Abstract: Described are methods for inactivating adenine nucleotide transporter proteins in specific tissues of a transgenic nonhuman animal using a conditional knockin/knockout technology such as the Cre-LoxP, Flip-FLP recombinase, or Tet-on/off technologies. Specifically, the Ant2 gene is functionally inactivated in a mouse in liver, with or without the concurrent inactivation of the Ant1 gene. The result is an animal in which the Ant2 gene and accompanying ANT 2 protein is absent in one or more tissues, either in the presence or absence of the Ant1 gene and accompanying protein. The resulting animals, cells, mitochondria, and subcelluar fractions such as the mitochondrial permeability transition pore can then be used to identify agents that affect animal and/or subcellular function via a direct or indirect interaction with the ANT2 protein and/or its Ant2 gene.

Abstract: Described are methods for inactivating adenine nucleotide transporter proteins in specific tissues of a transgenic nonhuman animal using a conditional knockin/knockout technology such as the Cre-LoxP, Flip-FLP recombinase, or Tet-on/off technologies. Specifically, the Ant2 gene is functionally inactivated in a mouse in liver, with or without the concurrent inactivation of the Ant1 gene. The result is an animal in which the Ant2 gene and accompanying ANT2 protein is absent in one or more tissues, either in the presence or absence of the Ant1 gene and accompanying ANT1 protein. The resulting animals, cells, mitochondria, and subcelluar fractions such as the mitochondrial permeability transition pore can then be used to identify agents that affect animal and/or subcellular function via a direct or indirect interaction with the ANT2 protein and/or its Ant2 gene.

Abstract: A fluid supply and reservoir is proved which may be used in conjunction with a clothes refreshing appliance. A fluid cartridge has a top wall to which the nozzle assembly may be secured, and a supply of pressurized air is connected to the nozzle assembly to draw fluid from the reservoir into the nozzle assembly through a siphon tube by a venturi action. The air supply may be provided through an air supply arrangement which includes guide walls for positioning the nozzle assembly relative to the air supply, and a resiliently mounted air supply tube for mating with an air supply opening in the nozzle assembly. An arrangement may also be provided in the reservoir to assure a constant fluid pressure therein to provide a constant flow of fluid through the nozzle assembly.

Abstract: A fluid supply and reservoir is proved which may be used in conjunction with a clothes refreshing appliance. A fluid cartridge has a top wall to which the nozzle assembly may be secured, and a supply of pressurized air is connected to the nozzle assembly to draw fluid from the reservoir into the nozzle assembly through a siphon tube by a venturi action. The air supply may be provided through an air supply arrangement which includes guide walls for positioning the nozzle assembly relative to the air supply, and a resiliently mounted air supply tube for mating with an air supply opening in the nozzle assembly.

Abstract: A beverage holder composed of hollow cylindrical upper and lower parts, where the parts are rotatable with respect to one another. The upper and lower parts are connected with one another by a plurality of rod-shaped connecting elements (rods). When the two parts are rotated relative to one another, the rods are tilted, such that the inside diameter inside the hollow cylindrical upper and lower parts is reduced. In this manner, a container inserted into the beverage holder is reliably clamped. The rod-shaped connecting elements may be designed "in the manner of a stocking." When an impermeable material is used and the rod-shaped connecting elements are attached in a sealing fashion to the hollow cylindrical assembly and/or the ring, the cylindrical assembly then becomes suitable for allowing a liquid or a gaseous media to flow through it.