Abstract: In some instances, the application provides methods of culturing host cells comprising a gene encoding a recombinant N protein in a cell culture medium, wherein the cell culture medium comprises: (i) iron at a concentration of less than 1200 ?M; and (ii) citrate at a concentration of less than 2400 ?M. In some instances, the methods comprise culturing host cells expressing a recombinant protein in a cell culture medium at a first temperature; and decreasing the first temperature to a second temperature. In some instances, the methods comprise purifying the recombinant protein by a carbon depth filtration.

Abstract: A securing apparatus may be configured to secure a wearable item to any one of a variety of different graspable articles, in a suitable manner. The securing apparatus may include a securing portion, having multiple securing members. The stiffness of one of the securing members may differ from the stiffness of another securing member. The securing members may be cantilevers. In some embodiments, the anchored end of one of the cantilevers may be attached to the deflecting end of another cantilever. One of the cantilevers may deform plastically due to repeated usage with relatively thick garments, though, by employing at least one additional cantilever in an appropriate manner, the ability to grip thinner garments in a suitable manner remains uncompromised.

Abstract: A process for the preparation of a static dissipative material from a substrate material formed of a thermoformable polymeric matrix of low electrical conductivity. The substrate material is contacted with a solution of an aniline-based monomeric or oligomeric conductant which is selected from the group consisting of aniline and substituted aniline components. The aniline-based conductant is dissolved in a solvent and maintained in contact with the substrate material for a period of time to permit at least partial infusion of the conductant into the polymeric matrix. Subsequent to the contact step, the substrate material is subjected to a curing step in which the aniline-based segments introduced into the polymeric matrix are at least partially polymerized. The polymerization of the infused aniline segments can be carried out in an oxidizing environment or by subjecting the infused substrate material with electromagnetic radiation in the ultraviolet range.

Abstract: A U.V. curable composition for application to a substrate surface to provide a static dissipative coating which comprises a conductive matrix constituent having a crown type ligand selected from the group consisting of podands, coronands and cryptands in a guest/host relationship with a conductive dopant moiety. Such ligands included those provided by functionalized podands and functionalized coronands. The formulation further includes a polymer matrix constituent formed of a base oligomer system and a crosslinking agent which is reactive with the base oligomer system to form a crosslinked resin matrix which incorporates the conductive matrix constituent. A low viscosity diluent functions as a solvating liquid for the crosslinking agent and the base oligomer system. The formulation further includes a photoinitiator of the free radical or cationic type to initiate polymerization of the composition upon the application of electromagnetic radiation in the ultraviolet range.

Abstract: A composition and method for the removal of ionic salt deposits from a surface using an organic solvent of low polarity and a complexing agent. The complexing agent is chosen from a group which includes podands, cryptands, and coronands. The method provides nearly complete removal of ionic salt deposits with a single washing of the surface with the low polar cleaning mixture.