Abstract: A method of treating cancer by administering to a subject in need thereof a therapeutically effective amount of a tyrosine kinase inhibitor and an NF-?B inhibitor is described. The method is useful for treating subjects having cancer that has developed resistance to tyrosine kinase inhibitors.

Abstract: Methods of identifying a gene whose product modulates a control phenotype of interest are provided. The methods comprise introducing a promoter insertion construct of the present invention into the genomes of a collection of host cells having the control phenotype of interest; selecting mutagenized cells exhibiting a mutant phenotype to provide a pool of mutant cells; treating the mutant cells with the disrupting agent having recombinase activity; detecting changes or the lack thereof in the linkage between the promoter element and downstream host genomic DNA sequences in treated mutant cells, or in both treated and untreated mutant cells; and correlating the changes or lack thereof in the linkage between the promoter element and the downstream host genomic DNA sequences in treated mutant cells, or in both untreated and treated mutant cells, with the phenotypes of said cells. Also provided are compositions that are used in the present methods.

Abstract: Mutant cell lines which have lost their ability to respond to IL-1 are provided. In one embodiment, the mutant cell line lacks or is essentially free of IL-1 receptor associate kinase (IRAK), an IL-1 signaling pathway component. The present invention also provides a method for making mutant cell lines that are unresponsive to IL-1, TNF, or to both cytokines.

Abstract: Improvements in the transfer and detection of separated nucleic acids, both RNA and DNA, are provided. For analysis of large DNA, the molecular weight segregated fractions of DNA are depurinated and fragmented to provide fractions having less than about 2 kb as single strands. With both RNA and DNA, the nucleic acid fractions are transferred after resolution to a chemically treated substrate and covalently affixed to the substrate. The resulting nucleotides affixed to the substrate are hybridized with labeled nucleotide probes and a volume exclusion agent, particularly a water soluble ionic polymer.