Abstract: An in vitro method to produce a glycoside is described which includes the steps of contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate or alpha-galactose-1-phosphate and an acceptor, and glycosylating the acceptor. The acceptor may be an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid. Alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids are produced when alpha-glucose-1-phosphate is used as donor. Corresponding lactosides are produced when alpha-galactose-1-phosphate is used as donor.

Abstract: The present invention relates to the biocatalytic production of glycosides. In particular, the invention discloses a method to produce alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids via the usage of a cellodextrin phosphorylase (CDP) derived from Clostridium stercorarium (CsCDP). The method of the present invention is feasible for use in industrial glycosylation processes and has advantageous properties compared to classical as chemical glycosylation reactions. Moreover, said method can also be employed to produce corresponding lactosides such a lactolipids when a-galactose-1-phosphate instead of a-glucose-1-phosphate is used as a donor.