Abstract: Disclosed are methods for introducing nucleic acid constructs called “landing pads” in plant genes for the insertion of transgenes, and methods for introducing the transgenes into the landing pads. Transgenic plants and plant parts produced by the methods, and seeds derived from the plants, are also disclosed.

Abstract: The present invention relates to modified tetracycline-inducible cassettes for controlling gene expression in organisms, particularly plants. Specifically, the invention provides novel tetracycline repressor and operator cassettes. The invention preferably provides a tetracycline-inducible expression cassette comprising both the tetracycline repressor and operator cassettes of the present invention wherein the repressor and operator cassettes are located on a single plasmid and/or vector. Also provided is a method of producing herbicide resistant plants using the modified tetracycline inducible cassettes of the present invention to control the expression of a herbicide resistance gene. Moreover, a method for identifying novel tetracycline analogs and/or functional equivalents using the modified tetracycline inducible cassettes of the present invention is also presented.

Abstract: A nuclear scaffold attachment region isolated from a tobacco gene, and a method of making recombinant cells having increased levels of expression of foreign genes therein, are described. The method comprises transforming the cell with a DNA construct comprising in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region of the nucleotide sequence provided herein, positioned either 5' to the transcription initiation region or 3' to the structural gene. DNA constructs and vectors employed in carrying out the foregoing method are also discussed.