Abstract: The present invention is directed to optical bio-discs, methods, and optical disc drives for clinical diagnostics. The present invention is further directed to methods for determining the quantity of a specific type of blood cell in a biological sample including on-disc sample preparation and processing, providing a pre-processed sample onto a capture zone, removing portions of the sample that are not bound in the capture zone, and counting bound cells. Also disclosed are fluidic circuits for processing blood samples for use in a cluster designation marker assay in the optical bio-disc.

Abstract: The present invention is directed to optical bio-discs, methods, and optical disc drives for clinical diagnostics. The present invention is further directed to methods for determining the quantity of a specific type of blood cell in a biological sample including on-disc sample preparation and processing, providing a pre-processed sample onto a capture zone, removing portions of the sample that are not bound in the capture zone, and counting bound cells. Also disclosed are fluidic circuits for processing blood samples for use in a cluster designation marker assay in the optical bio-disc.

Abstract: A clinical diagnostic assay is performed on an optical bio disc and is read with a disc drive. More specifically this invention relates to capture layer assemblies for cellular assays using optical bio-discs. The current invention includes methods for determining the quality and quantity of a specific type of cell in a biological sample. These methods includes binding capture agents to a capture zone on the disc, providing a sample to the capture zone, remove portions of the sample that are not bound in the capture zone, and counting bound cells. The current invention also includes methods and apparatus for performing a cluster designation marker assay using an optical disc and disc drive and method for making optical analysis discs for performing such cluster designation assays.

Abstract: A clinical diagnostic assay is performed on an optical bio disc and is read with a disc drive. Methods and apparatus for detecting and quantifying specific blood cell analytes in biological samples using optical bio disc is disclosed. The method for determining the quantity of a specific type of blood cell in a biological sample includes binding an antibody to a capture zone on the disc, providing a sample to the capture zone, remove portions of the sample that are not bound in the capture zone, and counting bound cells. Also described is method and apparatus for performing a cluster designation count with an optical disc and disc drive and method for making an optical assay disc for performing such cluster designation count.

Abstract: The present invention relates in general to biological assays and diagnostic assays and, in particular, to methods and apparatuses for imaging cells using an optical bio-disc system. More specifically, but without restriction to the particular embodiments hereinafter described in accordance with the best mode of practice, this invention relates to methods for identifying and quantitating cells including white blood cells based on the morphology of their nucleus using stains that absorb electromagnetic radiation at pre-determined wavelengths in conjunction with optical bio discs.

Abstract: A clinical diagnostic assay is performed on an optical bio disc and is read with a disc drive. A method for determining the quantity of a specific type of blood cell in a biological sample includes binding an antibody to a capture zone on the disc, providing a sample to the capture zone, remove portions of the sample that are not bound in the capture zone, and counting bound cells.