Abstract: The invention relates generally to peptide biomarkers with specific ionization characteristics to directly quantify one or more transgenic target proteins in biological samples, including transgenic plant samples, by liquid chromatography coupled tandem mass spectrometry multiple reaction monitoring (MRM). The peptide biomarkers in combination with MRM-based methods may be used to quantify a single transgenic target protein or multiple transgenic target proteins within a stacked transgenic crop, such as maize, utilizing selected peptide biomarkers either alone or in combination. The present disclosure allows for broad based, reliable quantitation in different biological matrices, including plant matrices. The peptide biomarkers of the invention can further be used as trait biomarkers to support identification and/or selection of specific transgenic Events. Also provided are different peptide biomarker combinations that can be used to perform the methods of the invention.

Abstract: The present invention includes non-transgenic domesticated tomato plants that express flavonols in the flesh and peel of the tomato fruit, and includes the seeds and fruits of such plants. The method of the invention includes assaying tomato plants for expression of one or more of the flavonol biosynthesis genes in the flesh and/or expression of CHI in the peel. The method of the invention includes selecting wild tomato species that express CHI in the fruit peel, and/or one or more genes of the flavonol biosynthesis pathway in the flesh, and introgressing the genetic factors responsible for this expression from the wild tomato species into a domesticated tomato plant using traditional breeding techniques.

Abstract: Improved compositions and methods for controlling coleopteran pests are disclosed. In particular, novel combinations of insecticidal proteins having improved toxicity to coleopteran insect pests such as corn rootworm are provided. Further, a method of killing or controlling coleopteran insect pests using the compositions of the invention is disclosed.

Abstract: The present invention includes non-transgenic domesticated tomato plants that express flavonols in the flesh and peel of the tomato fruit, and includes the seeds and fruits of such plants. The method of the invention includes assaying tomato plants for expression of one or more of the flavonol biosynthesis genes in the flesh and/or expression of CHI in the peel. The method of the invention includes selecting wild tomato species that express CHI in the fruit peel, and/or one or more genes of the flavonol biosynthesis pathway in the flesh, and introgressing the genetic factors responsible for this expression from the wild tomato species into a domesticated tomato plant using traditional breeding techniques.

Abstract: The present invention includes non-transgenic domesticated tomato plants that express flavonols in the flesh and peel of the tomato fruit, and includes the seeds and fruits of such plants. The method of the invention includes assaying tomato plants for expression of one or more of the flavonol biosynthesis genes in the flesh and/or expression of CHI in the peel. The method of the invention includes selecting wild tomato species that express CHI in the fruit peel, and/or one or more genes of the flavonol biosynthesis pathway in the flesh, and introgressing the genetic factors responsible for this expression from the wild tomato species into a domesticated tomato plant using traditional breeding techniques.

Abstract: The present invention includes non-transgenic domesticated tomato plants that express flavonols in the flesh and peel of the tomato unit, and includes the seeds and fruits of such plants. The method of the invention includes assaying tomato plants for expression of one or more of the flavonol biosynthesis genes in the flesh and/or expression of CHI in the peel. The method of the invention includes selecting wild tomato species that express CHI in the fruit peel, and/or one or more genes of the flavonol biosynthesis pathway in the flesh, and introgressing the genetic factors responsible for this expression from the wild tomato species into a domesticated tomato plant using traditional breeding techniques.