormal methylation patterns associated with disease.Type: ApplicationFiled: February 10, 2015Publication date: February 4, 2016Inventors: George R. Feehery, Sriharsa Pradhan
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Patent number: 9145580Abstract: Compositions and methods are provided for enriching mitochondrial DNA and optionally chloroplast DNA from eukaryotic cells in a simple rapid method that provides greater than 100 fold enrichment. Affinity protein-coated substrate in a buffer is used to efficiently bind chromosomal DNA and thereby remove it from the buffer. Mitochondrial sequencing reads reveal that non-biased sequence selection providing representation of a substantial proportion of mitochondrial DNA in the eukaryotic cells analyzed.Type: GrantFiled: March 15, 2013Date of Patent: September 29, 2015Assignee: New England Biolabs, Inc.Inventors: George R. Feehery, Fiona Stewart, James McFarland, Sriharsa Pradhan
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Patent number: 8980553Abstract: Compositions and methods are provided for enriching non-target polynucleotides from a mixture of non-target and target polynucleotides where differences between the target polynucleotides and the non-target polynucleotides include the extent of modified bases that are present in a greater density in the target polynucleotides than in the non-target polynucleotides. This permits the target polynucleotides to be selectively and rapidly bound to an affinity matrix such as affinity protein-coated magnetic beads providing enrichment of the non-target polynucleotides in the supernatant. One use of this enrichment is to remove human genomic DNA from a mixture of DNAs obtained from human tissue samples to enrich for polynucleotides in a microbiome so as to characterize the microbiome by DNA sequencing.Type: GrantFiled: March 30, 2012Date of Patent: March 17, 2015Assignee: New England Biolabs, Inc.Inventors: George R. Feehery, Fiona Stewart, James McFarland, Sriharsa Pradhan
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Patent number: 8367331Abstract: Compositions and methods are provided for facilitating the enrichment of single-stranded DNA containing methylated CpG in a mixture containing methylated and unmethylated DNA. The compositions relate to methylation-binding protein domains that selectively bind to methylated single strand DNA. In embodiments of the invention, the methylated DNA is eluted in 0.4M-0.6M NaCl while the unmethylated single strand DNA is eluted in less than 0.4M salt. The ability to readily enrich for methylated DNA permits high throughput sequencing of the methylated DNA and identification of abnormal methylation patterns associated with disease.Type: GrantFiled: October 29, 2009Date of Patent: February 5, 2013Assignee: New England Biolabs, Inc.Inventors: George R. Feehery, Sriharsa Pradhan
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Publication number: 20120252009Abstract: Compositions and methods are provided for enriching non-target polynucleotides from a mixture of non-target and target polynucleotides where differences between the target polynucleotides and the non-target polynucleotides include the extent of modified bases that are present in a greater density in the target polynucleotides than in the non-target polynucleotides. This permits the target polynucleotides to be selectively and rapidly bound to an affinity matrix such as affinity protein-coated magnetic beads providing enrichment of the non-target polynucleotides in the supernatant. One use of this enrichment is to remove human genomic DNA from a mixture of DNAs obtained from human tissue samples to enrich for polynucleotides in a microbiome so as to characterize the microbiome by DNA sequencing.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: NEW ENGLAND BIOLABS, INC.Inventors: George R. Feehery, Fiona Stewart, James McFarland, Sriharsa Pradhan
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Publication number: 20100112585Abstract: Compositions and methods are provided for facilitating the enrichment of single-stranded DNA containing methylated CpG in a mixture containing methylated and unmethylated DNA. The compositions relate to methylation-binding protein domains that selectively bind to methylated single strand DNA. In embodiments of the invention, the methylated DNA is eluted in 0.4M-0.6M NaCl while the unmethylated single strand DNA is eluted in less than 0.4M salt. The ability to readily enrich for methylated DNA permits high throughput sequencing of the methylated DNA and identification of abnormal methylation patterns associated with disease.Type: ApplicationFiled: October 29, 2009Publication date: May 6, 2010Applicant: NEW ENGLAND BIOLABS, INC.Inventors: George R. Feehery, Sriharsa Pradhan
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Patent number: 4447924Abstract: A moisture control system is provided for controlling the amount of chemical added to a fabric. The system includes a suction pipe having a slot therein and a fabric positioned to pass over the slot. A vacuum source is connected through conduit means to the suction pipe. A source of liquid is provided including a predetermined percentage of chemical therein to be added to the fabric. Liquid from the liquid source is added to the fabric and the fabric containing the liquid from the liquid source is advanced over the suction pipe whereupon vacuum is applied to the fabric to deliquefy it. The vacuum source is set to provide a predetermined vacuum depending on the chemical concentration of the liquid and the composition of the fabric. As the fabric passes over the slot a change in the vacuum is sensed and the vacuum source is actuated to retain the vacuum to the predetermined condition thereby maintaining a constant moisture level and amount of added chemical in the fabric.Type: GrantFiled: February 18, 1982Date of Patent: May 15, 1984Assignee: Albany International Corp.Inventors: Joseph A. Bolton, George R. Feehery