Abstract: A method for manufacturing a catheter (102) including a rapid exchange port (157). The method can include the steps of creating a port (258) in a catheter shaft (210), inserting a port tube (360) into the port (358), skiving the port tube (360) so that it is flush with the catheter shaft (310), inserting a guidewire lumen (718) into the port tube (760), coupling the guidewire lumen (718) to the port tube (760), and skiving the guidewire lumen (718) so that it is flush with the catheter shaft. The method can also include the steps of inserting a port mandrel (462) into the port tube (460), inserting a catheter mandrel (464) into the catheter shaft (410), positioning a heat shrink (465) over a portion of the catheter shaft (410), applying heat to the heat shrink (465), removing the port mandrel (462) from the port tube (460), removing the catheter mandrel from (464) the catheter shaft (410), and sealing a gap between the guidewire lumen (718) and the port tube (760).

Abstract: The invention provides evolved red-shifted smURFPs. In some embodiments, the smURFPs are characterized by a) increased Fluorescence of smURFP as compared to infrared FPs IFP1.4 and iRFP713; b) expressing efficiently with minimal toxicity; c) does not require a lyase to covalently attach its chromophore, wherein the chromophore is biliverdin; d) exhibit a wavelengths longer than attainable with jellyfish- or coral-derived FPs using smURFP and IFP2.0; e) allows for functional fusion to hCdt1(30/120) as compared to jellyfish- or coral-derived FPs mAG, eGFP, and mRFP1 which are nonfunctional; and f) allows for deep tissue imaging.

Abstract: The invention provides evolved red-shifted smURFPs. In some embodiments, the smURFPs are characterized by a) increased Fluorescence of smURFP as compared to infrared FPs IFP1.4 and iRFP713; b) expressing efficiently with minimal toxicity; c) does not require a lyase to covalently attach its chromophore, wherein the chromophore is biliverdin; d) exhibit a wavelengths longer than attainable with jellyfish- or coral-derived FPs using smURFP and IFP2.0; e) allows for functional fusion to hCdt1(30/120) as compared to jellyfish- or coral-derived FPs mAG, eGFP, and mRFP1 which are nonfunctional; and f) allows for deep tissue imaging.