Patents by Inventor Gerald E. Jaffe
Gerald E. Jaffe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5905031Abstract: The necessity to detect blast cells in a patient sample to determine a treatment regime for leukemia has long been desired. The present invention relates to a method and assay reagents for identification of blast cells in a fluid sample. The method further enables the determination of the lineage of the blast cells. The new method provides a determination of blast cells in less time than prior art methods.Type: GrantFiled: November 1, 1996Date of Patent: May 18, 1999Assignee: Coulter International Corp.Inventors: Nazle Kuylen, Harold R. Crews, Gerald E. Jaffe, Maria Barcelon, Francis J. Lucas, Nancy M. Garcia
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Patent number: 5871946Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.Type: GrantFiled: May 18, 1995Date of Patent: February 16, 1999Assignee: Coulter CorporationInventors: Frank J. Lucas, Gerald E. Jaffe, Steven Bott, James H. Carter
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Patent number: 5849513Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.Type: GrantFiled: July 31, 1997Date of Patent: December 15, 1998Assignee: Coulter CorporationInventors: Gerald E. Jaffe, Frank J. Lucas, James H. Carter
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Patent number: 5776720Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.Type: GrantFiled: May 18, 1995Date of Patent: July 7, 1998Assignee: Coulter CorporationInventors: Gerald E. Jaffe, Frank J. Lucas, James H. Carter
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Patent number: 5733719Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.Type: GrantFiled: May 18, 1995Date of Patent: March 31, 1998Assignee: Coulter CorporationInventors: Gerald E. Jaffe, Frank J. Lucas, James H. Carter
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Patent number: 5698411Abstract: A method for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound is also disclosed.Type: GrantFiled: May 18, 1995Date of Patent: December 16, 1997Assignee: Coulter CorporationInventors: Frank J. Lucas, Gerald E. Jaffe, Steven E. Bott, James H. Carter
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Patent number: 4801534Abstract: Water soluble xanthylium derivative substrates of rhodamine 110 and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen to monitor the pathways of blood coagulation.Type: GrantFiled: June 18, 1987Date of Patent: January 31, 1989Assignee: Coulter Electronics, Inc.Inventors: Gary A. Mitchell, Gerald E. Jaffe, Marilyn M. Solorzano
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Patent number: 4694070Abstract: Water soluble xanthylium derivative substrates of rhodamine 110 and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen or monitor the pathways of blood coagulation.Type: GrantFiled: May 28, 1985Date of Patent: September 15, 1987Assignee: Coulter Electronics, Inc.Inventors: Gary A. Mitchell, Gerald E. Jaffe, Marilyn M. Solorzano
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Patent number: 4087331Abstract: A colorimetric method for the determination of gamma-glutamyl transpeptidase enzyme activity in a biological fluid comprises catalytically reacting the enzyme in the fluid with a substrate of a water-soluble salt of a compound selected from the group consisting of L-gamma-glutamyl-3-sulfoanilide and L-gamma-glutamyl-4-sulfoanilide in aqueous solution to produce the corresponding aniline sulfonic acid salt as a cleavage product, diazotizing the cleavage product, and coupling the diazotized product with a coupling compound to produce a dye having a color intensity proportional to the enzyme activity.Type: GrantFiled: January 12, 1977Date of Patent: May 2, 1978Assignee: Coulter Electronics, Inc.Inventors: Giovanni Bucolo, Mathew M. Madappally, Gerald E. Jaffe